Reaction products of Soya and Linseed Oil Fatty Acids with Bisphenol A diglycidylether

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Biodegradation in water: screening test, toxicity control is used to derive effect concentration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source of test organisms: Activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preperation of inoculum for exposure: The freshly obtained sludge was kept under continuous aeration until further treatment. Before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium. After treatment, the concentration of suspended solids (SS) was determined to be 3 g/L in the concentrated sludge as used for the test. The sludge was used as inoculum at a concentration of 2 mL per litre of mineral medium, leading to a final concentration SS of 5 mg/L.

Test type:

static

Water media type:

freshwater

Total exposure duration:

28 d

Nominal and measured concentrations:

33 mg/L nominal

Details on test conditions:

TEST CONDITIONS
- Composition of the medium: mineral medium as recommended in the OECD Guideline
- Test temperature: 20-22 °C
- pH: 7.7 at test start
- pH adjusted: no
- Light conditions: The test media were excluded from light, brown colored glass bottles were used.

TEST SYSTEM:
- Culturing apparatus: Lovibond BD600-GLP manometric respirometry system equipped with an inductive stirring system.
- Number of flasks: 2 flasks containing test item and inoculum

SAMPLING:
- Sampling was done on day 0-4-7-11-14-18-21-25-28

CONTROL AND BLANK SYSTEM
- 2 flasks containing only inoculum
- 1 flask containing reference item and inoculum
- 1 flask containing inoculum, and test item and reference item in amounts contributing equally to the total ThOD
- 1 flask toxicity control (test item plus sodium acetate).

Reference substance (positive control):

no

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control, the test item was found not to inhibit microbial activity.

Conclusions:

In the toxicity control, the test item was found not to inhibit microbial activity.

Executive summary:

The objective of the study was to evaluate the test item Soya/Linseed Oil Fatty Acid-BADGE reaction product for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge; Manometric Respirometry Test. The study procedures described in this report were in compliance with the OECD guideline No. 301 F, 1992. Within this study, a toxicity control was used to investigate the potential to inhibit microorganisms.

The test item was a clear colourless viscous liquid UVCB. The Theoretical Oxygen Demand (ThOD) of the test item was determined to be 2.38 mg O₂/mg. The test item was tested in duplicate at a target concentration of 33 mg/L, corresponding to a ThOD of 80 mg O₂/L. The ThOD was based on the results of elemental composition analysis.

The study consisted of six bottles:

·      2 inoculum blanks (no test item),

·      2 test bottles (test item),

·      1 procedure control (sodium acetate) and

·      1 toxicity control (test item plus sodium acetate).

In order to transfer test item to the test vessels, the test item was weighed on an inert carrier, which was transferred to the test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was28 days.

The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.

In the toxicity control, the test item was found not to inhibit microbial activity.

One of the criteria for acceptability of the test was not met. However, the Sponsor has indicated that the observed behaviour was consistent with the nature of the test item. Furthermore, both biodegradation values observed would lead to the conclusion that the test item is not readily biodegradable. Therefore, this study was considered to be valid and is reported with a >20% difference between replicate A and B.

In conclusion,Soya/Linseed Oil Fatty Acid-BADGE reaction product was designated as not readily biodegradable.

Description of key information

14 d NOEC = 33 mg/L

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

33 mg/L

Additional information

The objective of the studywas to evaluate the test item Soya/Linseed Oil Fatty Acid-BADGE reaction product for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge;Manometric Respirometry Test.The study procedures described in this report were in compliance with the OECD guideline No. 301 F, 1992. Within this study, a toxicity control was used to investigate the potential to inhibit microorganisms.

The test item was a clear colourless viscous liquid UVCB. The Theoretical Oxygen Demand (ThOD) of the test item was determined to be 2.38 mg O₂/mg. The test item was tested in duplicate at a target concentration of 33 mg/L, corresponding to a ThOD of 80 mg O₂/L. The ThOD was based on the results of elemental composition analysis.

The study consisted of six bottles:

·      2 inoculum blanks (no test item),

·      2 test bottles (test item),

·      1 procedure control (sodium acetate) and

·      1 toxicity control (test item plus sodium acetate).

In order to transfer test item to the test vessels, the test item was weighed on an inert carrier, which was transferred to the test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was28 days.

The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.

In the toxicity control, the test item was found not to inhibit microbial activity.

One of the criteria for acceptability of the test was not met. However, the Sponsor has indicated that the observed behaviour was consistent with the nature of the test item. Furthermore, both biodegradation values observed would lead to the conclusion that the test item is not readily biodegradable. Therefore, this study was considered to be valid and is reported with a >20% difference between replicate A and B.

In conclusion,Soya/Linseed Oil Fatty Acid-BADGE reaction product was designated as not readily biodegradable. The test item was not inhibitory to microorganisms.