2-Propenamide, N-(2-hydroxyethyl)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 June 2006 to 20 July 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch number: 050804
Purity: >99%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sampling method: At the start of the definitive test, four samples (20 mL) were taken from the freshly-prepared control and test media. After 48 hours, the contents of the vessels from each group were pooled and four samples (20 mL) were taken for analysis.
- Sample storage conditions before analysis: stored refrigerated

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance (100 mg) was dissolved in dilution medium (1 l) in a volumetric flask. The contents of the flask were shaken and used directly as the test medium.
- Concentration of vehicle in test medium: The range finding test employed nominal test concentrations of 1, 10 and 100 mg/l. Based on the results of this range finding test, the definitive test employed a single concentration, nominally 100 mg/l.
- Evidence of undissolved material: none, substance highly soluble.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Freshwater (Straus)
- Source: Institute National de Recherche Chimique Appliqué (IRChA), France.
- Age at study initiation (mean and range, SD): <24h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test: no feed

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): same
- Type and amount of food: unicellular green alga, Pseudokirchneriella subcapitata
- Feeding frequency: Daily except during the initial three days when a slightly lower ration was given.
- Health during acclimation (any mortality observed): healthy

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

The total hardness and alkalinity of the batch of Elendt M4 medium used were 258 and 36 mg/l as CaCO3, respectively.

Test temperature:

Continuous monitoring of air temperature in the test area = 19.6 to 22.7°C (see protocol deviations).

pH:

7.66 to 8.08

Dissolved oxygen:

93 to 95%

Salinity:

n/a

Nominal and measured concentrations:

nominal 100 mg/l and measured 96.4 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Volume of solution: 100 mL
- Aeration: no supplementary aeration was employed during the exposure period
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 20 mL per organism

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The numbers of mobile, immobile and floating daphnia were counted 24 and 48 hours after the start of the study.

RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 96.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: After 48 hours, one daphnia was observed to be trapped at the surface layer (floating) at 100 mg/L; no immobility had occurred.
- Other biological observations: The measurements of water quality (temperature, pH, dissolved oxygen and total hardness) remained within acceptable limits during the study. The test medium was clear and colourless.

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l.
The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.

Executive summary:

The acute toxicity of HEAA to Daphnia magna was assessed under static exposure conditions. The study was conducted in accordance with OECD Guideline 202, under GLP.

The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.

Description of key information

The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 96.4 mg/L

Additional information

The acute toxicity of HEAA to Daphnia magna was assessed under static exposure conditions. The study was conducted in accordance with OECD Guideline 202, under GLP.

The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.