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EC number: 701-329-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Remarks:
- review article with limited information
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- THE RESULTS OF MICROBIAL MUTATION TEST FOR FORTY-THREE INDUSTRIAL CHEMICALS
- Author:
- Shimizu, H. et al.
- Year:
- 1 985
- Bibliographic source:
- Jpn. J. Ind. Health. 27:400 - 419
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted July 1997
- Deviations:
- yes
- Remarks:
- Positive control substance for TA 1535 (without S9-mix) differs from the guideline. S9 was prepared from rats treated with polychlorinated biphenyl (KC 500).
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Adipic acid
- EC Number:
- 204-673-3
- EC Name:
- Adipic acid
- Cas Number:
- 124-04-9
- Molecular formula:
- C6H10O4
- IUPAC Name:
- adipic acid
Constituent 1
Method
- Target gene:
- his operon and trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the liver of male rats treated with polychlorinated biphenyl (KC 500) at a dose of 500 mg/kg bw
- Test concentrations with justification for top dose:
- all strains: 1, 5, 10, 50, 100, 500, 1000 and 5000 µg/plate with and without metabolic activation
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- details are given below (see additional information on materials and methods)
- Details on test system and experimental conditions:
- DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: The test was performed in duplicate.
DETERMINATION OF CYTOTOXICITY
The background bacterial lawn was checked by a dissection. - Statistics:
- For the test item, mean values of the duplicates are shown whereas mean values and standard deviations are given for the positive control substances and vehicle controls.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1. Test results
With or without S9-Mix |
Test item concentration (µg/plate) |
Mean number of revertant colonies/plate ± standard deviation |
|||||
Base-pair substitution type |
Frameshift type |
||||||
TA100 |
TA1535 |
WP2 uvrA |
TA98 |
TA1537 |
TA1538 |
||
- |
vehicle control (DMSO) |
150 ± 16.8 |
30 ± 5.6 |
30 ± 9.7 |
32 ± 7.7 |
18 ± 8.6 |
22 ± 7.3 |
- |
1 |
152 |
40 |
58 |
27 |
11 |
15 |
|
5 |
148 |
43 |
46 |
30 |
9 |
18 |
- |
10 |
151 |
32 |
46 |
26 |
6 |
15 |
- |
50 |
155 |
36 |
50 |
23 |
9 |
15 |
- |
100 |
179 |
42 |
40 |
29 |
6 |
20 |
- |
500 |
154 |
35 |
44 |
31 |
10 |
16 |
- |
1000 |
150 |
37 |
43 |
27 |
7 |
18 |
- |
5000 |
150 |
34 |
42 |
35 |
9 |
15 |
- |
Positive controls |
AF-2 |
ENNG |
AF-2 |
AF-2 |
9AA |
4NQO |
Mean No. of colonies/plate ± SD |
501 ± 84.7 |
1101 ± 683.1 |
1082 ± 293.7 |
278 ± 64.8 |
889 ± 275.7 |
270 ± 66.2 |
|
+ |
vehicle control (DMSO) |
154 ± 17.5 |
15 ± 7.1 |
34 ± 10.9 |
42 ± 10.1 |
22 ± 6.3 |
28 ± 5.7 |
+ |
1 |
179 |
15 |
58 |
33 |
15 |
27 |
+ |
5 |
161 |
17 |
56 |
37 |
20 |
24 |
+ |
10 |
174 |
19 |
52 |
46 |
18 |
26 |
+ |
50 |
162 |
19 |
40 |
35 |
15 |
23 |
+ |
100 |
156 |
23 |
49 |
42 |
16 |
30 |
+ |
500 |
158 |
14 |
55 |
39 |
18 |
27 |
+ |
1000 |
184 |
20 |
50 |
46 |
15 |
23 |
+ |
5000 |
177 |
19 |
61 |
31 |
17 |
30 |
+ |
Positive controls |
B(a)P |
2AA |
2AA |
B(a)P |
B(a)P |
B(a)P |
Mean No. of colonies/plate ± SD |
1084 ± 236.3 |
440 ± 198.6 |
359 ± 127.0 |
809 ± 108.4 |
313 ± 41.6 |
354 ± 89.4 |
|
AF-2 = 2-(2 -furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide)
ENNG = N-ethyl-N´-nitro-N-nitrosoguanidine
9AA = 9-aminoacridine
4NQO = 4-nitroquinoline-1-oxide
B(a)P = benzo(a)pyrene
2AA = 2-aminoanthracene
SD = standard deviation
Applicant's summary and conclusion
- Conclusions:
- The test item, adipic acid (CAS 124-04-9), was tested for bacterial mutagenicity according to OECD 471, with S. typhimurium strains TA 1535, 1537, 98, 100 and 1538 as well as E. coli WP2 uvr A at concentration levels up to 5000 µg/plate with and without metabolic activation. Based on the results of the conducted study the test item did not exhibit mutagenic properties in bacterial cells.
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