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EC number: 200-823-7 | CAS number: 74-94-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The acute oral LD50 value of dimethlylamine-borane in rats was reported to be 59.2 mg/kg bw in a publication by Levinskas (1955).
Via the dermal route the acute LD50 value of the test item was determined to be 210.0 respectively 398.0 mg/kg bw in two different in vivo studies similar to OECD 402. In a worst case approach, a dermal LD50 value of 210 mg/kg bw is assumed.
In a review article by Roush (1959) an intraperitoneal LD50 value of dimethylamine-borane in rats of 50.0 mg/kg bw has been reported.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- No guideline available, study was done before guidelines were published.
- GLP compliance:
- no
- Limit test:
- yes
- Specific details on test material used for the study:
- - Melting point: 35.5 - 36.0 °C
- Physical state at room temperature: white crystalline solid - Species:
- rat
- Strain:
- other: CFW
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: adult
- Weight at study initiation: 200-280 g
- Animals fasted for 18 hours
- Route of administration:
- oral: unspecified
- Vehicle:
- not specified
- Details on oral exposure:
- - 24 hours exposure for calculating LD50
- Doses:
- no data
- No. of animals per sex per dose:
- no data
- Control animals:
- not specified
- Details on study design:
- Adult rats were dosed at four different levels.
- Statistics:
- The LD50 values were calculated by the moving average method of Thompson.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 59.2 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no data
- Clinical signs:
- other: no data
- Gross pathology:
- no data
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- In conclusion, the oral LD50 value in rats after treatment with dimethylamine-borane is determined to be 59.2 mg/kg bw.
- Executive summary:
In an acute oral toxicity study, adult CFW rats (male) were given a single oral dose of dimethylamine-borane. An oral LD50 of 59.2 mg/kg bw has been determined.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 59.2 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- to 1979-10-03
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- see box "principles of method if other than guideline"
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: OECD Guideline 404 (Acute Dermal Irritation/ Corrosion)
- Deviations:
- yes
- Remarks:
- see box "principles of method if other than guideline"
- Principles of method if other than guideline:
- Deviations from OECD 404:
- Exposure: 24 h
- Application area: 100 cm^2
- Occlusive
- Tested on the intact and abraded skin
Deviations from OECD 402:
- 4 Animals per dose group (abraded and intact skin 2 each)
- Application area: 100 cm^2
- Occlusive - GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
- Specific details on test material used for the study:
- - Lot: 40-37
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- Twenty-four albino rabbits of the New Zealand White variety weighing apprioxmately 2.5 kg or greater were obtained and acclimated to the laboratory for a minimum of seven (7) days. Rabbits were individually housed in suspended calges with feed and water freely available. The upper back area of all animals was clipped free of fur with an electric clipper.
Animal Care/Diet:
Animal husbandry will be conducted in accordance with the "Guide for Care and Use of Laboratory Animals," DHEW Publication 78-23.
Rabbits will receive Landmark Rabbit Feed on a daily basis. Tap water will be freely available. No contaminants are suspected that would interfere with the interpretation of study results. - Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- Prior to application of the test substance, one half of the rabbits received a series of abrasions, with a sterile needle, through the epidermis. The trunk of each rabbit was wrapped with an impervious material taped in place to form a reservoir over the test skin site. During application, the rabbits were kept in a restrainer device and later were fitted with a collar to prevent ingesting the material and returned to their respective cage.
- Duration of exposure:
- 24 h
- Doses:
- 126, 158, 200, 251, 316 and 398 mg/kg body weight
- No. of animals per sex per dose:
- 2 intact and 2 abraded rabbits per dose
- Control animals:
- no
- Details on study design:
- During application, the rabbits were kept in a restrainer device and later were fitted with a collar and returned to their respective cages. Wrappings were removed after 24 hours and the test material was gently washed from the back.
The rabbits were observed immediately after treatment, at four hours and daily for 14 days for signs of illness and mortality. Dermal reactions were scored daily for erythema and edema. Body weights were recorded at dosing and at 14 days for the survivors. Animals found dead or those euthanized were subjected to a macroscopic examination of the viscera. - Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 210 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 156 - <= 284
- Sex:
- not specified
- Dose descriptor:
- LD0
- Effect level:
- 126 mg/kg bw
- Based on:
- test mat.
