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EC number: 211-536-1 | CAS number: 659-70-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-12-09 to 2009-05-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): wastewater treatment plant of Geneva (Peney-Dessous), sampled on day of test start
- Pretreatment: washed with mineral medium and centrifugated three times, kept aerobic
- Concentration of sludge: 30 mg dw/L in test medium - Duration of test (contact time):
- 29 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to OECD 301F
- Test temperature: 22°C
- pH: 7.6
- pH adjusted: yes
- Suspended solids concentration: 1.53 g dw/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Oxitop Control System
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- analytical purity >99.0%
- Preliminary study:
- yes
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70
- Sampling time:
- 28 d
- Details on results:
- The test substance showed no inhibitory effects on the microorganisms at the test concentration of 100 mg/L as estimated in the toxicity control.
- Results with reference substance:
- Degradability of sodium benzoate obtained from O2 consumption was 86% after 28 d. Therefore, the study is valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: biodegradable
- Conclusions:
- The biodegradation of the test item was determined in a GLP study according to OECD 301 F. Within the test period of 28 days, a degradation of 70 % was determined for the test substance. Furthermore, at the test concentration of 100 mg/L, no inhibitory effects on microorganisms from the test substance were observed as determined in the toxicity control. Given the 10 day window criteria is not fullfilled (11% biodegradation on day 2 and 47 % degradation on day 12), the substance is not readily biodegradable according to OECD criteria.
Reference
Biodegradation, mean [%] after n days |
||||||
|
1 |
2 |
12 |
21 |
28 |
29 |
Test substance* (mean of 2 values) |
6 |
11 |
47 |
67 |
70 |
70 |
Reference substance (mean of 2 values)* |
67 |
75 |
80 |
83 |
86 |
88 |
*Blank value considered
BOD, mean in [mg/O2/L] after n days |
||||||
|
1 |
2 |
12 |
21 |
28 |
29 |
Test substance* (mean of 2 values) |
15.8 |
27.5 |
123.0 |
173.4 |
180.9 |
181.4 |
Reference substance* (mean of 2 values) |
111.1 |
124.2 |
133.8 |
138.3 |
143.4 |
146.4 |
Toxicity control (mean of 2 values) |
126.1 |
167.4 |
235.9 |
283.3 |
324.6 |
327.1 |
*Blank value considered
Description of key information
The biodegradation of test item was determined in two GLP studies according to OECD 301 F and OECD 301 D.
Key study: Within the test period of 28 days, a degradation of 70 % was determined for the test substance. In addition, at the test concentration of 100 mg/L, no inhibitory effects on the microorganisms from the test substance were observed as estimated in the toxicity control. Given the 10 day window criteria is not fullfilled (11% biodegradation on day 2 and 47 % degradation on day 12), the substance is not readily biodegradable according to OECD criteria.
Supporting study: Within the test period of 28 days a degradation of 24% was determined for the test substance. No inhibitory effects on microorganisms at the tested concentration of 2.9. mg/L reference substance and 2.7 mg/L test substance were observed due to a degradation of 49 % in 28 days measured as O2 consumption. According to OECD criteria the substance is not readily biodegradable.
Based on the study results and according to the criteria given in the ECHA Guidance (Guidance on the Application of the CLP Criteria) from 2015, the test substance can be considered for classification as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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