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EC number: 207-866-0 | CAS number: 498-66-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 8,9,10-trinorborn-2-ene
- EC Number:
- 207-866-0
- EC Name:
- 8,9,10-trinorborn-2-ene
- Cas Number:
- 498-66-8
- Molecular formula:
- C7H10
- IUPAC Name:
- Bicyclo-[2.2.1]-hept-2-ene
- Details on test material:
- lot no 280694
purity 99.8% (a/a)
appearance: white solid
storage conditions: dark at approximately 5°C
stability under test conditions: stable for 4 hours
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98; TA 100; TA1535; TA1537
- Additional strain / cell type characteristics:
- other: all strains: rfa-cell wall deficiency; uvrB-deficient DNA excision-repair system. TA98 and TA 100 strains: presence of pKM101 plasmid (carying r-fctor) increased sensitivity to some mutagens
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix from livers of Arcolor 1254induced (500 mg/kg b.w.) male Sprague-Dawley rats
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500, 2500, 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: without S9: Sodium azide (TA 100, TA1535); 9-Aminoacridine (TA1537; 2-Nitrofluorene (TA98); with S9-mix: 2-Aminoanthracene
- Details on test system and experimental conditions:
- method of application: agar plate incorporation
duration: 48h,
temperature: 37°C,
other environmental conditions: in the dark
evaluation: visual thinning of the bacterial lawn and reduced rate of spontaneously occuring colonies determined through microscopical evalation - Evaluation criteria:
- a) test produces at least 2-fold increase in the mean number of revertants per plate of at least one of the tester strains compared to the appropriate control
b) test induces a dose related increase in the mean number of revertants per plate of at least one of the tester strains compared to the appropirate control in at least 2 to 3 concentrations.
test results must be reproducible
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98; TA 100; TA1535; TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- observed at 2500 and 5000 µg/plate with TA100
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
table presenting the aerage revertant counts for all tests is giving in attachments
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Norbornene was not mutagenic in Salmonella typhimurium (TA98; TA 100; TA1535; TA1537) with and without metabolic activiation (Ames test OECD 471) - Executive summary:
Norbornene was tested in a reverse gene mutation assay (Ames test) in bacteria (S. typhimuriumTA98; TA 100; TA1535; TA1537) in concentrations ranging from 0 to 5000 µg/plate in the presence and absense of mammalian metabolic activation (S9 mix induced from rat liver). The study was performed according to OECD 471 under GLP.
there was no evidence of induced mutant colonies over background. Cytotoxicity was obsevered with TA 100 at the highest concentrations of 2500 and 5000 µg/plate.
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