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EC number: 850-698-3 | CAS number: 2387913-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental Start Date: 03 April 2020, Experimental Completion Date: 02 May 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Annex VIII Data Requirement
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442D (In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method)
- Version / remarks:
- June 2018
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- activation of keratinocytes
- Justification for non-LLNA method:
- In Chemico / in vitro tests are required to be performed prior to the performance of in vivo studies (Section 8.3.1 of Annex VII of the REACH Regulation).
Test material
- Reference substance name:
- N,N,N-tri-C8-C10-alkyl-N-methyl-1-aminium sulfate
- EC Number:
- 850-698-3
- Cas Number:
- 2387913-24-6
- Molecular formula:
- not applicable
- IUPAC Name:
- N,N,N-tri-C8-C10-alkyl-N-methyl-1-aminium sulfate
- Test material form:
- other: solified melt
- Details on test material:
- - Name of test material ((as cited in study report):
Product name: V10069,
Systemic name: Quaternary ammonium compounds, tri-C8-10-alkylmethyl-methylsulfate
Constituent 1
Results and discussion
- Positive control results:
- The luciferase activity induction obtained with the positive control, Ethylene dimethacrylate glycol, was statistically significant above the threshold of 1.5-fold in at least one concentration.
The EC1.5 of the positive control (37 µM) was within two standard deviations of the historical mean in the second experiment. The EC1.5 of the positive control (125 µM) was not within two standard deviations of the historical mean (-4.3 – 123 µM) in the first experiment. The purpose of the positive control is as a reference for the test system, where a positive response is required to check if the test system is sensitive enough. A clear dose-response with increasing luciferase activity induction at increasing concentrations was observed. Furthermore, the value was only slightly higher, all other acceptability criteria were met and an independent repeat experiment was performed to determine the effect of the assay in the KeratinoSens assay.
A dose response was observed and the induction at 250 µM was higher than 2-fold
(2.16-fold and 3.74-fold in experiment 1 and 2, respectively).
In vitro / in chemico
Resultsopen allclose all
- Key result
- Run / experiment:
- other: 1
- Parameter:
- other: EC1.5
- Positive controls validity:
- valid
- Remarks on result:
- no indication of skin sensitisation
- Remarks:
- The Imax was 0.81 and therefore no EC1.5 could be calculated.
- Key result
- Run / experiment:
- other: 2
- Parameter:
- other: EC1.5
- Positive controls validity:
- valid
- Remarks on result:
- no indication of skin sensitisation
- Remarks:
- The Imax was 1.16 and therefore no EC1.5 could be calculated.
Any other information on results incl. tables
Two independent experiments were performed.The cells were in these experiments incubated withthe test itemin a concentration range0.098– 200 µg/mL (2-fold dilution series, first experiment) andin test concentrations of 0.005– 10 µg/mL (2-fold dilution series, second experiment) for 48 hours± 1 h. The activation of the ARE-dependent pathway was assessed by measuring the luminescence induction compared to the vehicle control. In addition, the viability was assessed with an MTT assay.
Experiment 1
· Precipitation
was observed at the start of the incubation period at test
concentrations of 100 µg/mL and upwards and at the end of the incubation
period at test concentrations
200 µg/mL in the 96-well plates.
· The test item showed toxicity. The calculated IC30was 0.38 µg/mL and the calculated IC50was 0.52 µg/mL.
· No luminescence activity induction compared to the vehicle control was observed at any of the test concentrations after treatment with the test item. The Imaxwas 0.81 and therefore no EC1.5could be calculated.
· The positive control Ethylene dimethacrylate glycol caused a dose related induction of the luciferase activity. The Imaxwas 2.16 and the EC1.5125 µM.
Experiment 2
· No precipitation was observed at the start and end of the incubation period in the 96-well plates.
· The test item showed toxicity. The calculated IC30was 0.37 µg/mL and the calculated IC50was 0.48 µg/mL.
· No luminescence activity induction compared to the vehicle control was observed at any of the test concentrations after treatment with the test item. The Imaxwas 1.16 and therefore no EC1.5could be calculated.
· The positive control Ethylene dimethacrylate glycol caused a dose related induction of the luciferase activity. The Imaxwas 3.74 and the EC1.537 µM.
Both tests passed the acceptance criteria:
· The luciferase activity induction obtained with the positive control,Ethylene dimethacrylate glycol, was statistically significant above the threshold of 1.5-fold in at least one concentration.
