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EC number: 838-538-0 | CAS number: 52139-31-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: test meets generally accepted principles but with insufficient documentation, test performed prior to implementation of standard methods
Data source
Reference
- Reference Type:
- publication
- Title:
- OXIDATION OF CERTAIN AMINO ACIDS BY “RESTING” BACILLUS PROTEUS
- Author:
- FREDERICK BERNHEIM, MARY L. C. BERNHEIM, AND M. DOROTHY WEBSTER
- Year:
- 1 935
- Bibliographic source:
- Jour. Biol. Chem., 110, 165 (1935).
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test:
Bacillus proteus Type X-2 was grown for 18 hours on beef extract agar slants at 37° and pH 7.4. Subsequently, 1 cc. of the suspension of bacteria was used in each Warburg vessel and the volume made up to 2 cc. with buffer and a solution of the amino acid to be tested.
- Short description of test conditions: The bacteria were then scraped off the slants, suspended in saline, and centrifuged. This washing was
repeated three times and then the bacteria were suspended in 0.05 M phosphate buffer of pH 7.8 so that there were approximately 7 billion per cc. The optimum pH for the oxidation of the amino acids is between 7.6 and 8.3.
- Parameters analysed / observed: The rate and amount of the oxygen uptake was then measured. - GLP compliance:
- no
Test material
- Reference substance name:
- Glycine
- EC Number:
- 200-272-2
- EC Name:
- Glycine
- Cas Number:
- 56-40-6
- Molecular formula:
- C2H5NO2
- IUPAC Name:
- aminoacetic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 6 h
Initial test substance concentration
- Initial conc.:
- 0.25 other: mg
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: Warburg vessels
Reference substance
- Reference substance:
- not specified
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 104
- Sampling time:
- 110 min
- Remarks on result:
- other: Observed O2 uptake
- Remarks:
- sampling time gives the oxidation rate based on the time necessary to reach one-half the final uptake
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In the present study of Bernheim et al. 'resting' Bacillus proteus were incubated with different amino acids, i.a. with 0.25 mg/cm³ glycine. The determined oxidation rate was 110 min and the oxygen uptake was 104 cm³. Furthermore, it was reported that glycine was completely metabolised in contrast to the other amino acids investigated. Thus, glycine is considered to be readily biodegradable under the conditions of the test.
- Executive summary:
The biodegradation of glycine was determined in Bacillus proteus. The method of determination is considered sufficient, the study was conducted prior to implementation of standard guidelines. Bacillus proteus Type X-2 was grown for 18 hours on beef extract agar slants at 37° and pH 7.4. Subsequently, a suspension of bacteria was used in each Warburg vessel and the volume made up to 2 cc. with buffer and a solution of the amino acid to be tested. The oxidation rate was measured after 6h. The authors were able to show that glycine in contrast to all other proteinogenic amino acids was fully metabolised by resting Bacillus proteus. Thus, glycine is considered readily biodegradable under the conditions of the test.
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