Propanenitrile, 3,3'-(dodecylimino)bis-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Guideline Study under GLP. Results based on nominal concentrations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

EC Number:

605-014-6

Cas Number:

1555-62-0

Molecular formula:

C18H33N3

IUPAC Name:

3-[(2-cyanoethyl)(dodecyl)amino]propanenitrile

Test solutions

Details on test solutions:

nominal concentrations: 0 / 10 / 22 / 50 / 100 mg/L
The substance concentration in test water was monitored by TOC analysis.The measured values ​​were in the beginning of the test in the area of 50.5 to 91.2%.
The concentration decreased during the test due to the low solubility to 11.5 - 16.8%.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

mean body length: 2.9cm (2.7-3.0cm/s=±0,11)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.9-22.2°C

pH:

7.7-8.2

Dissolved oxygen:

7.6-9.3 mg/l

Nominal and measured concentrations:

nominal concentrations: 0 / 10 / 22 / 50 / 100 mg/L
The substance concentration in test water was monitored by TOC analysis.The measured values ​​were in the beginning of the test in the area of 50.5 to 91.2%.
The concentration decreased during the test due to the low solubility to 11.5 - 16.8%.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At all test concentration alterations in appearance and behavior were observed. The dead fish were partially dark or discolored and brightened and several had red gills and lockjaw.
LC50 determined by the geometric mean of LC0 and LC100.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

The toxicity to fish of N,N-Di-(2-cyanoethyl)-dodecylnitril was in examined according to OECD203. The LC50 was determined to be LC50= 14.8mg/L

Executive summary:

The toxicity to fish of N,N-Di-(2-cyanoethyl)-dodecylnitril was in examined according to OECD203. The LC50 was determined to be LC50= 14.8mg/L