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EC number: 287-574-8 | CAS number: 85536-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1981-11-13 to 1981-11-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- RICHTLINIE 88/302/EWG (TEIL C), EG AMTSBLATT L 133/118 "BELEBTSCHLAMM: PRÜFUNG DER ATMUNGSHEMMUNG"
- Deviations:
- yes
- Remarks:
- Test volume 200 mL instead of 500 mL
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Concentrations: Stock solution 2 g/L
- Method: The test solution concentrations were performed dy dilution of the stock solution (Test concentrations: 500 and 1000 ppm (mg/L))
- Controls: blank control perfomed with the test solution without test substance and positive control performed with the reference substance 3,5 dichlorophenol.
- Evidence of undissolved material: no - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: Municipal wastewater treatment plant in Frankfurt-Sindlingen
- Initial biomass concentration: Sludge Dry weight: 100 g of wet sludge in a 4.47 L (22.37 g/L) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 1 °C
- pH:
- 7
- Salinity:
- -
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control) 500 and 1000 mg/L
Measured concentrations: No concentrations were measured.
Positive control: Nominal concentrations:
5, 20 , 30 mg/L - Details on test conditions:
- TEST SYSTEM
- Type: closed
- Aeration: no
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: performed as follows:
90 mL of standard mud
+ 6.5 mL of synthetic wastewater
+ 3.5 mL of distilled water.
+ 100 mL of diluted test - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: >> 1000
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: >> 1000
- Details on results:
- The toxicity of Reactive Orange 72/78 on bacteria ("bacterial toxicity") was investigated in the oxygen-consumption inhibition test ("Robra test") with activated sludge as inoculum. After 3 hours the NOEC and the EC50 were determined to be greater than 1000 mg/L.
- Results with reference substance (positive control):
- The EC50 - value of the reference substance was determined to be < 20 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition rate of oxygen consumption after 3 hours of exposure of bacteria to the test item gave the following results:
EC50: >> 1000 mg/L , NOEC (EC20): >1000 mg/L , EC 80: >> 1000 mg/L - Executive summary:
The toxicity of Reactive Orange 72/78 on bacteria ("bacterial toxicity") was investigated in the oxygen-consumption inhibition test ("Robra test") with activated sludge from municipal wastewater biological treatment Frankfurt / M - Sindlingen as inoculum. The provision of the inhibition rate of oxygen consumption after 3 hours of exposure to the test item on bacteria test gave the following results:
Median inhibitory concentration, EC50: >> 1000 mg/L
NOEC, EC20 : >1000 mg/L
EC 80: >> 1000 mg/L
Reference
Table 1: Inhibition of the respiration rate
mg/L | Activity (mg O2/L x h) | respiration/inhibition | mean |
control 1 | 39 | - | 40.5 |
control 2 | 42 | - | 40.5 |
control 3 | 40.5 | - | 40.5 |
positive control (5) | 24 | 40.7 | - |
positive control (20) | 15 | 63 | - |
positive control (30) | 13.5 | 66.7 | - |
1000 (A) | 43.5 | -7.4 | -9.3 |
1000 (B) | 45 | -11.1 | -9.3 |
500 (A) | 42 | -3.7 | -1.9 |
500 (B) | 40.5 | 0 | -1.9 |
Validity criteria:
The audit was completed without problems, the EC50 - value of the reference substance was 5 to 30 mg / L. The deviation of the controls remained below the limit of 15%.
Description of key information
After 3 hours the EC 50 was determined to be greater than 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The toxicity of Reactive Orange 72/78 on bacteria ("bacterial toxicity") was investigated in the oxygen-consumption inhibition test ("Robra test") with activated sludge from municipal wastewater biological treatment Frankfurt / M - Sindlingen as inoculum. The provision of the inhibition rate of oxygen consumption after 3 hours of exposure to the test item on bacteria test gave the following results:
Median inhibitory concentration, EC50: >> 1000 mg/L
NOEC, EC20 : >1000 mg/L
EC80: >> 1000 mg/L
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