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EC number: 442-680-5 | CAS number: 443688-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 12th to 23rd, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Red LF 6339
- IUPAC Name:
- Red LF 6339
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- samples were taken just before test start (duplicate samples from the single test medium and duplicate samples from the control)
after 48 and 96 hours - stability samples (duplicate samples from the single test medium and duplicate samples from the control)
all samples were taken from the approximate center of the aquaria without mixing of the test media.
Test solutions
- Details on test solutions:
- Stock solution of nominal 500 mg/l was prepared by dissolvig 1.501 g of the test item completely in 3 liters test water by sttirring for 10 min at RT.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Length at study initiation (length definition, mean, range and SD): 4.50 cm
- Weight at study initiation (mean and range, SD): 0.77 g
- Method of breeding: obtained from a trout breeding station Zeiningen, CH-4314, Switzerland
ACCLIMATION
- Acclimation period: more than 2 weeks
- Type and amount of food during acclimation: commercial fish diet
- Feeding frequency during acclimation: yes. until one day before the start of the test
- Health during acclimation (any mortality observed): mortality rate < 5 %. all fish were healthy
FEEDING DURING TEST: not fed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/l as CaCO3
- Test temperature:
- 14 °C
- pH:
- 7.7-7.9
- Dissolved oxygen:
- > 9.2 mg/l
- Nominal and measured concentrations:
- 100 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquarium with 1 liters test medium
- Aeration: slightly aerated during test. test water aerated prior to the preparation of the test medium until oxygen saturation was reached.
- No. of organisms per vessel: 7 fish
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- Biomass loading rate:< 1 g fish wet weight/litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4/1
- Intervals of water quality measurement: at the start of the test and once every day during the test.
OTHER TEST CONDITIONS
- Photoperiod: 16-hr light, 8-hr darkness. 30-minute transition period
- Light intensity: 50-500 lux
EFFECT PARAMETERS MEASURED: after 3, 24, 48, 72 and 96 hours for mortaliry and visible abnormalities. Due to an intense coloration of the test medium by the test item, a 500-W halogen lamp was used to determine the survival and visible abnormalities at the test fish during the test period in the test concentration of nominal 100 mg/l.
TEST CONCENTRATIONS
- Test concentrations: 100 mg/l - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- the test item is sufficiently stable in the test water under test conditions.
analytically determined mean test item concentration in the test medium varied from 93-108 % of the nominal value.
mean measured test item concentration corresponded to 102 % of the nominal value.
no visible abnormalities were observed at the test fish.
the single test solution was a clear solution colored dark-red .
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- LC50 (96 h) > 100 mg/l (nominal)
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