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EC number: 210-155-8 | CAS number: 608-25-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: other: genetic toxicity in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- other: genetic toxicity in vivo
Test material
- Reference substance name:
- 2-methylresorcinol
- EC Number:
- 210-155-8
- EC Name:
- 2-methylresorcinol
- Cas Number:
- 608-25-3
- Molecular formula:
- C7H8O2
- IUPAC Name:
- 2-methylbenzene-1,3-diol
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
Any other information on results incl. tables
This study was performed to investigate the potential of the substance to induce micronuclei in
polychromatic erythrocytes (PCE) in the bone marrow of the mouse. The test item was
formulated in deionised water, which was also used as vehicle control. The compound was
administered by a single intraperitoneal injection, and bone marrow cells were collected 24
h and 48 h later. Ten animals (5 males, 5 females) per test group were included and at
least 2000 polychromatic erythrocytes (PCEs) per animal were scored for micronuclei. The
ratio between polychromatic and total erythrocytes was determined in the same sample and
reported as the number of PCEs per 2000 erythrocytes. The following dose levels of the test
item were investigated: 24-h preparation interval: 12.5, 25 and 50 mg/kg bw; 48-h
preparation interval: 50 mg/kg bw. As estimated by pre-experiments, 50 mg substance per kg
bw was the highest applicable dose without significant effects on the survival rates, but with
clear signs of toxicity. At a higher dose (75 mg/kg) all treated animals had to be killed after
10 minutes due to the severity of the induced toxic effects.
Results
The number of PCEs was not substantially decreased in the treated animals as compared
with the vehicle control group, indicating that the substance did not exert any cytotoxic effects on
the bone marrow. The test item was, however, bioavailable as suggested by chemical
analysis of the blood of the treated animals. In comparison with the corresponding vehicle
controls, there was reported to be no biologically relevant or statistically significant
enhancement in the frequency of the detected micronuclei at any preparation interval after
administration of the test item and with any dose level used.
It should be noted that in the 24-h sampling time all doses of the substance resulted in about
doubling of the micronucleus frequency of the vehicle controls. The difference to control was
2.4-, 2.1-, and 2.0-fold, at 12.5, 25, and 50 mg/kg, respectively and was of borderline
significance at 12.5 mg/kg (P=0.0658) and at 25 mg/kg (P=0.0816) in the Mann-Whitney
test. No dose-response was, however, evident, and the micronucleus frequencies were in
general low and within historical control range.
Conclusion
Under the experimental conditions reported, the test item did not significantly induce
micronuclei in mouse bone marrow polychromatic erythrocytes. Therefore, the substance was
considered to be negative in the micronucleus assay. Negative and positive controls were in
accordance with the OECD guideline. Dose selection was based on a dose range-finding
assay.
Comment
At the 24-h sampling time, there was a doubling of mean micronucleus frequencies at all
doses used. The differences were not statistically significant, although borderline, the
numbers of micronucleated cells were, in general, low, and there was no dose-response.
Consequently, it is not biologically relevant.
Applicant's summary and conclusion
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