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Diss Factsheets
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EC number: 613-073-4 | CAS number: 6271-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Details on test material:
- - name of the test substance: Nitrobiphenyl (Reg.Nr. 363 986)
- Purity: 95.5 area%
- batch number: 27997/111 Fr.5
- CAS number: 6271-80-3
- date of manufacturing: 1997-03-11
- physical state/appearance: liquid, red-clear
- storage: room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- - Young adult animals with a comparable weight were used.
- Acclimatization for at least 1 week.
- One animal per cage (type: stainless steel wire mesh cages, type DK-III).
- The animals were housed in fully air-conditioned rooms. Central air-conditioning guaranteed a range of 20 - 24°C for temperature and of 30 - 70% for relative humidity. The day/night rhythm was 12 h light and 12 h darkness.
- Individual animal identification using cage cards and group identification by tail marking.
- The animals were offered a standardized animal laboratory diet as well as tap water ad libitum. The animals were given no feed at least 16 hours bef ore administration, but water was available ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- Good solubility in olive oil DAB 10.
Form of administration: solution
Concentrations used: 4 or 40 g/100 ml
Application volume: 5 ml/kg - Doses:
- 200 and 2000 mg/kg
- No. of animals per sex per dose:
- 3 males (200 and 2000 mg/kg); 3 females (200 mg/kg)
- Control animals:
- no
- Details on study design:
- Observation period: 200 mg/kg - 14 days; 2000 mg/kg - 2 days
Individual body weight determination shortly before administration (day 0), weekly thereafter and at the end of the study (before fasting period).
Recording of signs and symptoms several times on the day of administration, at least once each workday for the individual animals.
A check for any dead or moribund animal was made twice each workday and once on Saturdays, Sundays and on public holidays.
Necropsy at the last day of the observation period. Withdrawal of food at least 16 hours before sacrifice with CO2; then necropsy with gross pathology examination. Necropsy of all animals that died before as early as possible.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 200 - <= 2 000 mg/kg bw
- Mortality:
- 200 mg/kg: males and females: no deaths
2000 mg/kg: males 3/3 after 2 days - Clinical signs:
- other: Male and female animals - 200 mg/kg: no symptoms Male animals - 2000 mg/kg: poor general state, dyspnea, apathy, abdominal and lateral position, staggering, ataxia, atonia, paresis, tremor, spastic gait, erythema, piloerection, smeared fur, exsiccosis, ex
- Gross pathology:
- Animals that died:
2000 mg/kg: glandular stomach: erosion/ulcer, few, diameter up to 2 mm, black; jejunum: discolouration, black: 1 male; stomach: hyperemia, moderate; glandular stomach: erosion/ulcer, few, diameter 2 mm, black; Intestine: discolouration, dark red: 1 male; glandular stomach: erosion/ulcer, few, diameter 1 mm, black; intestine: discolouration, black: 1 male; postmortal state moderate: 2 males
Sacrificed animals:
200 mg/kg: organs without particular findings: 3 males, 3 females
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this study, the test substance needs to be classified R22 and acute oral toxicity Cat. 4, according to Directive 67/548/EEC and Regulation (EU) No. 1272/2008, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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