Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 612-154-1 | CAS number: 6147-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3-6 April 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The vessel temperature in the range-finding test was outwith the 21 – 24 °C (±2 °C) range.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- 66% purity
Aqueous solution - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 278/4
- Source: stock laboratory cultures
- Method of cultivation: autoclaved freshwater at 120 °C for 15 minutes with nutrient medium (3N-BBM+V) 7 days before test commencement. They were then incubated at approximately 21 2 °C under constant illumination to be used as the inoculum source for subsequent toxicity tests. The initial inoculum was checked microscopically using a haemocytometer.
ACCLIMATION
- Acclimation period: 7 days
- Culturing media and conditions same as test
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Not specified
- Test temperature:
- 21 to 24 °C controlled at 2 °C
- pH:
- 7.5 ±0.1. Not increased during the test by more than 1.5 relative to the pH of the growth medium
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 1, 3.2, 10, 32, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass conical flasks
- Material, size, headspace, fill volume: borosilicate, 100ml
- Aeration: flasks were loosely covered with aluminium foil caps and mounted on an orbital shaker
- Initial cells density: approximately 5 x 10^3 – 10^4 cells per millilitre
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, US EPA culture medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The purified water used in this test was treated by reverse osmosis followed by electro-deionisation. This process produces water of an equivalent grade to deionised water. The purified water was held in two 75 litre header tanks until use.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: pH of the culture medium was adjusted if required, by adding 1M HCl, or NaOH to give a pH of 7.5 ±0.1.
- Light intensity and quality: Between 4440 to 8880 lx. The illumination consisted of 40 W cool white LED light which was mounted at a distance of approximately 40 cm directly above the test area.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/l with one replicate per concentration; plus, four controls
- Test concentrations: The definitive test employed five concentrations (1, 3.2, 10, 32, 100 mg/l) with three replicates per concentration; plus, six controls.
- Results used to determine the conditions for the definitive study: Calculated 72h EC(r)50 in the range-finding test was 5.33 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 5.11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- ca. 9.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 3.43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 1.16 mg/L - Reported statistics and error estimates:
- Results for both range-finding and definitive tests were calculated by linear regression within the CETIS suite of statistical analysis.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L.
- Executive summary:
The aquatic phase toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater unicellular algae Pseudokirchneriella subcapitata was determined during a GLPcompliant study performed according to the OECD Testing Guideline 201.
A range-finding test was conducted over 72h to determine the approximate concentrations at which growth rate effects were observed.
The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/L with one replicate per concentration; plus, four controls.
As a result of this range-finding test, concentrations of 1, 3.2, 10, 32, 100 mg/L were used during the definitive test.
The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L.
Reference
Description of key information
The aquatic phase toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater unicellular algae Pseudokirchneriella subcapitata was determined during a GLPcompliant study performed according to the OECD Testing Guideline 201.
A range-finding test was conducted over 72h to determine the approximate concentrations at which growth rate effects were observed.
The range-finding test employed four concentrations 1000, 100, 10 and 1 mg/L with one replicate per concentration; plus, four controls.
As a result of this range-finding test, concentrations of 1, 3.2, 10, 32, 100 mg/L were used during the definitive test.
The 72-hour EC50 was ca. 5.11 mg/L and the 72-hour NOEC was ca. 1 mg/L.
The EC50 for the substance was 5.11 mg/L and the substance is not readily biodegradable. Therefore, the substance is classifield as aquatic toxicity category 2.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 5.11 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.