N,N'-1,4-phenylenebis[3-oxobutyramide]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-07-29 to 2005-10-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study acc. international Guidelines without relevant deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-1,4-phenylenebis[3-oxobutyramide]

EC Number:

246-438-8

EC Name:

N,N'-1,4-phenylenebis[3-oxobutyramide]

Cas Number:

24731-73-5

Molecular formula:

C14H16N2O4

IUPAC Name:

3-oxo-N-[4-(3-oxobutanamido)phenyl]butanamide

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: according to OECD 23;
- Eluate:
- Differential loading: no
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution): no data
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), bred in the test facility
- Age at study initiation (mean and range, SD): 2 - 24 h
- Method of breeding: no data
- Feeding during test: no

ACCLIMATION
- Acclimation period: 2 hours prior test start
- Acclimation conditions (same as test or not): dilution water
- Type and amount of food: no data
- Feeding frequency: no data
- Health during acclimation (any mortality observed): no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no

Test conditions

Hardness:

Elendt, modified to hardness according to 160 - 180 mmol CaCO3/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

60.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

Organic carbon

Basis for effect:

behaviour

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

60.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

organic carbon

Basis for effect:

behaviour

Details on results:

- Behavioural abnormalities: no

Results with reference substance (positive control):

- Results with reference substance valid? yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At the saturated solution (DOC 60.3 mg/L) of the test item Diacetessig-p-phenylendiamid no biologically significant effect was determined.

Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution (DOC 60.3 mg/L) as limit concentration of the test item Diacetessig-p-phenylendiamid , was determined according to OECD 202 (2004) and EC Directive 92/69/EC Method C.2 (1992). The limit test was conducted under static conditions over 48 h from 2005-08-10 to 2005-08-12 at DR.U.NOACK-LABORATORIEN, Käthe-Paulus-Straße 1, D-31157 Sarstedt. 20 test organisms were exposed to the limit concentration and control. The limit concentration and control after 0 h (new media) were analytically verified

via DOC analysis according to DIN EN 1484. For details see part 9. A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 1.84 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.