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EC number: 691-061-8 | CAS number: 1001320-38-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 July - 7 August 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Also in accordance with GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (2004)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO International Standard 6341: "Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test, Third edition (1996)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23 (2000)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- butyl N-[(butylsulfanyl)methanethioyl]carbamate
- EC Number:
- 691-061-8
- Cas Number:
- 1001320-38-2
- Molecular formula:
- C10H19NO2S2
- IUPAC Name:
- butyl N-[(butylsulfanyl)methanethioyl]carbamate
- Test material form:
- solid
- Remarks:
- yellow grease-like solid
Constituent 1
- Specific details on test material used for the study:
- Molecular formula: C10H19NO2S2
Name: n-butoxycarbonyl n-butyl dithiocarbamate (active ingredient)
CAS Number: 1001320-38-2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations
Combined limit/range finding test: 1, 10 and 100 mg/L water accommodated fractions (WAFs)
Final test: blank control, 0.46, 1.0, 2.2, 4.6 and 10 mg/L WAFs
- Sampling method
Frequency: at t=0 h and t=48 h
Volume: 2.0 mL
- Sample storage conditions before analysis: Samples not analysed on the day of sampling were stored in a freezer until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was warmed to approximately 28°C in order to melt. The liquid test substance was used for weighing out the required amounts of test substance. All loading rates were prepared separately applying one day of magnetic stirring to reach maximum solubility of the test substance in the test medium. The resulting aqueous mixtures were left to stabilize for 30-60 minutes where after the Water Accommodated Fractions (WAFs) were taken out by siphoning. No correction was made for the purity/composition of the test substance.
- Controls: Test medium without test substance or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Test concentrations prepared for the combined limit range-finding test were observed to range from clear and colourless to slightly hazy with a floating layer with increasing loading rate. Test concentrations prepared for the final test were observed to be clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In-house laboratory culture with a known history
- Age at study initiation: <24 h
- Feeding during test: None
BREEDING:
- Method: At least third generation obtained by acyclical parthenogenesis under specified breeding conditions. Each batch started with newborn daphnids (i.e. less than three days old) and the maximum age of the cultures was 4 weeks
- Conditions (same as test or not): yes
- Type of food: suspension of fresh water algae
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- The temperature of the test medium ranged between 20.2 and 21.1°C at the start of the test. The temperature continuously measured in a temperature control vessel varied between 19.3 and 20.0°C during the test.
- pH:
- Start: 7.8-7.9
End: 7.7-7.8 - Dissolved oxygen:
- Start: 8.3-8.8 mg/L
End: 8.4-8.6 mg/L - Nominal and measured concentrations:
- Nominal: Blank control, WAFs prepared at loading rates of 0.46, 1.0, 2.2, 4.6 and 10 mg/L
- Details on test conditions:
- TEST SYSTEM (final test)
- Test vessel: all glass with 100 mL capacity containing 80 mL of test solution. Vessels were fitted with a mesh just under the medium surface to separate the dahnids from possible floating layers.
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading: 5 daphnids per 80 mL of test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by Reverse Osmosis
- Culture medium different from test medium: Yes (M7 medium prepared with adjusted ISO medium)
- Test medium: adjusted ISO medium
- Intervals of water quality measurement: pH was measured at the beginning of the test and at the end for the control and all test concentrations. Dissolved oxygen was measured at the beginning and at the end of the test for the control and all test concentrations. Temperature was measured continuously in a temperature control vessel.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility (including mortality) at 24 and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately x2
- Range finding study
- Test concentrations: blank control and WAFs prepared at a loading rate of 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.42 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CL: 0.37-0.50 mg/L)
- Details on results:
- - Combined limit/range-finding test: After 24 hours of exposure all daphnids exposed to the WAFs prepared at 10 and 100 mg/L were immobilised. At the end of the test 60% of the daphnids exposed to the WAFs prepared at 1.0 mg/L were immobilised as well.
- Final test
Measured concentrations: Concentrations did not remain stable during the test period, i.e. they decreased to 46-74% of initial at the end of the test. Consequently, effect parameters were based on mean exposure concentrations.
Biological results: After 48 hours of exposure no immobility was observed at a mean concentration of 0.23 mg/L, while 90% or more immobilisation was observed at mean concentrations of 0.61 mg/L and higher. - Results with reference substance (positive control):
- - Potassium dichromate: The 48h-EC50 was 0.30 mg/L (95% confidence level between 0.26 and 0.34 mg/L). The sensitivity of this batch of D. magna was within the range of the historical data collected at WIL Research Europe.
Any other information on results incl. tables
Measured concentrations versus loading rates | |||
Loading rate1S-10903 (mg/L) | Measured concentration (mg/L) | Mean conc. (mg/L) | |
t=0 h | t=48 h | ||
0.46 | 0.337 | 0.154 | 0.23 |
1 | 0.806 | 0.458 | 0.61 |
2.2 | 2.02 | 1.3 | 1.6 |
4.6 | 3.14 | 1.97 | 2.5 |
10 | 7.45 | 5.51 | 6.4 |
1WAF prepared at the given loading rate |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 was 0.42 mg/L (95% confidence level between 0.37 and 0.50 mg/L).
- Executive summary:
In a 48-h acute toxicity study conducted according to OECD guideline 202 and GLP principles, water fleas (Daphia magna) were exposed to the test material under static conditions at the following nominal concentrations (final test): 0 (blank-control), WAFs prepared at loading rates of 0.46, 1.0, 2.2, 4.6 and 10 mg/L. The 48h-EC50 was 0.42 mg/L based on average exposure concentrations (95% confidence level between 0.37 and 0.50 mg/L). The study is considered to be reliable without restrictions.
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