Carbonic acid, 4-nitrophenyl 5-thiazolylmethyl ester, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 Jul - 04 Aug, 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under OECD method and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

414-490-2

EC Name:

-

Cas Number:

154212-59-6

Molecular formula:

C11H9ClN2O5S

IUPAC Name:

4-nitrophenyl (1,3-thiazol-5-yl)methyl carbonate hydrochloride

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The test and std substances were dispersed directly in nutrient medium to give the foloowing series:
a) Inoculated nutrient medium
b) 5mg/l test solution
c) 3mg/l sodium benzoate
d) 5mg/l test solution + 3mg/l sodium benzoate.

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

280ml BOD bottles were incubated in a water bath at 21±1°C and oxygen detrmination performed at 0, 4, 7, 11, 14, 18, 21, 25 and 28 days.

Reference substance

Reference substance:

other: Sodium benzoate

Results and discussion

Preliminary study:

A-87439.1 attained 27% degredation after 28 days

Test performance:

Sodium benzoate attained 72% biodegredation within 28 days.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

27

Details on results:

Nitrate and nitrite analyses were conducted on day 0 and day 28.

Points of degradation plot (test substance):
8 % degradation after 4 d
13 % degradation after 7 d
11 % degradation after 11 d
9 % degradation after 14 d
20 % degradation after 18 d
35 % degradation after 21 d
29 % degradation after 25 d
27 % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
70 % degradation after 4 d
70 % degradation after 7 d
72 % degradation after 11 d
65 % degradation after 14 d
67 % degradation after 18 d
71 % degradation after 21 d
64 % degradation after 25 d
72 % degradation after 28 d

Any other information on results incl. tables

Oxygen depletion levels in the inoculated control series were within the prescribed limits of ≤1.5mg O2/l after 28 days.

Cultures containing both test and standard substances combined showed an oxygen depletion value 4% lower than that anticipated on the basis of results from separate cultures at day 14. Consequently, A-87439.1 is not considered to have had an inhibitory effect on sewage bacteria under the conditions of the test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradble

Conclusions:

The test material attained 27% degradation after 28 days and therefore cannot be considered to be readily biodegradable.

Executive summary:

After correcting the total oxygen consumption (1.355 mgO2/l) for that attributable to nitrification (1.37mg O2/l) the true oxygen consumption value for carbonaceous biodegredation after 28 days is obtained, i.e. 0%.