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EC number: 401-700-2 | CAS number: 3100-36-5 CYCLOHEXADECENON; CYCLOHEXADECENONE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021-09-08 to 2021-10-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 18.8 - 21.5 °C
- Moisture:
- day 0: 42.9-43.9% of MWHC (Maximum Water Holding Capacity);
day 28: 43.0-46.1% of MWHC (Maximum Water Holding Capacity); - Organic carbon content (% dry weight):
- 0.656
- Nitrogen content (% dry weight):
- 26.1
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Plastic boxes (volume 1.0 L, food grade) with perforated tops to enable gas exchange.
- Amount of soil: 400 g DW
- No. of replicates per concentration: 3
- No. of replicates per control: 3
SOIL INCUBATION
- Method: bulk / series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- A field fresh, silty sand soil (LUFA standard soil 2.3) was used in the study.
- Soil texture: silty sand
- % sand: 57 %
- % silt: 35%
- % clay: 8
- pH (in water): 6.2
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 25.4
- Maximum water holding capacity (in % dry weigth): 35.5
- Cation exchange capacity (meq /100 g): 6.0
- Storage (condition, duration): The soil was stored for 61 days (2021-06-18 to 2021-08-17) in the dark at 4 - 6 °C in a climatic room. Subsequently, the soil was
pre-incubated at room temperature (ca. 20 °C) for 22 days (2021-08-17 to 2021-09-08) to adapt the micro-organisms to test conditions before the start of the study.
- Initial microbial biomass as % of total organic C: 2.22
RANGE-FINDING STUDY
- Test concentrations: 10-100-1000 mg/kg DW
- Results used to determine the conditions for the definitive study: Yes - Nominal and measured concentrations:
- Nominal concentrations: 1000 - 316 - 100 - 31.6 - 10 mg/kg soil dry weight
- Reference substance (positive control):
- yes
- Remarks:
- Cyanoguanidine
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: The Nitrate-N content
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 37 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: The Nitrate-N content
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 286 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: The Nitrate-N content
- Details on results:
- See below
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Validity criteria fulfilled:
- yes
- Remarks:
- Variation between control replicates was < 15 % at days 0, 7, 14 and 28 for the determination of inorganic nitrogen (nitrate-N content).
- Conclusions:
- The toxicity of the test item to soil microorganisms was determined and resulted in an EC10 value of 37 mg/kg DW and EC50 value of 286 mg/kg DW, respectively.
- Executive summary:
The toxicity of the test item to soil microorganisms was determined according to OECD Guideline 216 (2000) and GLP. Based on the result from a preliminary study, the test item was applied via quartz sand at concentrations of 1000 - 316 - 100 - 31.6 - 10 mg/kg soil dry weight (factor √10). Untreated field soil, adjusted to a moisture content corresponding to nominally 45 % of its maximum water holding capacity and mixed with a source of organic nitrogen (lucerne-greengrass-meal), was tested as the control under the same test conditions as the test item replicates. Plastic boxes (volume 1.0 L) with perforated tops to enable gas exchange filled with 400 g soil dry weight were used as test vessels.The effects of the test item on nitrogen transformation by soil microflora were determined by comparing rates of formation of the final transformation product, nitrate-nitrogen (NO3--N), in soil treated with the test item against the rate observed in the untreated control. Under the test conditions, the EC10 and EC50 values were determined to be 37 mg/kg DW and 286 mg/kg DW, respectively.
Reference
Inhibition of Nitrate-N Content in Soil Containing test item, Relative to the Control
Nitrate-N Content | ||||
Test item concentration | Inhibition [%] compared to control | |||
[mg/kg DW] | d 0 | d 7 | d 14 | d 28 |
10 | 4 | 10 | 1 | 1 |
31.6 | 15 | 16 | 17 | 14 |
100 | 12 | 35 | 24 | 21 |
316 | 11 | 100 | 71 | 53 |
1000 | 4 | 100 | 100 | 100 |
Inhibition of Nitrate-N Formation Rates in Soil Containing test item, Relative to the Control:
Nitrate-N Formation Rate | |||
Test item concentration | Inhibition [%] compared to control | ||
[mg/kg DW] | d 0-7 | d 0-14 | d 0-28 |
10 | 35 | -5 | -1 |
31.6 | 19 | 21 | 12 |
100 | n.d. | 54 | 32 |
316 | n.d. | n.d. | n.d. |
1000 | n.d. | n.d. | n.d. |
n.d. = not determinable due to negative formation rates in the replicates
Description of key information
A study on the toxicity of the item to soil microorganisms is available. The test was performed according to OECD Guideline 216 (2000) and GLP. Based on the result from a preliminary study, the test item was applied via quartz sand at concentrations of 1000 - 316 - 100 - 31.6 - 10 mg/kg soil dry weight (factor √10). Untreated field soil, adjusted to a moisture content corresponding to nominally 45 % of its maximum water holding capacity and mixed with a source of organic nitrogen (lucerne-greengrass-meal), was tested as the control under the same test conditions as the test item replicates. Plastic boxes (volume 1.0 L) with perforated tops to enable gas exchange filled with 400 g soil dry weight were used as test vessels.The effects of the test item on nitrogen transformation by soil microflora were determined by comparing rates of formation of the final transformation product, nitrate-nitrogen (NO3--N), in soil treated with the test item against the rate observed in the untreated control. Under the test conditions, the EC10 and EC50 values were determined to be 37 mg/kg DW and 286 mg/kg DW, respectively.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 286 mg/kg soil dw
- Long-term EC10 or NOEC for soil microorganisms:
- 37 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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