Registration Dossier
Registration Dossier
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Diss Factsheets
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EC number: 446-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 - 06 Apr 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Radiolabelling:
- no
- Test temperature:
- Column temperature: 40 °C
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: Kontron HPLC system with autosampler 465, gradient former 425, pump 422 and column oven 480, UV/VIS detector 430; Software Chromeleon Vers. 6.40, Fa. Dionex
- Type, material and dimension of analytical (guard) column: Material: stainless steel (Bischoff)dimensions: 250 x 4.6 mm stationary phase: Zorbax CN, 5 µm column temperature: 40°C
- Detection system: UV/VIS detection system
MOBILE PHASES
- Type: Methanol / water 55/45 (v/v) (isocratic elution)
- Experiments with additives carried out on separate columns: No
- pH: Not specified
- Solutes for dissolving test and reference substances: Direct injection with eluent
DETERMINATION OF DEAD TIME
- Method: by means of homologous series / by inert substances which are not retained by the column: The dead time is determined by using a homologous series of n-alkyl methyl ketones.
REFERENCE SUBSTANCES
- Identity: Acetanilide, Atrazine, 3,5-Dinitrobenzamide, Linuron, Endosulfan-diol, Fenthion, Pyrazophos, Endosulfan, Diclofop-methyl
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 µL injection volume
- Quantity of reference substances: 20 µL injection volume
- Intervals of calibration: Calibration at the start of the test
REPETITIONS
- Number of determinations: 2
EVALUATION
- Calculation of capacity factors k': (tR-t0)/ k' = (capacity factor)
- Calculation of retention times: Retention times were measured
- Determination of the log Koc value: Based on the calibration curve given by the reference substances. - Sample No.:
- #1
- Type:
- Koc
- Value:
- 64
- Temp.:
- 40 °C
- Remarks on result:
- other: pH not reported
- Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- 1.8
- Temp.:
- 40 °C
- Remarks on result:
- other: pH not reported
- Details on results (HPLC method):
- EQUIPMENT
- Retention times of reference substances used for calibration: 3.970, 4.915, 5.000, 8.050, 9.530, 13.350, 12.750, 15.650, 15.670 (all in minutes)
- Details of fitted regression line (log k' vs. log Koc): A calibration curve was created by using the measured retention times (log k'-values) and the known log K0c-values of the calibration substances for linear regression.
- Average retention data for test substance: 4.730 min. - Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- The adsorption coefficient (Koc) of the test item is: 64 (log Koc = 1.80)
- Executive summary:
The adsorption coefficient (Koc) of the test substance was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Therefore, nine calibration substances were injected into a HPLC-system under the same analytical conditions (column temperature 40 °C) as the test item. A calibration curve was created by using the measured retention times (log k'-values)and the known log Koc-values of the calibration substances for linear regression.The measured log k'-value of the test item was within the calibrated log k'-range. From the resulted calibration curve and its equation the log Koc of the substance had been interpolated. The adsorption coefficient (Koc) of the test item is: 64 (log Koc = 1.80)
Reference
Validity criteria for the measurement of the adsorption/ desorption:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Determinations should be run at least in duplicate. The values of log Koc derived from individual measurements should be within a range of 0.25 log unit. | Number of determinations: 2 The values of log Koc between the individual measurements differed in 0.01 log unit. | Yes |
To correlate the measured HPLC-retention data of a test substance with its adsorption coefficient Koc, a calibration graph of log Koc versus log k’ has to be established. A minimum of six reference points, at least one above and one below the expected value of the test substance should be used. | A calibration graph with nine reference points was established with log Kocs ranging from 1.25 - 4.20. At least one was beneath and above the measured log Koc of the substance. | Yes |
Several mobile phases have been tested and the following two are recommended:
| Methanol / water 55/45 (v/v) (isocratic elution) was used as the mobile phase. | Yes |
Description of key information
The adsorption coefficient (Koc) of the test substance was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Therefore, nine calibration substances were injected into a HPLC-system under the same analytical conditions (column temperature 40 °C) as the test item. A calibration curve was created by using the measured retention times (log k'-values)and the known log Koc-values of the calibration substances for linear regression.The measured log k'-value of the test item was within the calibrated log k'-range. From the resulted calibration curve and its equation the log Koc of the substance had been interpolated. The adsorption coefficient (Koc) of the test item is: 64 (log Koc = 1.80)
Key value for chemical safety assessment
- Koc at 20 °C:
- 64
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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