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EC number: 413-750-2 | CAS number: 171090-93-0 ANOX 1315; ANOX BF; DURAD AX 38
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 July 1993 - 02 August 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- After 30 minutes of aeration at 22˚C, the first sample was taken for oxygen measurement over a period of up to 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration.
Sampling was started with the control 1. Thereafter, the measurements were continued with the positive controls and the test article samples. Finally, the oxygen consumption of control 12 was determined, - Details on test solutions:
- ANOX BF (stock solution 1):
0.5 g ANOX BF was suspended with 0.5 g Tween 80 and filled to a volume of 1000 ml with bidistilled water. The final tween 80 concentration was 0.01%.
Tween 80 solution (stock solution II):
A Tween 80 solution contained 1 g Tween 80/l was prepared. The final Tween 80 concentration was 0.01%.
3,5-dichlorophenol (stock solution III):
3,5-dichlorophenol was used as a positive standard. Therefore 0.5 g of standard were dissolved according to the OECD Guideline No. 209 in 10 ml 1N NaOH, diluted to about 30ml with bidistilled water. Excess of NaOH was neutralized with 1N H2SO4 to the point of incipient precipitation. Therefore, the mixture was made up to 1 litre. The final pH was 7.8 - Test organisms (species):
- anaerobic bacteria from a domestic water treatment plant
- Details on inoculum:
- The test was run with aerobic activated sludge from a domestic waste-water treatment plant (ARA, Fullinsdorf, Ergolz II/Switzerland).
The sludge was centrifuged and the supernatant liquid phase decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated a further two times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (ml) to its dry weight (g) determined. Based on this ratio, a volume of washed sludge suspension corresponding to 4.0g dry material were made up with Soerensen buffer (pH =) to 1 litre. To that mixture, 50ml OECD recommended synthetic sewage feed were added. Thereafter, the sludge was aerated until use. The pH measured was 7.9.
Synthetic sewage feed (filled up to a final volume of 1 litre with bidistilled water):
16g peptone
11g meat extract
3g urea
0.7NaCl
0.4g CaCl2 . 2H2O
0.2g MgSO4 . 7H2O
2.8g K2HPO4 - Test type:
- other: aerobic
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 0.5 h
- Hardness:
- Not specified
- Test temperature:
- Not specified
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 0.72 mg/O2/l min (mean)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The respiration rate of the sludge was not inhibited. The inhibition rates ranged from -23% to 2.1% for the concentration range 3.2 mg/l to 100 mg/l. The inhibition at 10mg/l could not be calculated due to the rapid oxygen consumption.
Based on these results the EC50 can be defined as >100mg/l - Results with reference substance (positive control):
- At the two lowest concentrations of the standard of 1 mg/l and 3.2 mg/l, the respiration rate was inhibited by 2.1 and 4.9%, respectively. At 10 mg/l, the inhibition was 30.1%. At the two highest concentration of 32 and 50 mg/l, the inhibition rate was 86.0%.
y=56.392 x -11.293 (R=0.948)
(x represents the logarithm of dose of 3,5-dichlorophenol and Y the % inhibition).
According to this equation the EC50 was 12.2 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibitory effect of ANOX BF on aerobic waste-water bacteria was investigated in a respiration test.
The test article showed no inhibition on the respiration rate up to a test concentration of 100mg/l. The EC50 can be defined as > 100 mg/l.
The EC50 of the reference compound 3,5-dichlorophenol tested under the same conditions was: 12.2 mg/l which in the guideline accepted range. - Executive summary:
The test article showed no inhibition on the respiration rate up to a test concentration of 100mg/l. The EC50 can be defined as > 100 mg/l.
Reference
Description of key information
The inhibitory effect of ANOX BF on aerobic waste-water bacteria was investigated in a respiration test.
The test article showed no inhibition on the respiration rate up to a test concentration of 100mg/l. The EC50 can be defined as > 100 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
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