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EC number: 938-347-3 | CAS number: 28068-91-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 April - 28 May 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Protocol for the study was developed in accordance with the OECD guideline on the bacterial reverse mutation test. Study is performed by a GLP accredited laboratory.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of (2R,4R)-4-(2,3-dimethylbutan-2-yl)-2-methylcyclohexanone and (2S,4S)-4-(2,3-dimethylbutan-2-yl)-2-methylcyclohexanone
- EC Number:
- 938-347-3
- Cas Number:
- 28068-91-9
- Molecular formula:
- C13H24O
- IUPAC Name:
- Reaction mass of (2R,4R)-4-(2,3-dimethylbutan-2-yl)-2-methylcyclohexanone and (2S,4S)-4-(2,3-dimethylbutan-2-yl)-2-methylcyclohexanone
- Details on test material:
- - Name of test material (as cited in study report): Iriswood
- Substance type: pure active substance
- Physical state: undercooled liquid
1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- Bacterial strains were exposed to the test system at 0.06, 0.19, 0.56, 1.67, and 5 µl/plate. The lower concentrations were prepared by 1:3 serial
dilutions of the highest concentration. - Vehicle / solvent:
- 96% ethanol
Controls
- Untreated negative controls:
- yes
- Remarks:
- vehicle of test substance
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol 96%
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see details of test system and conditions.
- Details on test system and experimental conditions:
- The bacterial strains used for the study were grown from controlled Working Banks obtained from Master Banks (generated in Vivotecnia) in nutrient broth supplemented with the corresponding antibiotics when required.
Inoculums were liquid grown overnight until the late exponential-early stationary phase of growth was reached (approximately 1.2-1.4 OD at 660nm). This OD indicates that bacteria are growing in the late exponential or early stationary phase of growth (approximately 1-2x109 CFU/mL). - Evaluation criteria:
- The Samonella typhimurium and the Escherichia coli reverse mutation assays are considered acceptable if:
1) the vehicle control is within historical range in each strain.
2) the responses of the positive controls are in historical range. - Statistics:
- The test substance was considered mutagenic if for any strain the total number of revertants in tester strain TA98, TA100 ,WP2(pKM101) is greater than two times the concurrent control and the total number of revertants in tester strains TA1535, TA1537 is greater than three times the concurrent control.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- No cytotoxicity was observed at the maximum test substance concentration (Table 2).
None of the concentrations showed an increase in the colony count (R value) either with or without S9 metabolic activation.
No dose-response for the test substance was observed in any of the bacterial strains tested. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 2 Mean results of the test substance cytotoxicity assay (10 -12 April 2012) with S. typhimurium TA100. The test substance was dissolved in 96% ethanol.
µl/plate | revertants /plate |
s.d. | R |
0 (solvent) | 84 | 5.6 | |
0.06 | 72 | 8.3 | 0.9 |
0.19 | 66 | 1.7 | 0.8 |
0.56 | 68 | 6.4 | 0.8 |
1.67 | 77 | 3.6 | 0.9 |
5.00 | 71 | 5.6 | 0.8 |
Table 2a Mutagenic response of test substance in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli strain. Mean number of revertant colonies per three replicate plate (± standard deviation) without metabolic activation - direct incorporation.
TA98 | TA100 | TA1535 | TA1537 | WP2uvrA | |||||||||||||||
revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | |||||
solvent control | 19 | 5.3 | 90 | 9.0 | 19 | 4.9 | 6 | 3.2 | 239 | 36.8 | |||||||||
positive control | 395 | 44.3 | 20.8 | 826 | 37.7 | 9.1 | 859 | 61.3 | 46.0 | 182 | 17.8 | 28.7 | 1994 | 204.0 | 8.4 | ||||
5 | 16 | 4.2 | 0.9 | 70 | 8.7 | 0.8 | 22 | 8.7 | 1.2 | 7 | 2.5 | 1.2 | 261 | 42.7 | 1.1 | ||||
1.67 | 21 | 8.3 | 1.1 | 82 | 4.6 | 0.9 | 18 | 8.5 | 1.0 | 6 | 2.1 | 1.0 | 301 | 31.5 | 1.3 | ||||
0.56 | 23 | 4.5 | 1.2 | 85 | 10.4 | 0.9 | 20 | 6.1 | 1.1 | 8 | 2.6 | 1.3 | 266 | 40.0 | 1.1 | ||||
0.19 | 18 | 1.7 | 0.9 | 78 | 14.0 | 0.9 | 16 | 3.6 | 0.9 | 7 | 3.8 | 1.1 | 272 | 19.6 | 1.1 | ||||
0.06 | 19 | 5.8 | 1.0 | 88 | 9.0 | 1.0 | 17 | 1.5 | 0.9 | 9 | 1.5 | 1.4 | 275 | 33.7 | 1.2 |
Table 2b Mutagenic response of test substance in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli strain. Mean number of revertant colonies per three replicate plate (± standard deviation) without metabolic activation - pre-incubation.
