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EC number: 410-690-9 | CAS number: 103055-07-8 CGA 184699
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Mar 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Deviations:
- yes
- Remarks:
- See 'Deviations' in 'Any other information on materials and methods incl. tables'
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - For making the test solution, 22.36, 6.98, 2.88, 0.93 and 0.28 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 mL with water and aerated for 3 hours.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 20/03/90
- Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- - Norminal concentration: 0 (Control), 1.4, 4.65, 14.40, 39.40 and 111.80 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL BOD flasks with gas inlet
- Sludge concentration: 1.7 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated drinking water
- Test medium: same as OECD TG 209 suggested
EFFECT PARAMETERS MEASURED
Oxygen consumption per hour in mg/liter was measured. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenole
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- An overview of the results is provided in Table 1 in 'Any other information on results incl. tables'.
After 3 hours incubation, 7%, -5%, 2%, 2% and 8% inhibition on the oxygen consumption were observed in 1.4, 4.65, 14.40, 39.40 and 111.80 mg/L groups, respectively. - Results with reference substance (positive control):
- An overview of the results is provided in Table 1 in 'Any other information on results incl. tables'.
After 3 hours incubation, 15%, 35% and 74% of inhibition on oxygen consumption were observed in 3.2, 10 and 32 mg/L 3,5-Dichlorophenole treated groups, respectively. The EC50 value was determined to be 14.2 mg/L. - Reported statistics and error estimates:
- The inhibitions were calculated on the basis of the measured time depend oxygen consumption of a Blank and Test solution.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an toxicity study performed in accordance with OECD TG 209, the 3-hour EC50 value was determined to be > 100 mg/L for inhibition of oxygen consumption of the microorganisms in activated sludge.
- Executive summary:
A test for inhibitory concentration on aerobic bacteria of the test substance was performed according to OECD TG 209 and GLP principles. Activated sludge collected from sewage treatment plants was placed in test bottles to which the test substance was added (no stock could be made due to low solubility). The final concentrations of the test substance were 0 (blank control),1.4, 6.0, 4.65, 14.4, 34.9 and 111.8 mg/L. 3,5-Dichlorophenole was used as positive control and tested at concentrations of 3.2, 10 and 32 mg/L under the same experimental condition. The filled volume in the test bottle was adjusted to 200 mL with water and aerated for 3 hours. Oxygen consumption per hour was recorded over a 3-hour period at 20 ± 2 °C and the inhibitions calculated on the basis of the measured time dependent oxygen consumption of a blank compared with the test solution. Calculations were based on nominal test concentrations.
After 3 hours incubation, 15%, 35% and 74% of inhibition on oxygen consumption were observed in 3.2, 10 and 32 mg/L 3,5-Dichlorophenole treated groups, respectively. For the test substance treated groups, 8% (in 111.8 mg/L group) or less inhibition was observed. Therefore, the test substance EC20, EC50 and EC80 were all determined to be > 100 mg/L.
Reference
Table 1. Effects on oxygen consumption rate of activated sludge
Nominal Concentration (mg test substance/L) |
Concentration (mg/L) |
Consumption rate (mg/L/h) |
Inhibition (%) |
Blank 1 |
0 |
57.32 |
4 |
Blank 2 |
0 |
62.57 |
-4 |
Reference 1 |
32.0 |
15.73 |
74 |
Reference 2 |
10.0 |
38.97 |
35 |
Reference 3 |
3.2 |
50.94 |
15 |
Test conc 1 |
111.8 |
55.40 |
8 |
Test conc 2 |
34.9 |
58.50 |
2 |
Test conc 3 |
14.4 |
58.70 |
2 |
Test conc 4 |
14.64 |
62.80 |
-5 |
Test conc 5 |
1.4 |
55.91 |
7 |
Description of key information
3-h EC50 > 100 mg/L, Activated sludge, inhibition of total respiration, OECD TG 209, Bader 1990
Key value for chemical safety assessment
Additional information
One study is available for this endpoint, which was selected as key study. In this study performed according to OECD TG 209 and in compliance with GLP, activated sludge collected from sewage treatment plants was exposed to the test substance (no stock could be made due to low solubility) at concentrations of 0 (control),1.4, 6.0, 4.65, 14.4, 34.9 and 111.8 mg/L for 3 hours at 20 ± 2 °C. 3,5-Dichlorophenole was used as positive control and tested at concentrations of 3.2, 10 and 32 mg/L under the same experimental condition. The filled volume in the test bottle was adjusted to 200 mL with water and aerated for 3 hours. Oxygen consumption per hour was recorded over a 3-hour period. After 3 hours incubation, 15%, 35% and 74% of inhibition on oxygen consumption were observed in 3.2, 10 and 32 mg/L 3,5-Dichlorophenole treated groups, respectively. For the test substance treated groups, 8% (in 111.8 mg/L group) or less inhibition was observed. Therefore, the test substance EC20, EC50 and EC80 were all determined to be > 100 mg/L.
Metabolites - available information
In addition to the study with the test substance, two OECD TG 209 followed studies are available with degradation product M6 and M7. As the dossier has been prepared to address the test substance itself, these studies are not summarized as endpoint study records but are briefly discussed here. Both 3-h inhibition of total respiration studies (one with M6 and one with M7), performed in compliance with GLP conditions, showed EC50 > 100 mg/L (Weinstock 1993 and Bader 1988).
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