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EC number: 700-908-3 | CAS number: 19444-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Study was conducted for 3 days instead of 5.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
- EC Number:
- 700-908-3
- Cas Number:
- 19444-21-4
- Molecular formula:
- C7H12O3
- IUPAC Name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 2 samples (2 times about 30 ml per concentration and control) were taken at the start and 2 samples (2 times about 30 ml per concentration and control) at the end of exposure. Samples were taken from the freshly prepared test solutions immediately before exposure (separately prepared for analytical) and from two composite test vessels after 72 hours of exposure. All samples were kept at -18 to -22 °C until analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
Control (Blank): Blank: Reconstituted water (OECD-medium)
Vehicle: 3µL DMF (dimethylformamide) in 30ml reconstituted water (OECD-medium)
Test item: 3309.2 mg of CA 2215 A were mixed and made up to 10 ml with DMF. This first stock solution was used to prepare all other stock solutions in DMF. 2.5 ml was mixed and made up to 5.0 ml to prepare stock solution 2; 2.5, 1.25, 0.625, 0.3125 and 0.15625 ml were mixed and made up to 10 ml to prepare stock solutions 3, 4, 5, 6 and 7. The test solutions were prepared by adding 3 µl from these stock solutions to 30 ml reconstituted water (OECD-medium) in the test vessels (before the algae were placed in the test vessels).
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green Algae.
- Strain: Selenastrum capricornutum ATCC 22662 ≡ Pseudokirch Subcapitata SAG 61.81.
- Source (laboratory, culture collection): Collection of algae cultures, Pflanzenphysiologisches Institut, University, Nikolausberger Weg 180, D-3400 Göttingen, Germany.
ACCLIMATION
- Acclimation period: 3 days under test conditions.
The algae culture used was demonstrated to be adequately sensitive.
Study design
- Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 24 °C ± 1°C
- pH:
- Between 7.8 - 8.0 and 7.7 - 7.8 at the start and the end of exposure, respectively.
- Nominal and measured concentrations:
- Based on the results of the range-finding-test, the following nominal concentrations were selected for the definitive test.
32.0, 16.0, 8.0, 4.0, 2.0, 1.0 and 0.5 mg test item/L.
Th emeasured test concentrations were 28.4, 13.7, 7.7, 3.9, 1.9, 1.1 and 0.55 mg/L at the start of exposure and 29.5, 14.0, 7.1, 3.6, 1.7, 0.95 and 0.50 mg/L at the end of the exposure. - Details on test conditions:
TEST SYSTEM
- Test vessel:
100 ml Erlenmeyer flask
- Material, size, headspace, fill volume:
30ml test solution per flask.
- Initial cells density:
10600 cells/L
- Replicates:
Each test concetration was tested in 3 replicates, the blank and vehicle controls in 6.
TEST MEDIUM / WATER PARAMETERS
- Ca/mg ratio:
24 mg/CaCO3/L.
- Conductivity:
124 µS.
- Photoperiod/Light intensity and quality:
Continuous illumination, cold white fluorescent light at 117 µE/m2 (approx 800 lux).
MEASUREMENTS:
Cell densities were measuted at 24, 48 and 72 hours of exposure using CytoFluor II (a Fluorecence Multi-Well PLate Reder).
Temperature was continuosly measured.
The pH of the test solution was measured at 0h nad 72 hours of exposure.
Light intensity was mesured at the start of exposure using a Quantum Radiometer/Photometer.- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: 95 % CL
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: Significant on 5% level
- Details on results:
There was no statistically significant (p>0.05) difference in mean cell density between the control and the vehicle and the treatment groups with concentrations of 0.50, 1.0, 2.0 mg/L. Percent inhibition values on the basis of growth rate were 0.088, 0.00, 0.38 and 3.63 %. A statistically significant (p>0.05) difference in mean cell density was present between control and the treatment groups with concentrations of 4.0, 8.0, 16.0 and 32.0 mg/L. Percent inhibition values on the basis of growth rate were 10.67, 16.35, 30.58 and 49.97 %.
No unusual appearance of the algae cells and no flocculation, adherence to glass walls or aggregation could be observed at the end of exposure.
Applicant's summary and conclusion
- Conclusions:
- ErC50 (0 – 72 h) of CA 2215 A was determined to be 32.0 mg/L based on nominal concentrations.
The NOErC growth inhibition (0 – 72 h) was 2.0 mg/L based on nominal concentrations of CA 2215 A. - Executive summary:
Range-finding tests showed that the RrC50 (72h) will be between 1.0 and 10.0 mg/L. Based on the range-finding test, the following nominal concentrations of CA 2215 A were chosen for the main test: 32.0, 16.0, 8.0, 4.0, 2.0, 1.0 and 0.50 mg/L. DMF was used as solvent, not exceeding 100mg/L and with identical vehicle concentration in all test concentrations. 3309.2 mg of CA 2215 A were mixed and made up to 10 ml with DMF. This first stock solution was used to prepare all other stock solutions in DMF. The test solutions were prepared by adding 3 µl from these stock solutions to 30 ml reconstituted water (OECD-medium) in the test vessels (before the algae were placed in the test vessels). A vehicle with 3 µl DMF in 30 ml reconstituted water (OECD-medium) was used for CA 2215 A. When the algae are placed in the test vessels, the exposure starts (0 h). Exposure of Selenastrum capricornutum was started at an initial cell density of 10600 cells/L after pre-culture period of 3 days.
In the test with CA 2215 A, the temperature was maintained constant at 24 ± 1°C. Continuous illumination with cold white fluorescent light at 117 µE/m2(approx. 8000 lux) was used. The pH of the test solutions was between 7.8 – 8.0 at the start and 7.7 – 7.8 at the end of exposure. The multiplication factor of the algae cell density in the control was 77 with 72 hours. The nominal test concentrations were: 32.0, 16.0, 8.0, 4.0, 2.0, 1.0 and 0.050 mg test item/L.
The measured test concentrations of CA 2215 A were 28.4, 13.7, 7.7, 3.9, 1.9, 1.1 and 0.55 mg/L at the start of exposure.
The total content of CA 2215 A was found within 85.6 – 110.0 % at test begin and 85.0 – 100.0 % at test end.
Following calculations of EC50 and NOEC are based n nominal concentrations.
ErC 50 (0 – 72 h): 32.0 mg/L
95% confidence limit: 28.4 – 37.0 mg/L
Slope: 1.121 ± 0.091
NOEC (0 – 72 h): NOErC : 2.0 mg/L
(significant on 5% level)
ErC50 (0 – 72 h) of CA 2215 A was determined to be 32.0 mg/L based on nominal concentrations.
The NOErC growth inhibition (0 – 72 h) was 2.0 mg/L based on nominal concentrations of CA 2215 A.
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