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EC number: 411-070-0 | CAS number: - KY-ET
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 November 1992 - 16 March 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD and/or EC guidelines and according to GLP principles. Limited information available to verify the composition of the used test substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (1984)
- GLP compliance:
- yes
Test material
- Reference substance name:
- A mixture of: 2,4 -bis(N'-(4-methylphenyl)ureido)toluene; 2,6 -bis(N'-(4-methylphenyl)ureido)toluene
- EC Number:
- 411-070-0
- EC Name:
- A mixture of: 2,4 -bis(N'-(4-methylphenyl)ureido)toluene; 2,6 -bis(N'-(4-methylphenyl)ureido)toluene
- Molecular formula:
- C23H24N4O2
- IUPAC Name:
- 3-(2-methyl-5-{[(4-methylphenyl)carbamoyl]amino}phenyl)-1-(4-methylphenyl)urea
- Details on test material:
- - Name of test material (as cited in study report): KY-ET
- Substance type: light yellow solid
- Physical state: solid
- Stability under test conditions: Stable in water for at least 48 hours
- Storage condition of test material: Room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At t = 0 and t = 48h, duplicate samples of 10 mL were taken from the vehicle control and the solutions containing 10%, 32% and 100% of filtrate. Simultaneously, reserve samples of 25 mL were taken from all test solutions.
- Sample storage conditions before analysis: -20 degrees C
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A supersaturated solution of 1000 mg/L nominal was prepared by stirring for 48h; Cremophor RH40 was added as dispersant. After stirring, the saturated solution was filtered and the filtrate was diluted further with ISO-medium with the dispersant Cremophor RH40 to concentrations of 10, 18, 32, 56 and 100% of the filtrate.
- Controls: blank, Cremophor RH40 100 mg/L
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor RH40
- Concentration of vehicle in test medium (stock solution and final test solution): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): After filtering, clear without substance deposits
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding: new batch with <3d old animals; 4 wk max age; renewal every 7d (half medium); daily feeding with freshwater algae
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L CaC03
- Test temperature:
- 19.5-20.5 degrees C
- pH:
- 7.8-8.1
- Dissolved oxygen:
- 8.1-9.7 mg/L
- Nominal and measured concentrations:
- Blank and vehicle controls: nominal = 0 mg/L; measured = 0 mg/L
Test Substance:
Nominal = 10% of filtrate (100 mg/L); measured t0 = 0.14 mg/L, t48 = 0.083 mg/L; mean concentration = 0.11 mg/L
Nominal = 32%' of filtrate (320 mg/L);measured t0 = 0.46 mg/L, t48 = 0.25 mg/L; mean concentration = 0.36 mg/L
Nominal = 100% (1000mg/L); measured t0 = 1.0 mg/L, t48 = 0.74 mg/L; mean concentration = 0.87 mg/L - Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: glass, 100 mL
- Aeration: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-Q water (reverse osmosis purified tap water)
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : imobilization (including mortality) at 24 and 48 hours
TEST CONCENTRATIONS
The low solubility of test substance meant a supersaturated stock solution of nominal 1000 mg/L was made by stirring the substance for 48 h. The resulting solution was then filtered through a paper filter to give a substance-deposit free filtrate. This filtrate was subsequently diluted with ISO medium containing 100 mg/L of the dispersant Cremophor RH40 which resulted in the following test concentrations:
0% (blank control, 100mg/L Cremophor (vehicle control)), 10%, 18%, 32%, 56%, 100% of filtrate. The amount of test substance was later measured at both t = 0h and t = 48h in the controls, and in the 10%, 32%, and 100% of filtrate test concentrations. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.87 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: In the final test solutions no precipitation was observed.
- No effects were observed at the tested concentrations. The 48h-EC50 value was determined to be higher than 0.87 mg/L, which is the highest test concentration. Since this exceeds the maximum solubility of KY-ET in water (0.002 mg/L), it can be concluded that the 48h-EC50 value is above the maximum solubility of KY-ET in water. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
-48h EC50: >0.32 and < 0.56 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No effects were observed at the tested concentrations. The 48h-EC50 value was determined to be higher than 0.87 mg/L, which is the highest test concentration. Since this exceeds the maximum solubility of KY-ET in water (0.002 mg/L), it can be concluded that the 48h-EC50 value is above the maximum solubility of KY-ET in water.
- Executive summary:
An Acute Immobilization Study was performed with Daphnia magna according to OECD 202. The test was performed using filtrates of a supersaturated solution of KY-ET. Three of the filtrate levels (10%, 32% and 100%) were analyzed for actual KY-ET concentrations. The mean measured concentrations were 0.11, 0.36 and 0.87 mg/L, respectively.
No effects were observed at the tested concentrations. The 48h-EC50 value was determined to be higher than 0.87 mg/L, which is the highest test concentration. Since this exceeds the maximum solubility of KY-ET in water (0.002 mg/L), it can be concluded that the 48h-EC50 value is above the maximum solubility of KY-ET in water.
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