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EC number: 200-826-3 | CAS number: 74-97-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 948
Materials and methods
- Principles of method if other than guideline:
- Single gavage and 10 day repeated dose toxicity study.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Bromochloromethane
- EC Number:
- 200-826-3
- EC Name:
- Bromochloromethane
- Cas Number:
- 74-97-5
- Molecular formula:
- CH2BrCl
- IUPAC Name:
- bromo(chloro)methane
- Details on test material:
- - Name of test material (as cited in study report): monochloromonobromomethane
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 20 g
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 50% - Doses:
- Single dose (three series of mice): 500, 3,000, 4,500 mg/kg.
One to ten day repeated dose (a fourth series of mice): 3,000 mg/kg. - No. of animals per sex per dose:
- 225 mice altogether.
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes. Several representative mice from the three series were killed for histologic examination at intervals following the single dose, and several from the fourth series, following each of the one to ten doses. Some of the mice that died were also examined.
Results and discussion
Effect levels
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 4 300 mg/kg bw
- Based on:
- test mat.
- Gross pathology:
- Pathological findings in the liver and kidneys were found in mice given a single dose of 3,000 and 4,500 mg/kg. A slight cardiac fatty degeneration was also found in one mouse given 3,000 mg/kg. Besides, the adrenal glands examined exhibited signs of lipoid deplation of the cortex. In the series of mice given multiple doses of 3,000 mg/kg, the lesions were more frequent, severe and prolonged than after a single dose.
Any other information on results incl. tables
Frequency and severity of changes after a single dose: No significant changes were noted after administration of 500 mg/kg of the test substance. After a single dose of 3,000 and 4,500 mg/kg, fatty degeneration of the liver and the kidney was slight or absent in mice that died three or four hours later, was usually severe in the mice killed twenty-four hours later, but was generally not found in those surviving forty-eight hours or longer. Twelve of fifty mice that died or were killed within forty-eight hours showed subcapsular necrosis of the liver, and 5 showed some hydropic liver cells; slight similar changes were seen in one of five mice killed ninety-six hours after receiving the compound. Eight of 32 mice that were killed or died within twenty-four hours after administration of the compound showed hemoglobin casts in a few renal tubes; 7 of these mice received 4,500 mg/kg, and 1 of the 7 showed epithelial necrosis in a few scattered convulted renal tubules. Slight cardiac fatty degeneration was found in only 1 mouse, which died within twenty-four hours after a dose of 3,000 mg/kg. The few adrenal glands examined showed indications of transient lipoid deplation of the cortex.
Frequency and severity of changes after consecutive daily doses: In the series of mice given multiple doses of 3000 mg/kg, the lesions were more frequent, severe and prolonged than after a single dose. 24 to 48 hours after the initial dose, fatty degeneration occurred in the liver, the kidney and less frequently in the heart. It was slight or absent after eighty hours. Twenty-three of 32 mice that died or were killed within seventy-two hours after the initial dose showed subcapsular necrosis of the liver, 8 showed hydropic degeneration and 8 showed a slight to moderate increase of mononuclear cells periportally. Such changes were infrequent after three days and were not seen in mice killed more than five days after the initial dose. One mouse killed nine days after the initial dose showed a few focal and centrolobular areas of coagulation necrosis of the liver cells. Hemoglobin casts were seen in this series in only 1 animal, which died in forty-eight hours, shortly after the third daily dose. Another mouse, which died twenty-four hours after the sixth daily dose, showed extensive tubular necrosis of the inner cortical zone of the kidney. Slight pneumonitis was found in nearly all groups. Cloudiness of the eyes was observed in only 2 of 11 mice that died six hours after the second dose and in 3 of 8 mice that died shortly after the third daily dose of 3,000 mg/kg. It was found in 2 of 8 mice that died after a second daily dose of 4,500 mg/kg.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The oral LD50 in mice is 4300 mg/kg bw.
- Executive summary:
A single gavage and 10 day repeated dose toxicity study was performed with the test substance bromochloromethane. 50% of bromochloromethane in olive oil was administered by stomach tube to several series of white Swiss mice. Three series of mice were given a single dose of 500, 3,000 and 4,500 mg/kg, respectively, and a fourth series was given 3,000 mg/kg on one to ten consecutive days. The control group received olive oil only. Several representative mice from each series were killed for histologic examination and some of the mice that died were also examined. Fatty degeneration of the liver and kidney, as well as focal necrosis and hydropic degeneration of the liver was observed in the 3,000 and 4,500 groups after single oral dose. Changes were most severe after 24 hours and were reversible in mice surviving 48 hours. No effects were noted at 500 mg/kg. In the series of mice given multiple doses of 3000 mg/kg, the lesions were more frequent, severe and prolonged than after a single dose. The oral LD50 in mice was determined to be 4300 mg/kg bw.
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