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EC number: 953-104-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date: December 23, 2020
Experiment completion date: February 19, 2021 - Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of oxybispropanediol and tetraglycerol and triglycerol, esterification products with lactic acid and lauric acid
- Molecular formula:
- not applicable - UVCB substance
- IUPAC Name:
- Reaction mass of oxybispropanediol and tetraglycerol and triglycerol, esterification products with lactic acid and lauric acid
Constituent 1
- Specific details on test material used for the study:
- Test item name: Finestar 2009
CAS Number: 2225876-48-0
Water solubility: soluble in water
Molecular formula: C12 H22 O8
Molecular weight: 294.30
Batch/Lot number: 3253082031
analyzed purity: 100% active
Saponification value: 45.87
Date of manufacture: August 27, 2020
Date of expiry: August 26, 2021
Appearance: Pale yellow viscous liquid
Storage temperature: Room temperature (15 to 30°C)
Storage condition: Keep away from light, moisture, and oxidizing - reducing chemicals
Storage container: Keep in original container
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the main study, six replicates for treatment and control were prepared. 10 mL of test samples from each replicate were drawn and mixed together for each group. The collected samples were centrifuged at 2000 rpm for 10 minutes in order to remove the algal cell. The representative samples were divided into two portions. One portion was sent for test concentration analysis at 0 h and 72 h. the second portion was stored at -20 +/- 5°C.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Solubility test
Test item was found to be soluble in algal medium (OECD medium) at the concentration of 200 mg/mL (Stock A).
Preliminary range-finding study
A preliminary study was conducted with the test concentrations of 0.0 (control), 0.01, 0.1, 1.0, 10.0 and 100 mg Finester 2009/L. two replicates were taken for each concentration of the test item and three replicates for the control group.
The percent inhibition of biomass was 0.67, 0.97, 1.38, 1.98 and 3.40 for the test concentrations of 0.01, 0.1, 1.0, 10.0 and 100 mg/L respectively.
The percent inhibition of growth rate was 0.00, 0.00, 0.00, 0.00 and 0.14 for the test concentrations of 0.01, 0.1, 1.0, 10.0 and 100 mg/L, respectively.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain number: ATCC 22662
Origin and source of culture: American Type Culture collection, Manassas, USA
Date of receipt: March 21, 2018
Date of the last sub-culture: February 08, 2021
Method of cultivation: static condition
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- All test and control cultures were inspected microscopically at 72 hours
Test conditions
- Test temperature:
- 22 - 23°C
- pH:
- 8.00 - 8.08
- Salinity:
- Not applicable as freshwater study
- Nominal and measured concentrations:
- Based on the results of the preliminary range-finding study, the main study was restricted to a limit study. the limit study was conducted with 0.0 (control) and 100 mg/L of test item.
- Details on test conditions:
- Pre-culture
The pre-culture was prepared 2 days prior to the commencement of the study by transferring 0.6 mL from the latest subculture to a final volume of 100 mL into a new culture vessel. The pre-culture was incubated under the same conditions as those required for the test and were used when growing exponentially. The temperature for pre-culture ranged between 22 and 23°C.
Initial cell concentration
Mean cell concentration was derived from 3 samples taken from the pre-culture. The cell concentration of the algal stock was determined as 750,000 cells/mL by manual counting using a haemocytometer and a microscope. Each replicate was inoculated with 0.9 mL of algal culture to obtain the required cell concentration of 5663 cells/mL.
Culturing apparatus
250 mL conical flask
Test culture maintenance
All pre-culture flasks were maintained between 22 and 23°C and within 7033 and 7490 lux (+/- 15% of the mean value) with a universal UV-free white, fluroescent lamp. Illumination and temperature were recorded daily using a light meter and a minimum-maximum thermometer, respectively. The cells in the cultured flasks were maintained in suspension by agitating the test flasks continuously at 100 repm, using an orbital shaker during the test period. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Algal cell count
The cell concentration in the control culture increased by a factor of 97.3 times within the three-day test period.
The coefficient of variation of average specific growth rate during the whole test period (0-3) in replicate control was 0.65%. The mean coefficient of variation for days 0-1, 1-2 and 2-3 in control was 10.27%.
Active ingredient concentration and stability analysis
The stability of the test material in test media was performed during the method validation, and was stable up to 72 hours (>80% of the nominal concentration). The test solution was aalysied for Finester and stability at 0 and 72 h during the limit study and were found to be within the acceptable limit (>80% of the nominal concentration).
Growth inhibition, EC 60 Values, NOEC and LOEC
There was no significant alteration observed in the algal growth (biomass), yield, and the growth rate of the test item exposed in the treatment group as compared to the control group. Finester 2009 did not inhibit the growth of algae up to 100 mg/mL.
Any other information on results incl. tables
Mean Values of Algal Biomass, Yield, specific Growth Rate
Group | Test concentration (mg/L) | Mean number of cells Count/mL at | Specific growth rate (0-3_ | ||||
0 h | 24 h | 48 h | 72 h | Yield | |||
G1 | 0 | 5663 | 25000 | 111250 | 550833 | 545170 | 1.53 |
G2 | 100 | 23333 | 110833 | 549583 | 543920 | 1.53 | |
Validation criteria of study
The coefficient of variation of the average specific growth rates during the test period in the replicate control was 0.65%.
The cell concentration in the control cultures within the test period was increased exponentially by a factor of 97.3%.
The mean coefficient of variation for days 0-1, 1-2 and 2-3 in control cultures was 10.27%.
Thus, the validity criteria were met.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Results of the study indicate that Finestar 2009 exposure caused no effect in the algae growth. Based on concentration-effect values for the biomass inihibition, growth rate inhibition and yield inhibition for cells, it can be concluded that finestar 2009 exposure caused no inhibitory effect on the growth. EC50, NOEC and LOEC values were greater than 100.0 mg/L.
- Executive summary:
This study was performed to assess effects on the growth of the alga Pseudokitchneriella subcapitata, caused by exposure under static conditions, of Finestar 2009.
Exponentially growing cultures of alga were exposed to the nominal test concentrations of 0.0 (Control) and 100 mg/L. Algal cultures were assessed for their growth by visual cell count at 24, 48 and 72 hours.
The stability of finester 2009 in test media was performed during the method validation and Finester 2009 in the test media was stable up to 72 hours (>80% of nominal concentration). The test solution was analysed for chlorantraniliprole and novaluron concentration and stability at 0 and 72 hrs during the limit study and were found to be within the acceptable limnit (>80% of nominal concentration).
there was no significant alteration observed in the algal growth (biomass), yield, and growth rate of the group exposed to finester 2009 when compared to the control group. finester 2009 does not inhibit the growth of alga (P. subcapitata) up to 100 mg/L. The EC50, NOEC and LOEC values were greater than 100 mg/L.
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