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EC number: 457-280-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22.09.2022 to 23.11.2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted 13th April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L including control group.
- Sampling method: Analysis of the test substance was performed in each replicate at the beginning(0 h) and 48 h after exposure - Vehicle:
- no
- Details on test solutions:
- The required amount of the test substance 0.1002 g was weighted using a balance(Ohaus, U.S.A.)
into 1 L volumetric flask and the test water(M4 medium) was added until the 1 L for preparing of
100.00 mg/L. This solution was sealed and mixed using a magnetic stirrer(Misung Scientific. Co.,
Ltd., Korea) for 24 h and was filtered through a 0.45 µm PVDF membrane filter(Cuota Lab, Korea).
Two same 100.00 mg/L was prepared according to the above preparation method. 62.5, 125.0, 250.0
and 500.0 mL of the stock solution was added into each 1 L volumetric flask to prepare test
solution(6.25, 12.50, 25.00 and 50.00 mg/L) and final volume was adjusted to 1 L using the test water.
The remaining stock solution was used as the test solution(100.00 mg/L). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- < 24 h old, third generation neonates.
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Elendt M4 Medium
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Hardness(Test water) : 160 mg CaCO3/L
Alkalinity(Test water) : 30 mg CaCO3/L - Test temperature:
- 20.3°C (20.1~20.5°C)
- pH:
- 7.62(7.11~7.90)
- Dissolved oxygen:
- 8.31 mg/L(7.83~8.51 mg/L)
- Nominal and measured concentrations:
- Nominal: 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L
Geometric mean measured: 5.885, 11.527, 23.097, 45.329 and 94.094
mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: separation test dish(Φ80×45 H (mm)) in the water bath(20±2 ˚C)
- Volume of solution: 100 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding: None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 Medium
- Intervals of water quality measurement: 0 and 48 h
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 24 h continuous darkness
- Light intensity: not mentioned
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Observations every 24 h
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0.10, 1.00, 10.00, 100.00 mg/L and a control group(0 mg/L)
- Results used to determine the conditions for the definitive study: 5 Daphnia magna per group were used at treatment group and control group during an exposure period of 48 h under the semi static condition. results not mentioned.
On the basis of the result of the range finding test, the treatment group of the definitive test was composed
to be 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L including the control group. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24.77 mg/L
- 95% CI:
- 20.348 - < 30.309
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- % Immobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- % Immobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- % Immobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 36.234 mg/L
- 95% CI:
- 28.912 - < 45.228
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- % Immobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study the result of EC50 24.8 mg/L means that the test substance is classified as Aquatic Chronic 3 H412.
- Executive summary:
This study evaluated acute immobilisation caused by the test substance, in the Daphnia magna. In accordance with the guidelines for range finding test, the treatment group received 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L of the test substance. The treatment and a control group remained under a static condition for 48 h, and then the median effective concentration(EC50) was calculated. The solubility test, stability test, range finding test and definitive test were conducted under thedarkness conditions based on the storage conditions(darkness) of the test substance.
Solubility test was conducted with a test solution of 100.00 mg/L. The method that stirring for 24 h resulted in the highest solubility in the test water. So, this method applied to this study. The stability test was conducted with a test solution of 100.00 mg/L. The result was stable to
102.4 %(0~48 h) in the 100.00 mg/L test solution. This result was 80~120 % of the initial measured concentration. So, this study was conducted under the static condition. On the basis of the result of the range finding test, this test was conducted of the definitive test, the
treatment group was composed to 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L including control group. As a result of the definitive test, the geometric mean measured concentration at the treatment group(6.25, 12.50, 25.00, 50.00 and 100.00 mg/L) was 5.885, 11.527, 23.097, 45.329 and 94.094 mg/L. These results were 94.2, 92.2, 92.4, 90.7 and 94.1 % of the nominal concentration. This result was 80~120 % of the nominal concentration. Therefore, the test results were determined based on the nominal concentration.
1) The result is as follows.
Observation Time (h)
EC50a (mg/L)
95 % Confidence limits (mg/L)
The highest concentration causing no immobility or mortality (mg/L) The lowest concentration producing 100 % immobility or mortality (mg/L)
24 36.234 28.912~45.228 12.50 - b 48 24.777 20.348~30.309 6.25 100.00
a: Median effective concentration, based on nominal concentration
b: Not applicable
2) Result measurement of test solution(minimum~maximum)
Average, range of temperature : 20.3 C (20.1~20.5 C)
Average, range of Dissolved : 8.31 mg/L(7.83~8.51 mg/L)
Oxygen(D.O.) 91.5 %(85.9~94.1 %)
Average, range of pH : 7.62(7.11~7.90)Hardness(Test water) : 160 mg CaCO3/L
Alkalinity(Test water) : 30 mg CaCO3/L
Reference
Description of key information
Under the conditions of this study the result of EC50 24.8 mg/L means that the test substance is classified as Aquatic Chronic 3 H412.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 24.777 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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