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EC number: 700-144-0 | CAS number: 13115-71-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-09-15 to 2009-09-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April, 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 31 May, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 6.25, 12.5, 25, 50 and 100 mg a.i./L (after 0 and 24 h first exposure interval and after 0 and 24 h second exposure interval)
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a concentration of 1 g a.i./L was prepared. The required amount of test material was added to the test medium and stirred for 15 minutes. The test concentrations were prepared separately by dilution of the stock solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): < 24 h
- Source: bred at testing facility, originally obtained from "Landesumweltamt (NRW)", Düsseldorf, Germany
- Feeding during test : no
- Method of breeding: Bred in M4 medium at 20 ± 2 °C and a photoperiod of 16:8 h day/night.
- Type and amount of food: algae cultures (Desmodesmus subspicatus) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.4 - 21.3 °C
- pH:
- 7.71 - 8.51
- Dissolved oxygen:
- 4.6 - 5.8 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations. 6.25, 12.5, 25, 50 and 100 mg a.i./L
Recovery rate: 93% and 97% of nominal test concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- Fill volume: 30 mL
- Aeration: no
- Renewal rate of test solution: after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Culture medium different from test medium: same as test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night cycle
- Light intensity: 700 ± 50 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mobility after 24 and 48 hours
RANGE-FINDING STUDY
A range-finding study was performed but the results were not presented in detail. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Immobilisation of control:
no
- Results with reference substance (positive control):
- - Results with reference substance valid?
Yes (conducted 2009-08-05 to 2009-08-06)
- Relevant effect levels: EC50 (24 h): 0.99 mg/L - Reported statistics and error estimates:
- No statistical evaluation was necessary because no effects on daphnia were observed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of this study show that the test substance Glycyl-L-glutamine monohydrate induced no immobilisation to the exposed Daphnia within 48 hours. The EC50 value after 2 days of exposure was >100 mg a.i./L and the NOEC was ≥ 100 mg a.i./L, respectively. As analytical determination showed no relevant loss of test substance during the first and the second exposure interval EC values were based on nominal concentration.
Reference
Description of key information
EC50 (48 h) >100 mg/L (nominal, OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
The acute toxicity of the substance to aquatic invertebrates was investigated in a semi-static test set up according to OECD 202 (GLP). Daphnia magna were expsoed for 48 hours to nominal test concentrations of 6.25, 12.5, 25, 50 and 100 mg a.i./L including a control. The test solutions were renewed daily. An analytical dose verfication of freshly prepared and aged medium was performed (HPLC/UV) and the determined recovery rate ranged from 93% - 97% of nominal. During the 48 hour exposure period no immobilisation of Daphnids was observed and therefore an EC50 (48 h) >100 mg/L was determined.
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