- Mortality:
- The LD50 is calculated to be 210 mg/kg body weight with 95% confidence limits of 156-284 mg/kg. For detailed results see Table 1 in "Any other information on results incl. tables
- Gross pathology:
- No macroscopic changes were noted at necropsy which could be attributed to the test material at the highest dose levels. All four rabbits showed uneven pitted appearance in the cortex of the kidney at the lowest level.
- Other findings:
- No significant dermal reactions were observed.
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- In this acute dermal toxicity study, the dermal LD50 value in rabbits after treatment with dimethylamine-borane was 210 mg/kg body weight.
- Executive summary:
In an acute dermal toxicity study similiar to OECD 402, groups of 4 New Zealand White rabbits (2 with intact, 2 with abraded skin) were dermally exposed to dimethylamine-borane for 24 hours at doses of 389, 316, 251, 200, 158 and 126 mg/kg body weight. Animals then were observed for 14 days. The dermal LD50 was determined to be 210 mg/kg bw. There were no treatment related clinical signs and necropsy findings. All surviving rabbits gained weight during the course of the study.
Based on this study, dimethylamine-borane is of slight toxicity. Based on the dermal LD50 of 210 mg/kg bw, the test item does warrant for classification as Acute Tox Cat. 3 according to CLP Criteria.
Reference
Table 1: Mortality
mg/kg | No. Dead/No. Dosed |
398 | 4/4 |
316 | 3/4 |
251 | 2/4 |
200 | 3/4 |
158 | 2/4 |
126 | 0/4 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 210 mg/kg bw
Additional information
In an acute oral toxicity study by Levinskas (1955), groups of rats were given a single dose of Dimethylamine-borane. Based on the mortality, an oral LD50 of 59.2 mg/kg bw was determined. Based on these results, the test item Dimethylamine-borane is of slight toxicity and does warrant for classification as Acute Tox. Cat. 3 according to CLP criteria.
In an acute dermal toxicity study similar to OECD 402, groups of 4 New Zealand White rabbits (2 with intact, 2 with abraded skin) were dermally exposed to Dimethylamine-borane for 24 hours at doses of 389, 316, 251, 200, 158 and 126 mg/kg body weight. Animals then were observed for 14 days. The dermal LD50 was determined to be 210 mg/kg bw. There were no treatment related clinical signs and necropsy findings. All surviving rabbits gained weight during the course of the study.
Based on this study, Dimethylamine-borane is of slight toxicity. Based on the dermal LD50 of 210 mg/kg bw, the test item does warrant for classification as Acute Tox Cat. 3 according to CLP criteria.
In a second acute dermal toxicity study similar to OECD 402, groups of 6 New Zealand White rabbits (3 with intact, 3 with abraded skin) were dermally exposed to Dimethylamine-borane for 24 hours at doses of 389, 282, and 200 mg/kg body weight. Animals then were observed for 14 days. The dermal LD50 was determined to be 398 mg/kg bw. There were no treatment related clinical signs and necropsy findings. All surviving rabbits gained weight during the course of the study.
Based on this study, Dimethylamine-borane is of slight toxicity. Based on the dermal LD50 of 398 mg/kg bw, the test item does warrant for classification as Acute Tox Cat. 3 according to CLP Criteria.
In a review article by Roush (1959) an intraperitoneal LD50 value of Dimethylamine-borane in rats of 50.0 mg/kg bw has been reported. The rats showed progressive depression leading to coma and death was apparently respiratory.
Justification for classification or non-classification
Based on the dermal LD50 value of 210.0 mg/kg bw determined in an in vivo study similar to OECD 402 and the oral LD50 value of 59.2 mg/kg bw published in a review article, the test item dimethylamine-borane does warrant for classification as Acute Tox. Cat. 3 (H301 for acute oral toxicity and H311 for acute dermal toxicity) according to CLP criteria.
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