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EC number: 250-784-5 | CAS number: 31736-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 28 - Nov 10, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure and at the renewal (0 and 24 h), samples of the freshly prepared test item concentrations were taken and analyzed.
At renewal and at the end of the exposure (24 and 48 h), sampled of the 24 h old media were taken directly fron the test vessels and analyzed. - Vehicle:
- yes
- Remarks:
- dilution water
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden und Lufthygiene (WaBoLu), Berlin, Germany
- Age: < 24 h
FEEDING DURING TEST
- Food type: 5 x per week ad libitum with a mix of unicellular green algae - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 20.1 to 20.5 °C
- pH:
- 7.8 to 8.0
- Dissolved oxygen:
- 8.5 to 8.8 mg/L
- Conductivity:
- 330 to 510 lux
- Nominal and measured concentrations:
- The following dilutions of a filtrate of 100 mg/L were tested: 1:100, 1:220, 1:484, 1:1065 and 1:2343. and a control, corresponding to following time weighted average concentrations of the test item:
60.6, 25.4, 10.6, 4.88 and 3.15 µg test item/L, and a control. - Details on test conditions:
- Test vessels:
Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.
Number of daphnids and replicates: This study encompassed 6 treatment groups (5 dose rates of the test item and a control) each containing 20 individuals.
Application: The test item is not well soluble in test medium. To avoid physical effects of undissolved test item on the daphnids, no concentrations above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 88.2 and 100.7 mg test item in 882 and 1007 mL test water. The stock suspension was stirred for 6 hours at room temperature in the dark to dissolve as much test item as possible. Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtration (0.45 µm cellulose acetate filter). The solution with dissolved test item was used to prepare the dilutions of 1:100, 1:220, 1:484, 1:1065 and 1:2343.
The test media were prepared just before introduction of the daphnids (= start of the test) and test medium renewal on Day 1. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.016 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 10.6 µg test item/L. At the concentrations of 25.4 and 60.6 µg test item/L all animals were immobile
- Results with reference substance (positive control):
- EC50 (48 h) = 0.986 mg/L
valid range: 0.6 - 2.1 mg/L, acc. to OECD 202 - Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item to Daphnia magna was assessed in a semi-static concentration-response test.
48-hour NOEC: 10.6 µg test item/L.
48-hour LOEC: 25.4 µg test item/L
48-hour EC50: 16.0 µg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to time weighted average concentrations, since the test item concentrations were not within ± 20 % of the measured initial concentrations during the test.
Reference
Influence of the test material on the Mobility of Daphnia magna and Sublethal Effects
Time Weighted Average Concentration [µg test item/L] |
No. of Daphnia tested |
No of immobilised Daphnia after |
% of immobilised Daphnia after |
Observations after |
|||
24 h |
48 h |
24 h |
48 h |
24 h |
48 h |
||
Control |
5 |
0 |
0 |
0 | 0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
3.15 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
4.88 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
10.6 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
5 |
0 |
0 |
0 |
0 |
|||
25.4 |
5 |
3 |
5 |
65 |
100 |
0 |
0 |
5 |
4 |
5 |
0 |
0 |
|||
5 |
3 |
5 |
0 |
0 |
|||
5 |
3 |
5 |
0 |
0 |
|||
60.6 |
5 |
5 |
5 |
100 | 100 |
0 |
0 |
5 |
5 |
5 |
0 |
0 |
|||
5 |
5 |
5 |
0 |
0 |
|||
5 |
5 |
5 |
0 |
0 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 16 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.