TA98 | TA100 | TA1535 | TA1537 | WP2uvrA | |||||||||||||||
revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | |||||
solvent control | 22 | 1.7 | 81 | 9.5 | 26 | 2.0 | 5 | 1.5 | 244 | 37.2 | |||||||||
positive control | 335 | 21.2 | 15.2 | 877 | 40.1 | 10.8 | 910 | 51.9 | 35.0 | 170 | 18.6 | 31.9 | 2162 | 121.7 | 8.9 | ||||
5 | 21 | 2.3 | 1.0 | 77 | 3.1 | 0.9 | 14 | 2.3 | 0.5 | 8 | 0.6 | 1.4 | 260 | 42.7 | 1.1 | ||||
1.67 | 20 | 4.7 | 0.9 | 69 | 6.6 | 0.9 | 15 | 1.5 | 0.6 | 4 | 0.6 | 0.8 | 310 | 38.1 | 1.3 | ||||
0.56 | 17 | 6.7 | 0.8 | 75 | 11.4 | 0.9 | 17 | 3.5 | 0.7 | 9 | 3.5 | 1.6 | 296 | 20.4 | 1.2 | ||||
0.19 | 19 | 5.7 | 0.8 | 78 | 7.4 | 1.0 | 23 | 7.5 | 0.9 | 4 | 0.6 | 0.8 | 280 | 17.5 | 1.1 | ||||
0.06 | 20 | 2.1 | 0.9 | 78 | 14.0 | 1.0 | 16 | 3.5 | 0.6 | 6 | 2.1 | 1.1 | 238 | 24.7 | 1.0 |
Table 3a Mutagenic response of test substance in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli strain. Mean number of revertant colonies per three replicate plate (± standard deviation) with metabolic activation - direct incorporation.
TA98 | TA100 | TA1535 | TA1537 | WP2uvrA | |||||||||||||||
revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | |||||
solvent control | 18 | 2.6 | 73 | 5.7 | 19 | 4.0 | 7 | 2.3 | 265 | 19.4 | |||||||||
positive control | 727 | 19.7 | 40.4 | 1751 | 79.8 | 23.9 | 354 | 41.2 | 18.3 | 208 | 15.3 | 31.2 | 1952 | 16.9 | 7.4 | ||||
5 | 15 | 2.5 | 0.9 | 73 | 4.5 | 1.0 | 19 | 0.0 | 1.0 | 8 | 3.5 | 1.2 | 254 | 62.6 | 1.0 | ||||
1.67 | 22 | 2.1 | 1.2 | 65 | 9.7 | 0.9 | 18 | 2.5 | 0.9 | 6 | 1.2 | 1.0 | 267 | 16.7 | 1.0 | ||||
0.56 | 22 | 2.5 | 1.2 | 70 | 7.5 | 1.0 | 20 | 3.5 | 1.1 | 5 | 1.2 | 0.8 | 217 | 37.4 | 0.8 | ||||
0.19 | 27 | 5.0 | 1.5 | 74 | 5.9 | 1.0 | 17 | 2.0 | 0.9 | 6 | 0.6 | 0.9 | 260 | 35.0 | 1.0 | ||||
0.06 | 19 | 3.2 | 1.1 | 80 | 20.5 | 1.1 | 15 | 3.2 | 0.8 | 6 | 1.2 | 1.0 | 256 | 31.7 | 1.0 |
Table 3b Mutagenic response of test substance in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli strain. Mean number of revertant colonies per three replicate plate (± standard deviation) without metabolic activation - pre-incubation.
TA98 | TA100 | TA1535 | TA1537 | WP2uvrA | |||||||||||||||
revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | revertants / plate |
SD | R | |||||
solvent control | 23 | 3.2 | 82 | 18.2 | 20 | 7.0 | 6 | 4.5 | 238 | 28.0 | |||||||||
positive control | 615 | 23.9 | 26.3 | 1477 | 56.0 | 18.1 | 455 | 66.2 | 22.8 | 167 | 10.7 | 26.3 | 2164 | 131.9 | 9.1 | ||||
5 | 18 | 6.0 | 0.8 | 106 | 12.4 | 1.3 | 21 | 7.2 | 1.1 | 6 | 2.0 | 0.9 | 260 | 29.4 | 1.1 | ||||
1.67 | 17 | 1.2 | 0.7 | 89 | 13.0 | 1.1 | 20 | 6.1 | 1.0 | 8 | 4.6 | 1.3 | 296 | 50.5 | 1.2 | ||||
0.56 | 16 | 5.9 | 0.7 | 76 | 4.0 | 0.9 | 18 | 6.8 | 0.9 | 7 | 1.5 | 1.2 | 297 | 23.1 | 1.2 | ||||
0.19 | 16 | 1.2 | 0.7 | 75 | 3.8 | 0.9 | 20 | 3.1 | 1.0 | 6 | 1.0 | 0.9 | 272 | 28.0 | 1.1 | ||||
0.06 | 18 | 3.8 | 0.8 | 100 | 7.5 | 1.2 | 17 | 6.2 | 0.9 | 9 | 2.0 | 1.4 | 274 | 16.1 | 1.1 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
negative with metabolic activation
Based on the results, it is concluded that the test substance is not mutagenic in the Salmonella typhimurium and the Escherichia coli reverse mutation assay. - Executive summary:
The bacterial reverse mutation test (Ames test) assesses the mutagenic or promutagenic potential of the test substance in the several bacterial strains. The test was performed in accordance with OECD Guideline 471. No cytotoxic activity was observed at a test item concentration of 5μL/plate. Five test substance doses ranging from 5.00 and 0.06 μL/plate were assayed. None of the concentrations showed an increase in the colony counting either with or without S9 metabolic activation regardless of the exposure procedure. No dose response for the test substance was observed in any of the tested bacterial strains. Based on the results obtained in this study, it can be concluded that the test item does not induce point mutations or frame-shifts in the genome of the bacterial strains with or without metabolic activation regardless of the procedure. Therefore, the test substance is considered to be NON MUTAGENIC / NON PRO-MUTAGENIC under the experimental conditions assayed.
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