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EC number: 831-167-5 | CAS number: 2126827-44-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12th February 2019 - 28th February 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Recommended test according to the OECD TG.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Adopted July 21, 1997
- Deviations:
- yes
- Remarks:
- 160 and 500 µL of a dilution of the substance in DMSO was added to the top agar to reach concentrations of 1600 and 5000 µg/plate, respectively. Due to the poor solubility of the substance. The amount of DMSO is of no influence on the results.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- Official Journal of the European Union No. L142, 31 May 2008
- Deviations:
- yes
- Remarks:
- 160 and 500 µL of a dilution of the substance in DMSO was added to the top agar to reach concentrations of 1600 and 5000 µg/plate, respectively. Due to the poor solubility of the substance. The amount of DMSO is of no influence on the results.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,9,16,23-tetrakis(2,5-di-tert-butyl-4-methoxyphenoxy)phthalocyanine
- EC Number:
- 831-167-5
- Cas Number:
- 2126827-44-7
- Molecular formula:
- C92H106N8O8
- IUPAC Name:
- 2,9,16,23-tetrakis(2,5-di-tert-butyl-4-methoxyphenoxy)phthalocyanine
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Batch (Lot) Number: 2715/18/01
Expiry date: 01 July 2020 (expiry date)
Physical Description: Dark green solid
Purity/Composition: 99%
Storage Conditions: At room temperature protected from light
Test item handling: Use amber glassware or wrap container in aluminum foil
Method
- Target gene:
- Strain: Histidine mutation: Mutation type:
TA1537 hisC3076 Frameshift
TA98 hisD3052/R-factor* Frameshift
TA1535 hisG46 Base-pair substitutions
TA100 hisG46/R-factor* Base-pair substitutions
WP2uvrA - Excision repair system deletion
*: R-factor = plasmid pKM101 (increases error-prone DNA repair).
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- other: Each srtarain contains additional mutations: rfa: deep rough (defective lipopolysaccharide cellcoat) gal: mutation in the galactose metabolism chl: mutation in nitrate reductase bio: defective biotin synthesis uvrB: loss of the excision repair system
- Metabolic activation:
- with and without
- Metabolic activation system:
- Microsomal enzymes (S9 homogenate) obtained from male Sprague Dawley rat injected with Aroclor 1254 (500 mg/kg body weight).
- Test concentrations with justification for top dose:
- The substance initially tested in the dose-range finding study exposing TA100 and WP2uvrA tester strains (with and without S9) to the following substance dose: 1.7, 5.4, 17, 52, 164, 512, 1600 and 5000 μg/plate. Based on the results of the dose-range finding test, the following concentrations were chosen for the main experiment: 5.4, 17, 52, 164, 512 and 1600 μg/plate.
- Vehicle / solvent:
- DMSO and saline
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- sodium azide
- methylmethanesulfonate
- other: ICR-191
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- Experimental Design:
Dose-range Finding Test.
Selection of an adequate range of doses was based on a dose-range finding test with the strains TA100 and WP2uvrA, both with and without 5% (v/v) S9-mix. Eight concentrations, 1.7, 5.4, 17, 52, 164, 512, 1600 and 5000 µg/plate were tested in triplicate. The highest concentration of the substance used in the subsequent mutation assays was the level at which the substance exhibited limited solubility.
Mutation Assay.
At least five different doses (increasing with approximately half-log steps) of the substance were tested in triplicate in each strain. The above mentioned dose-range finding study with the two tester strains TA100 and WP2uvrA, is reported as a part of the first mutation experiment. In the second part of this experiment, the substance was tested both in the absence and presence of 5% (v/v) S9-mix in the tester strains TA1535, TA1537 and TA98. In a follow-up experiment with additional parameters, the substance was tested both in the absence and presence of 10% (v/v) S9-mix in all tester strains.
The negative control (vehicle) and relevant positive controls were concurrently tested in each strain in the presence and absence of S9-mix.
Top agar in top agar tubes was melted by heating to 45 ± 2°C. The following solutions were successively added to 3 mL molten top agar: 0.1 mL of a fresh bacterial culture (109 cells/mL) of one of the tester strains, 0.1 to 0.5 mL of a dilution of the substanse in DMSO and either 0.5 mL S9-mix (in case of activation assays) or 0.5 mL 0.1 M phosphate buffer (in case of non-activation assays). The ingredients were mixed on a Vortex and the content of the top agar tube was poured onto a selective agar plate. After solidification of the top agar, the plates were inverted and incubated in the dark at 37.0 ± 1.0 °C for 48 ± 4 h. After this period revertant colonies (histidine independent (His+) for Salmonella typhimurium bacteria and tryptophan independent (Trp+) for Escherichia coli) were counted.
