2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol hydrochloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to section 13 for the read-across justification.

Reason / purpose for cross-reference:

read-across source

Key result

Parameter:

% degradation (CO2 evolution)

Value:

11.89

Sampling time:

28 d

Description of key information

In a study according to OECD TG 301B under GLP, the read-across substance is found to be inherently, primarily biodegradable (reference 5.2.1 -1).

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable, not fulfilling specific criteria

Type of water:

freshwater

Additional information

No study data with the test item is available for ready biodegradability. Therefore, a read-across to the read-across substance with a very similar chemical structure and comparable physico-chemical parameters is used to evaluate the biodegradation potential of the test item.

 

The read-across substance was investigated for its ready biodegradability in a study according to OECD TG 301B under GLP at the concentration of 33 mg/L. The test was performed over a period of 28 days with biodegradation that was followed by CO2-evolution. As a reference item, sodium benzoate was tested simultaneously under the same conditions as the read-across substance, and functioned as a procedure control. The percentage biodegradation of the read-across substance reached a mean of 11.98 % after 28 days. The reference item Sodium benzoate was sufficiently degraded to 61.74 % already after 8 days, and to 76.22 % after 28 days of incubation, based on its ThCO2, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both the read-across substance and the reference item, a mean of 27.25 % biodegradation was noted within 8 days and 35.52 % biodegradation after 28 days of incubation. Since all acceptability criteria prescribed by the protocol were met, this study was considered valid. In conclusion, the read-across substance was considered to be inherently, primarily biodegradable, as a 10-d-window could not be determined during the 28-day test.