Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-737-4 | CAS number: 1220100-43-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Validated in vitro basal cytotoxicity bioassay intended to estimate rodent oral LD50; GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The basal cytotoxicity of the test substance was evaluated in vitro, using a Neutral Red Uptake Assay in Balb/c 3T3 Mouse Fibroblasts. This is a survival/viability chemosensitivity assay based on the ability of viable cells to incorporate and bind neutral red (NR), a supravital dye. NR is a weak cationic dye that readily penetrates cell membranes by non-ionic diffusion and accumulates in lysosomes. Alterations of the cell surface or the sensitive lysosomal membrane result in a decreased uptake and binding of NR. Correlations between cytototoxicity and rodent median lethal dosages (LD50) have been established from existing databases for a wide range of chemicals.
- GLP compliance:
- yes
- Remarks:
- FDA, EPA, OECD
- Test type:
- other: Basal Cytotoxicity in NRU 3T3 Mouse Fibroblasts
- Limit test:
- no
Test material
- Reference substance name:
- N,N'-bis[2-hydroxy-3-(2,2,3,3- tetrafluoropropoxy)propyl]-N,N,N',N'-tetramethylethane-1,2- diaminium dichloride
- IUPAC Name:
- N,N'-bis[2-hydroxy-3-(2,2,3,3- tetrafluoropropoxy)propyl]-N,N,N',N'-tetramethylethane-1,2- diaminium dichloride
- Reference substance name:
- 1,1,2,2,17,17,18,18-octafluoro-6,13-dihydroxy-8,8,11,11-tetramethyl-4,15-dioxa-8,11-diazaoctadecane-8,11-diium dichloride
- EC Number:
- 700-737-4
- Cas Number:
- 1220100-43-5
- Molecular formula:
- C18H34F8N2O4.2Cl
- IUPAC Name:
- 1,1,2,2,17,17,18,18-octafluoro-6,13-dihydroxy-8,8,11,11-tetramethyl-4,15-dioxa-8,11-diazaoctadecane-8,11-diium dichloride
- Details on test material:
- - Physical state: Solid, white
- Storage condition of test material: Room temperature, protected from light, in a dessicator
Constituent 1
Constituent 2
Test animals
- Species:
- other: Not applicable
- Strain:
- other: Not applicable
Administration / exposure
- Doses:
- Based on the results of a dose range-finding assay, eight concentrations of the test substance ranging from 100,000 µg/mL to 401 µg/mL were chosen for two definitive assays. Sodium Lauryl Sulfate (SLS) was used as a positive control.
Results and discussion
Any other information on results incl. tables
The mean NRU50value obtained from two studies with the test substance was 13,700ug/ml, while that of the SLS positive control was 37.1. The SLS result and other study parameters met established criteria for a valid test. The NRU50value obtained for the test substance is considered indicative of a very low order of acute oral toxicity.
Applicant's summary and conclusion
- Interpretation of results:
- other: Very low order of acute oral toxicity
- Conclusions:
- Based upon the in vitro basal cytotoxicity test using Neutral Red Uptake in Balb/c 3T3 mouse fibroblasts, the test substance was considered to have a very low order of acute oral toxicity, with an estimated rodent oral LD50 of 8840 mg/kg.
- Executive summary:
The basal cytotoxicity of the substance was evaluated in vitro, using a Neutral Red Uptake Assay in Balb/c 3T3 Mouse Fibroblasts. This is a survival/viability chemosensitivity assay based on the ability of viable cells to incorporate and bind neutral red (NR), a supravital dye. NR is a weak cationic dye that readily penetrates cell membranes by non-ionic diffusion and accumulates in lysosomes. Alterations of the cell surface or the sensitive lysosomal membrane result in a decreased uptake and binding of NR. Correlations between cytototoxicity and rodent median lethal dosages (LD50’s) have been established from existing databases for a wide range of chemicals.
Based on the results of a dose range-finding assay, eight concentrations of the substance ranging from 100,000 µg/mL to 401 µg/mL were chosen for two definitive assays. Sodium Lauryl Sulfate (SLS) was used as a positive control.
The mean NRU50value obtained from two studies with the test substance was 13,700ug/ml, while that of the SLS positive control was 37.1.The SLS result and other study parameters met established criteria for a valid test. The NRU50value obtained for the substance is considered indicative of a very low order of acute oral toxicity, with an estimated rodent oral LD50 of 8840 mg/kg.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.