Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-501-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-10-15 to 2009-04-01
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Series on Testing and Assessment No.23 (Aquatic Toxicity Testing of Difficult Substances and Mixtures, sec. 3.1.2)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- IUPAC Name:
- Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- Reference substance name:
- Acid Yellow 42: 6375-55-9
- IUPAC Name:
- Acid Yellow 42: 6375-55-9
- Reference substance name:
- Acid Black 2: reaction product of 8005-03-6 and 68510-98-5
- IUPAC Name:
- Acid Black 2: reaction product of 8005-03-6 and 68510-98-5
- Reference substance name:
- Basic Violet 1: 8004-87-3
- IUPAC Name:
- Basic Violet 1: 8004-87-3
- Details on test material:
- - Name of test material: Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- Lot/batch No.: 2008041541
- Expiration date of the lot/batch: 2010-01-31
- Stability under test conditions: see expiry date
- Storage condition of test material: store cool and dry
- Analytical purity: 100 % (96.8 %; UVCB substance)
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.74; 0.39; 0.17; 0.09; 0.04 and 0.02 mg/L
- Sampling method: For the analysis of the test item concentration, samples from the test media of the three highest test concentrations and the
control were taken at the start and at the end of the test. All test medium samples were taken from the approximate centre of the
test vessels after mixing of the test media.
- Sample storage conditions before analysis: The samples were freeze-dried (25-fold concentrated) before analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAF method (OECD Series on Testing and Assessment No. 23: Aquatic Toxicity Testing of Difficult Substances and Mixtures, section 3.1.2
- Controls: in the control, test water was used without addition of the test item
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: National Institute of Public Health, Hungary
- Age at study initiation (mean and range, SD): less than 24 hours old
- Method of breeding: The Daphnia were bred under similar temperature and light conditions as in the test, and in reconstituted water of a similar
quality regarding to pH, components of the main ions and total hardness as the test water used in the test.
The test animals were fed daily with a few mL of centrifuged algae suspension (Pseudokirchneriella subcapitata).
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): Brood Daphnids were maintained in ISO medium under test conditions prior to the start of the test.
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- The hardness (CaCO3) of water was: 142 mg/L.
- Test temperature:
- 19.7 – 20.0 °C
- pH:
- 7.28 - 7.87
- Dissolved oxygen:
- The dissolved oxygen concentration at the end of the test was ≥ 6.3 mg/L in control and test vessels.
- Nominal and measured concentrations:
- The following nominal concentrations were used in the main study: S.S./10; S.S./20; S.S./40; S.S./80; S.S./160; S.S./320.
The corresponding calculated test item concentrations were: 0.74; 0.39; 0.17; 0.09; 0.04 and 0.02 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.042 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % CL
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.036 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % CL
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.02 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.02 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Results with reference substance (positive control):
- - Results with reference substance:
24h-EC50 value: 1.59 mg /L , 95 % confidence limits: 1.29 – 1.96 mg /L
In the test with the reference item the results met the expected toxicity of the Potassium dichromate (the 24h EC50 value of the potassium
dichromate should be within the range 0.6 mg/L to 2.1 mg/L). The results are based on the nominal concentrations. - Reported statistics and error estimates:
- The EC50 were calculated based on statistical determination (Probit Analysis, statistical software program SPSS+).
The EC100, EC0, NOEC were determined directly from the raw data.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 48-hour static acute toxicity test to Daphnia magna with F 46 Black the 48 h EC50 value was 0.036 mg/L and the 48 h NOEC was determined to be 0.02 mg/L, based on calculated concentrations.
- Executive summary:
A 48-hour static acute toxicity test with Daphnia magna according to EU method C.2 and OECD guideline 202 revealed a 48 h EC50 value of 0.036 mg/L and a 48 h NOEC of 0.02 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.