· The EC1.5of the positive control (37 µM) was within two standard deviations of the historical mean in the second experiment. The EC1.5of the positive control (125 µM) was not within two standard deviations of the historical mean (-4.3 – 123 µM) in the first experiment. The purpose of the positive control is as a reference for the test system, where a positive response is required to check if the test system is sensitive enough. A clear dose-response with increasing luciferase activity induction at increasing concentrations was observed. Furthermore, the value was only slightly higher, all other acceptability criteria were met and an independent repeat experiment was performed to determine the effect of the assay in the KeratinoSensÔassay.
A dose
response was observed and the induction at 250 µM was higher than 2-fold
(2.16-fold and 3.74-fold in experiment 1 and 2, respectively).
· Finally, the average coefficient of variation of the luminescence reading for the vehicle (negative) control DMSO was below 20% (10% in experiment 1 and 2).
Overall it is concluded that the test conditions were adequate and that the test system functioned properly.
Overview Luminescence Induction and Cell Viability of test item in Experiment 1 and 2
Concentration (µg/mL) |
0.098 |
0.20 |
0.39 |
0.78 |
1.6 |
3.1 |
6.3 |
12.5 |
25 |
50 |
100 |
200 |
Exp 1 luminescence |
0.77 |
0.73 |
0.81 |
0.61 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
Exp 1 viability (%) |
114.8 |
115.9 |
68.2 |
12.0 |
-0.1 |
-0.1 |
0.0 |
0.0 |
0.0 |
0.1 |
0.2 |
0.6 |
Concentration (µg/mL) |
0.005 |
0.01 |
0.02 |
0.04 |
0.08 |
0.16 |
0.31 |
0.63 |
1.3 |
2.5 |
5 |
10 |
Exp 2 luminescence |
0.87 |
1.11 |
1.16 |
1.11 |
1.08 |
1.14 |
0.95 |
1.01 |
0.00 |
0.00 |
0.00 |
0.00 |
Exp 2 viability (%) |
89.8 |
98.1 |
100.0 |
101.8 |
110.2 |
103.6 |
79.3 |
24.8 |
-0.8 |
-0.7 |
-0.8 |
-0.7 |
Overview Luminescence Induction and Cell Viability Positive Control EDMG
in Experiment 1 and 2
Concentration (µM) |
7.8 |
16 |
31 |
63 |
125 |
250 |
Exp 1 luminescence |
0.84 |
0.95 |
1.00 |
1.20 |
1.50*** |
2.16*** |
Exp 1 viability (%) |
116.2 |
107.9 |
108.3 |
102.4 |
92.0 |
86.9 |
Exp 2 luminescence |
0.91 |
1.20 |
1.46 |
1.69*** |
2.47*** |
3.74*** |
Exp 2 viability (%) |
92.1 |
92.0 |
81.6 |
74.4 |
77.5 |
67.5 |
***p<0.001 Student’s t test
Overview EC1.5, Imax, IC30and IC50Values
|
EC1.5(µg/mL) |
Imax |
IC30(µg/mL) |
IC50(µg/mL) |
Test item Experiment 1 |
NA |
0.81 |
0.38 |
0.52 |
Test item Experiment 2 |
NA |
1.16 |
0.37 |
0.48 |
|
EC1.5(µM) |
Imax |
IC30(µM) |
IC50(µM) |
Pos Control Experiment 1 |
125 |
2.16 |
NA |
NA |
Pos Control Experiment 2 |
37 |
3.74 |
219 |
NA |
NA = Not applicable
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- It was concluded that the test item gave a negative response in the ARE-Nrf2 Luciferase Test (KeratinoSens™), supporting the prediction that the test item is not a skin sensitizer.
- Executive summary:
The test item showed toxicity (IC30values of 0.38µg/mL and 0.37µg/mL and IC50values of 0.52µg/mL and 0.48 µg/mL in experiment 1 and 2, respectively). No biologically relevant induction of the luciferase activity (no EC1.5value) was measured at any of the test concentrations in both experiments. The maximum luciferase activity induction (Imax) was 0.81-fold and 1.16-fold in experiment 1 and 2 respectively. The test itemis classified as negative in the KeratinoSensTMassaysince negative results (<1.5-fold induction) were observed at test concentrations up to 200µg/mL.
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