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Diss Factsheets
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EC number: 924-751-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The microbial inoculum was obtained on 22 June 2022 from the activated sludge of the biological urban wastewater treatment plant of Conselve (Padova, Italy). The microbial cells count at test start was 2.6 × 109 CFU/L. The suspended solids of the sludge were 5,130 mg/L, so it was diluted to have 28 mg/L of suspended solids in the of final reaction medium (volume of the reaction vessel: 250 mL). According to the OECD test guidelines, the concentration of suspended solids in the reaction medium must be < 30 mg/L and microbial cells should be 107-108 CFU/L.
Before being used for test, the inoculum was conditioned by aerating it for 5 days at the test temperature. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 27.2 mg/L
- Based on:
- ThOD
- Remarks:
- 78 mgO2/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- BOD
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 47.4
- Sampling time:
- 10 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67.9
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on results of the present study, the maximum average degradation percentage of the test substance is 67.9% (after 28 days), thus the test substance can be deemed as rapidly (readily) biodegradable.
- Executive summary:
The method used during the test is the OECD/OECD 301:1992 “Ready biodegradability” (Test F, Manometric Respirometry Test).
A measured volume of inoculated mineral medium, containing a known concentration of test substance (e.g. 100 mg test substance/l giving at least 50-100 mg ThOD/L) as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (± 1°C) for up to 28 days. The continuous stirring creates a suspension of the sample which is acceptable for water-insoluble samples (§ 4, OECD 301:1992).
The consumption of oxygen is determined by measuring the decrease of partial pressure due to the consumption of O2, with a sensor hosted in the cap of the vessel. Evolved carbon dioxide does not add to the pressure in the headspace of the vessel, since it is absorbed by a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of the ThOD (Theoretical Oxygen Demand) calculated stoichiometrically or COD (Chemical Oxygen Demand) measured in the laboratory. Whenever possible ThOD should be preferred over COD (§§ 2 and 22, OECD 301:1992).
PASS LEVEL: to define a readily biodegradable substance, the pass level for biodegradability is 60% with reference to ThOD or COD.The “REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006” reports the evaluation criteria for the rapid degradability of organic substances at § 4.1.2.9.5:
“Substances are considered rapidly degradable in the environment if one of the following criteria holds true:
(a) if, in 28-day ready biodegradation studies, at least the following levels of degradation are achieved:
(i) tests based on dissolved organic carbon: 70%;
(ii) tests based on oxygen depletion or carbon dioxide generation: 60% of theoretical maximum.
These levels of biodegradation must be achieved within 10 days of the start of degradation which point is taken as the time when 10% of the substance has been degraded, unless the substance is identified as an UVCB or as a complex, multi-constituent substance with structurally similar constituents. In this case, and where there is sufficient justification, the 10-day window condition may be waived and the pass level applied at 28 days. …”.Based on results of the present study, the maximum average degradation percentage of the test substance is 67.9% (after 28 days), thus the test substance can be deemed as rapidly (readily) biodegradable.
Reference
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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