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EC number: 217-895-0 | CAS number: 2001-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to German national standards (DIN)
- Justification for type of information:
- Read across is based on a structurally similar material. An analogue justification is attached in section 13 of dataset.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 11
- Principles of method if other than guideline:
- STATISTICS
Spearman-Kaerber (1984) - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by dissolving 2000 mg test substance in dilution water. The test concentrations were then prepared by dilution with test water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Strain: STRAUS
Breeder: Dr. U. Noack-Laboratorium
Hildesheim Source: Bundesgesundheitsamt, Berlin
Method of breeding: single breeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 21 °C
- Dissolved oxygen:
- 8.0 - 8.2 mg/L
- Nominal and measured concentrations:
- Test concentrations (nominal): 58, 100, 180, 320 and 580 mg/L
- Details on test conditions:
- TEST SYSTEM
Type: open beaker (50 mL)
Aeration: none
No. of organisms per vessel: 5
No. of replicates: 4
Controls: negative control
Highest concentration after pH adjustment
TEST MEDIUM / WATER PARAMETERS
Dilution water: according to DIN 38412 L11, 8.2
Conductivity: 658 µS/cm
Ca/Mg ratio: 5:1
Na/K ratio: 20:1
TEST CONDITIONS
Adjustment of pH: none
Illumination: 10 uE/qm*s pH
During test: 3.85 - 7.90
The number of immobilized animals were recorded after 3, 6, 24, and 48 hours after test start.
The parameters pH, temperature and oxygen concentration were determined at the end of the test. - Reference substance (positive control):
- yes
- Remarks:
- Potassium chromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 100-180 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No pH effect observed.
- Results with reference substance (positive control):
- Reference substance: Potassium chromate
EC50 (24 h): 0.9 mg/L
Date: 12 Sep - 13 Sep 1989 - Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to national standards (DIN)
- Justification for type of information:
- Read across is based on a structurally similar material. An analogue justification is attached in section 13 of dataset.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: DIN 38412, part 11
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna
Origin: wild population
Breeding: The clone with the highest number of offspring was used to establish a continuous daphnia breed under standardized laboratory conditions. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 310 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 1 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to international standard.
- Justification for type of information:
- Read across is based on a structurally similar material. An analogue justification is attached in section 13 of dataset.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 610 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Referenceopen allclose all
pH values at the end of the test
--------------------------------
580 mg/L 3.85
320 mg/L 4.02
180 mg/L 4.40
100 mg/L 7.30
58 mg/L 7.73
control 7.90
control-pH 7.49
--------------------------------
Nominal Mean number of mobile daphnids after
conc. 24h 48h
[mg/l]
-----------------------------------------------------------
580 0/20 0/20
320 0/20 0/20
180 0/20 0/20
100 20/20 19/20
58 20/20 19/20
control 20/20 19/20
pH-control 0/20 0/20
-----------------------------------------------------------
In this short-term test, Ca was present in overstoichiometric amounts. Thus, in the test system the Ca-complex was formed.
EC50(24h) calculated (confidence interval 95 %):
----------------------------------------------------
EDTA 610 mg/l (570-640)
Mn(II)+EDTA 940 mg/l (800-1000)
Fe(III)+EDTA 17 mg/l (13-21)
Cu(II)+EDTA 38 mg/l (31-45)
Zn(II)+EDTA 910 mg/l (840-980)
Hg(II)+EDTA 0.00032 mg/l (0.00022-0.00052)
Cd(II)+EDTA 310 mg/l (290-330)
----------------------------------------------------
All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal. To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were converted to mg/L EDTA.
EC 50 values with 95% confidence limits were calculated by a probit analysis computer program, version 2.3 (National Swedish Environmental Protection Agency).
The EC50 values of Fe3+, Cu2+, Mn2+, Zn2+, Cd2+ and Hg2+ complexed by EDTA were compared with those of the respective uncomplexed metals and free EDTA.
The experiments gave the following toxicity order: Hg2+, Cu2+, Cd2+, Zn2+, Fe3+, Mn2+ (higher to lower toxicity).
After complexation, the toxicities were 1.1-1700 (EDTA) times lower. With Hg2+ complexation did not decrease the toxicity. Compared with the toxicity of free EDTA, metal complexation increased the toxicity of EDTA.
Description of key information
With high probability the test substance is acutely not harmful to aquatic invertebrates.
Key value for chemical safety assessment
Additional information
Tests on acute toxicity with Daphnia magna resulted in EC50 values well above 100 mg/L [EU Risk Assessment, 2004]. Exemplarily in an non-GLP study according to DIN 38412 the measured EC50-value for Na2EDTA was 140 mg/L [BASF AG, 1989], for justification for read-across see IUCLID 5, Chapter 13.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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