29H,31H-phthalocyanine

Sediment toxicity

Link to relevant study record(s)

Reference

Endpoint:

sediment toxicity: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 225 (Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: Pigment Green 7, Batch Number: V.9358
- Purity, including information on contaminants, isomers, etc.: 100%


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient conditions

FORM AS APPLIED IN THE TEST (if different from that of starting material)
- Specify the relevant form characteristics if different from those in the starting material, such as state of aggregation, shape of particles or particle size distribution: green powder

Analytical monitoring:

no

Vehicle:

no

Details on sediment and application:

PREPARATION OF SPIKED SEDIMENT
- Method of mixing: the test substance was mixed with 10 g quartz sand. The sand was mixed with artificial sediment.

Test organisms (species):

Lumbriculus variegatus

Details on test organisms:

TEST ORGANISM
- Source: Bundesanstalt fuer Gewaesserkunde (BfG), Koblenz, Germany
- Breeding conditions: performed on quartz sand in dilution w ater at the test facility at 20 ± 2 °C and diffuse light (100 - 500 lx, 16 h photoperiod daily).
- Feeding during test
- Food type: Powder of Urtica dioica
- Amount: 0.4 % of sediment dw
- Frequency: mixed with the sediment at application day

ACCLIMATION
- Type and amount of food: TetraMin flake food (Tetra)
- Feeding frequency: 2 times per week

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Type of sediment:

artificial sediment

Limit test:

yes

Duration:

28 d

Exposure phase:

total exposure duration

Hardness:

342 - 406 mg/l CaCO3

Test temperature:

20.3 - 21.1 °C

pH:

7.56 - 8.41

Dissolved oxygen:

7.08 - 8.41 mg/l

Nominal and measured concentrations:

Nominal test concentration: 1000 mg test item/kg sedimend try w eight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): glass vessel, 6 cm diameter (250 ml)
- Sediment volume: 3 cm
- Weight of wet sediment with and without pore water: 45 g
- Overlying water volume: 150 ml
- Aeration: yes
- Replacement of evaporated test water, if any: weekly

EXPOSURE REGIME
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 6
- No. of replicates per control / vehicle control: 6


OVERLYING WATER CHARACTERISTCS
- Type of water (e.g. deionized, ground water, sea water, Elendt medium acc. to OECD 219): reconstituted demineralised water (CaCl2 x 2 H2O, MsSO4x 7 H2O, NaHCO3, KCl


CHARACTERIZATION OF (ARTIFICIAL; delete if not applicable) SEDIMENT
- % dry weight of sphagnum moss peat: 5 %
- Composition (if artificial substrate): - 20 % kaolin, 75 %, quartz sand, 0.47 % calcium carbonate

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 100-500 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
reproduction, behaviour, biomass, mortality


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/kg sediment dw
- Results used to determine the conditions for the definitive study: LC50 > 1000 mg/kg sediment dw

Reference substance (positive control):

yes

Remarks:

Potassium chloride

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, behaviour, biomass, reproduction

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

EC50= 4.06 mg/l

Validity criteria:

- At the end of the test,t he oxygenc oncentratioinn the overlying dilutionw aters houldb e > 70 % of the air saturationv alue correspondintgo 6.2 mg/L.

- The pH-values houldb e between6 and 9 in the overlyingte st water at start and end of the test.

- ln the control group, the average number of living worms per replicates houldi ncreaseb y a factoro f at least1 .8.

Validity criteria fulfilled:

yes

Description of key information

The substance is not toxic to sediment-dwelling organisms, with a NOEC ≥ 1000 mg/kg sediment dry weight.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater sediment:

1 000 mg/kg sediment dw

Additional information

All substances in this category share a similar structure with a molecular weight > 500 g/mol. In addition, the substances have a low potential to adsorb to soil and suspended matter. Based on the high molecular weight and insolubility, the substances in this category will not be able to penetrate biological membranes. This is confirmed by the environmental studies on sediment-dwelling organisms for one substance in this category: CAS 1328-53-6. The environmental toxicity data can be used for read-across to all substances in this category.

The effect of substance CAS 1328-53-6 on the sediment-dwelling organism Lumbriculus variegatus was tested in a sediment-water system for 28 days according to OECD TG 225. The 28-d NOEC was determined to be ≥ 1000 mg/kg sediment dry weight for mortality behaviour, biomass, and reproduction in this limit test (BASF 2010, K1).

The NOEC was equal to or above the tested concentration. Therefore, it is concluded that all substances in the category are not toxic to sediment-dwelling organisms, with a NOEC ≥ 1000 mg/kg sediment dry weight.