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EC number: 682-238-0 | CAS number: 1190931-27-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-04-27 until 2009-10-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Guideline-conform study under GLP without deviations.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss Federal Office of Public Health, 2008-11-12
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/l of dry salt of test item cC604 (i.e. 625 mg/l of test item cC604, which contains 16% active ingredient)
- Sampling method: Duplicate samples were taken from each treatment before the start of the test and at the end of the test after 48 hours.
- Sample storage conditions before analysis: All samples were stored deep-frozen (at about -20 °C) immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test medium was prepared by mixing 156.3 mg of the test item cC6O4 into 250 mL of test water using ultrasonic treatment for 15 minutes and intense stirring for 15 minutes at room temperature. The test medium was prepared just before introduction of the daphnids (i.e., start of the test). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water fleas
- Strain: Daphnia magna Straus.
- Source: A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield / UK in 1992. Since that time, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests and under temperature and light conditions identical to those of the tests.
- Age at study initiation (mean and range, SD): 6-24 hours
- Weight at study initiation (mean and range, SD): not applicable
- Length at study initiation (length definition, mean, range and SD): not applicable
- Valve height at study initiation, for shell deposition study (mean and range, SD): not applicable
- Peripheral shell growth removed prior to test initiation: not applicable
- Method of breeding: see "source"
- Feeding during test: The daphnids were not fed during the test.
ACCLIMATION
not applicable, daphnids are being bred in the testing laboratory
- Acclimation conditions (same as test or not): not applicable, daphnids are being bred in the testing laboratory
QUARANTINE (wild caught)
not applicable, daphnids are being bred in the testing laboratory - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not reported
- Hardness:
- 2.5 mmol as CaCO3
- Test temperature:
- 20 °C
- pH:
- between 8.0 and 8.1
- Dissolved oxygen:
- 8.3 mg/L
- Salinity:
- No data, however detailed description of the mineral composition of the test medium is given in the study report.
- Nominal and measured concentrations:
- Nominal concentration: 625 mg test item cC6O4/L, corresponding to 100 mg dry salt cC6O4/L (test item cC6O4 contains 84% water).
Measured concentration: 105 to 106% of the nominal value. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open; test vessels were covered with glass plates to reduce the loss of water by evaporation and to avoid the entry of dust into the solutions.
- Material, size, headspace, fill volume: 100-mL glass beakers filled with 50 mL of test medium
- Aeration: The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted test water according to ISO 6341 was used in the study.
- Total organic carbon: not applicable
- Particulate matter: not applicable
- Metals: not applicable
- Pesticides: not applicable
- Chlorine: not applicable
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: No data, however detailed description of the mineral composition of the test medium is given in the study report.
- Culture medium different from test medium: no
- Intervals of water quality measurement: samples taken at the start and at the end of the test after 48 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16-hour light to 8-hour dark cycle with a 30-minute transition period
- Light intensity: between 520 and 680 Lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The daphnids were observed for immobility after 24 and 48 hours of exposure (daphnids not being able to swim within 15 seconds after gentle agitation of the test beaker are considered to be immobilized).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Justification for using less concentrations than requested by guideline: A limit test was performed in accordance with the test guidelines to demonstrate that the test item cC6O4 has no toxic effect on the test organisms up to the nominal concentration of 625 mg test item cC6O4/L, corresponding to 100 mg dry salt cC6O4/L (test item cC6O4 contains 84% water). Thus, a single nominal concentration of the test item cC6O4 of 625 mg/L was tested.
- Range finding study: not applicable - Reference substance (positive control):
- yes
- Remarks:
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: none reported
- Observations on body length and weight: none reported
- Other biological observations: none reported
- Mortality of control: no mortalities reproted
- Other adverse effects control: none reported
- Abnormal responses: none reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none reported
- Effect concentrations exceeding solubility of substance in test medium: none reported - Results with reference substance (positive control):
- - Results with reference substance valid? yes
The result of the latest positive control test in February 2009 (48-hour EC50: 0.71 mg/L) indicated that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from 1996 to 2009: 0.53-1.1 mg/L). - Reported statistics and error estimates:
- no statistics applied
- Validity criteria fulfilled:
- yes
- Remarks:
- no immobilized test otganisms in the controls; positive controls valid
- Conclusions:
- The 48-hour NOEC and EC0 of the test item cC6O4 ammonium salt to Daphnia magna were determined to be at least 100 mg dry salt cC6O4/L.
- Executive summary:
The acute toxicity of the test item cC6O4 ammonium salt to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2, Commission Regulation (EC) No 440/2008, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004). A limit test was performed in accordance with the guidelines to demonstrate that the test item cC6O4 ammonium salt has no toxic effect on the test organisms at a nominal concentration of 625 mg test item cC6O4 ammonium salt/L corresponding to 100 mg dry salt cC6O4 ammonium salt/L (test item aqueous solution of cC6O4 ammonium salt contains 84% water). Thus, a single nominal concentration of the test item cC6O4 ammonium salt was tested. In the control and at the test item cC6O4 ammonium salt concentration of 100 mg dry salt cC6O4/L, no immobilized test organisms were observed during the test period of 48 hours. The summary of the biological results (based on nominal test item concentration of cC6O4 ammonium salt expressed as content of dry salt cC6O4 ammonium salt) is as follows:
– 48 -hour EC50: >100
– 48 -hour EC0 and 48 -hour NOEC: ≥100
In conclusion, the test item cC6O4 ammonium salt had no acute toxic effects on Daphnia magna up to the nominal concentration of 100 mg dry salt cC6O4 ammonium salt/L (corresponding to 625 mg test item cC6O4 ammonium salt/L) under the present test conditions.
Reference
Description of key information
The acute toxicity of the test item cC6O4 ammonium salt to Daphnia magna
was determined in a 48-hour static test according to the EU Commission
Directive 92/69/EEC, Part C.2, Commission Regulation (EC) No 440/2008,
Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004).
The study was performed under GLP without deviations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The limit test demonstrates that the test item cC6O4 ammonium salt has no toxic effect on the test organisms at a nominal concentration of 625 mg test item cC6O4 ammonium salt/L corresponding to 100 mg dry salt cC6O4 ammonium salt/L (test item = aqueous solution of cC6O4 ammonium salt containing 84% water).
EC50-48 hours > 100 mg/l (based on active ingredient)
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