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EC number: 401-950-2 | CAS number: 31506-43-1 3-(DIMETHYLAMINO)PROPYL UREA; HST 2844
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 March - 19 September 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 2012
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: Manufacturer, UA21412002
- Purity, including information on contaminants, isomers, etc.: Content: 75% 3-(N,N-Dimethyl)propyl-urea and 25% Polyethylenglycol
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store container tightly closed at a well-ventilated place. Store in the original container. Do not store with acids. - Analytical monitoring:
- yes
- Details on sampling:
- The test item concentrations of PU-2021-971 were analytically verified via LC-MS/MS three times during the test (once within a period of 7 days) in the fresh media at the start of an exposure-renewal interval (0 hours; on test days 0, 7 and 14) as well as in the old media at the end of an exposure-renewal interval (48 hours; on test days 2, 9 and 16) in all test item concentrations and in the control.
Details of the analytical method are presented in the attachement in the results section - overall remarks - Vehicle:
- yes
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Justification for species other than prescribed by test guideline: Daphnia magna is the preferred species in accordance with the test guideline
- Age at study initiation (mean and range, SD): Less than 24 hours old daphnids from a healthy stock were used for the study. To achieve this, juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within the following period were used for the test. No first brood progeny was used for the test.
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD):
- Feeding during test
- Food type: Daily feeding with unicellular green algae, e.g., Pseudokirchneriella subcapitata and Desmodesmus subspicatus
daphnia and day
- Amount: max. 0.2 mg C /day
- Frequency: at least 5 times per week ad libitum
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- The total hardness should be above 140 mg/L CaCo3
- Test temperature:
- The temperature was in the range of 18 – 22 °C and did not vary by more than 2°C.
- pH:
- 6-9
- Dissolved oxygen:
- The oxigen concentration should be > 3 mg/L at the start and during the test period.
- Nominal and measured concentrations:
- Nominal concentrations: 0.954 - 3.05 - 9.77 - 31.3 - 100 mg/L. Measured concentrations: The measured concentrations of the test item in the fresh media were between 99 and 117% of the
nominal values. In the aged media after 48 hours were the concentrations were in the range of 107 to 119% of the nominal values. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 (ID) x 8 (H) cm), 100 mL capacity, loosely covered with watch glasses
- Type (delete if not applicable): closed
- Aeration: Test vessels were not aerated during the test.
- Renewal rate of test solution: The test solutions were renewed 3 times per week (after 48 or test solutions 72 hours). For this purpose, a second set of test vessels were filled with the freshly prepared test solutions and the daphnids were transferred by pipette
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): one daphnid per replicate
OTHER TEST CONDITIONS
- Adjustment of pH: The pH value should be in the range of 6-9. Deviation between the initial and the final pH-values should be < 1.5 units.
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Max. 1500 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality of parental daphnids: The number of parental daphnids, which were immobilized or dying during the test and the dates of death were observed and recorded once a day at least at the same time as the offspring was counted. Dead daphnids were removed as soon as they were detected.
Neonates: First appearance of juveniles was checked daily. The number of neonates (alive and dead progeny) was counted, and dates of release were recorded once a day from the first day of appearance of neonates until the end of the exposure. The neonates (alive and dead) were removed after counting and before addition of algae to prevent them from consuming food intended for the adults. The number of aborted eggs or dead offspring and dates of observation were recorded.
Additional observations/Condition of parental daphnids: Abnormalities (e.g., abnormal swimming behavior, number of males and winter eggs) were observed and recorded on each day of observation.
Total body length/Mean dry weight of parental daphnids: At the end of the test, the total length excluding the anal spine of each survived parental daphnid and the mean dry weight of the survived parental daphnids of all the test concentrations and the control were determined. As stated in the OECD 211 protocol, this may be useful additional information but is not used for determination of a NOEC.
Additional endpoints: The time to the first brood, the intrinsic rate of population increase and the number and size of the first brood per animal were reported, but not used for endpoint calculations.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: A non-GLP preliminary range finding test was conducted at the test facility as a shortened reproduction test under semi-static conditions with renewal of the test solutions every 2 – 3 days over a period of 12 days. A control and three test concentrations of the test item of 0.800, 8.00 and 80.0 mg/L were tested in the range finding test. The preliminary range finding test was conducted under diffuse light conditions (light intensity of max. 1500 lx, 16/8 hours light/dark cycle). Five daphnids, one daphnia per replicate, were exposed to each concentration level and the control. The concentration levels and the control were analytically verified via LC-MS/MS at the start of the exposure and in the old media (test vessels containing daphnia and algae) at the end of an exposure-renewal interval
- Test concentrations: Five concentrations were tested in a geometric series with a dilution factor of 3.2: 0.954 - 3.05 - 9.77 - 31.3 - 100 mg/L.
- Results used to determine the conditions for the definitive study: The concentration levels and the control were analytically verified via LC-MS/MS at the start of the exposure and in the old media (test vessels containing daphnia and algae) at the end of an exposure-renewal interval - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC10
- Effect conc.:
- 21.9 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Details on results:
- See overall remarks
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effects on reproduction were evaluated based on the reproduction per introduced parent animal, since this parameter is the ecologically most relevant response variable and is required by the OECD test guideline 211 (2012), when a significant trend in mortality is detected.
The overall effect threshold for effects of the test item under the test conditions was 0.954 mg/L (NOEL) and 3.05 mg/L (LOEL) based on the nominal concentrations of the test item PU-2021-971. The EC10 was 21.9 mg/L based on the nominal concentrations of the test item.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC10
- Effect concentration:
- 21.9 mg/L
Additional information
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