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Diss Factsheets
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EC number: 469-110-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In a bacterial reverse mutation assay (e.g. Ames test), AD-1000 was tested with four strains of Salmonella typhimurium (TA1535, TA1537, TA100, TA98) and in the Escherichia coli WP2uvrA both in the presence and absence of metabolic activation according to OECD 471 and EC B.13/14 test guidelines. In a dose range finding test up to 5000 µg/l was tested, while in the main test up to 1000 µg/l was tested. In both tests precipitation of the substance was observed. AD-1000 did not induce a dose-related two-fold increase in the number of revertant colonies in any of the bacteria tested. The substance is therefore considered not mutagenic in the Ames test.
AD-1000 was tested in a chromosome aberration study with cultured peripheral human lymphocytes in the presence and absence of metabolic activation according to OECD 473 and EC B.10 test guidelines. Two assays were performed, in the first the substance was tested up to 33 µg/ml for a 3 hour exposure time with 24 h fixation time, while in the second test the substance was tested also up to 33 µg/ml but for a 24 hour and 48 hour exposure time with a 24 hour and 48 hour fixation time in the absence of S9 mix and for a 3 hour exposure time with a 48 fixation time in the presence of S9 mix. AD-1000 precipitated in the culture medium. The substance did not induce a statistically significant or biologically relevant increase in the number of cells with chromosome aberrations in the absence or presence of metabolic activation. In addition, the substance did not have any effect on the number of polyploid cells and cells with endoreduplicated chromosomes and thus does not disturb mitotic processes and cell cycle progression. AD-1000 is not clastogenic in this study.
In a TK assay according to OECD 476 and EC B.17 test guidelines, mouse lymphoma L5178Y cells were exposed to AD-1000 with and without metabolic activation. Based on a dose-range finder, the main test consisted of two experiments, the first with 3 hours of exposure to the substance at concentrations up to 100 µg/ml with and without metabolic activation and the second experiment with 24 hours exposure up to 80 µg/ml and only without metabolic activation. Precipitation was observed at the highest concentrations. AD-1000 did not have a genotoxic effect in this assay.
Short description of key information:
In an Ames test, a chromosome aberration test and a TK assay, all according to current test guidelines, no genotoxicity was observed for AD-1000.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Based on the studies available, AD-1000 does not have to be classified according to Directive 67/548/EEC and the CLP Regulation (EC) No 1272/2008 for genetic toxicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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