Colony Counting.
The revertant colonies were counted automatically with the Sorcerer Colony Counter. Plates with sufficient substance precipitate to interfere with automated colony counting were counted manually. Evidence of substance precipitate on the plates and the condition of the bacterial background lawn were evaluated when considered necessary, macroscopically and/or microscopically by using a dissecting microscope. - Evaluation criteria:
- A Salmonella typhimurium reverse mutation assay and/or Escherichia coli reverse mutation assay is considered acceptable if it meets the following criteria:
a) The vehicle control and positive control plates from each tester strain (with or without S9-mix) must exhibit a characteristic number of revertant colonies when compared against relevant historical control data generated at Charles River Den Bosch.
b) The selected dose-range should include a clearly toxic concentration or should exhibit limited solubility as demonstrated by the preliminary toxicity range-finding test or should extend to 5 mg/plate.
c) No more than 5% of the plates are lost through contamination or some other unforeseen event. If the results are considered invalid due to contamination, the experiment will be repeated. All results presented in the tables of the report are calculated using values as per the raw data rounding procedure and may not be exactly reproduced from the individual data presented.
A substance is considered negative (not mutagenic) in the test if:
a) The total number of revertants in the tester strain TA100 or WP2uvrA is not greater than two times the concurrent vehicle control, and the total number of revertants in tester strains TA1535, TA1537 or TA98 is not greater than three times the concurrent vehicle control.
b) The negative response should be reproducible in at least one follow-up experiment.
A substance is considered positive (mutagenic) in the test if:
a) The total number of revertants in the tester strain TA100 or WP2uvrA is greater than two times the concurrent vehicle control, or the total number of revertants in tester strains TA1535, TA1537, TA98 is greater than three times the concurrent vehicle control.
b) In case a follow up experiment is performed when a positive response is observed in one of the tester strains, the positive response should be reproducible in at least one follow up experiment.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- In all the experiments the bacterial background lawn was not reduced at any of the concentrations tested and no biologically relevant decrease in the number of revertants was observed.
Dose-range test: Precipitation of the substance on the plates was observed at the start of the incubation period at concentrations of 512 μg/plate and upwards. At the end of the incubation period the substance precipitated at 512 μg/plate and above in tester strains TA100 and WP2uvrA and at 164 μg/plate and above in tester strains TA98, TA1535 and TA1537.
Second mutation experiment: Precipitation of the substance on the plates was observed at the start and at the end of the incubation period at concentrations of 200 μg/plate and upwards. - Remarks on result:
- other: Not mutagenic
- Remarks:
- up to 5000 ug/plate
Any other information on results incl. tables
Dose-Range Finding Test: Mutagenic Response of the test item in the Salmonella typhimurium Reverse Mutation Assay and in the Escherichia coli Reverse Mutation Assay.
Without metabolic activation.
Substance concentration (ug/plate) | TA100 | +/- SD | WP2uvrA | +/-SD |
0 (DMSO 100 uL) | 113 | 11 | 45 | 0 |
0 (DMSO 500 uL) | 102 (nNP) | 10 | 38 (nNP) | 7 |
1.72 | 99 | 2 | 36 | 2 |
5.42 | 88 | 4 | 36 | 5 |
172 | 97 | 15 | 38 | 8 |
522 | 110 | 11 | 37 | 3 |
1642 | 96 (NP) | 15 | 36 (NP) | 5 |
5122 | 99 (SP) | 6 | 37 (SP) | 7 |
16003 |
103 (SP) | 19 | 33 (SP) | 2 |
50004 | 105 (nSP) | 3 | 49 (nSP) | 12 |
Positive control | 848 | 40 | 1668 | 72 |
With metabolic activation1.
Substance concentration (ug/plate) | TA100 | +/- SD | WP2uvrA | +/-SD |
0 (DMSO 100 uL) | 103 | 10 | 38 | 8 |
0 (DMSO 500 uL) | 113 (nNP) | 13 | 44 (nNP) | 10 |
1.72 | 97 | 1 | 35 | 3 |
5.42 | 107 | 13 | 48 | 9 |
172 | 105 | 9 | 44 | 13 |
522 | 100 | 3 | 48 | 5 |
1642 | 113 (NP) | 7 | 45 (NP) | 7 |
5122 | 105 (SP) | 20 | 41 (SP) | 6 |
16003 | 116 (SP) | 28 | 48 (SP) | 13 |
50004 | 108 (nSP) | 17 | 51 (nSP) | 6 |
Positive control | 1374 | 430 | 472 | 27 |
1 Plate incorporation assay (5% S9)
2 100 μL DMSO
3 160 μL DMSO
4 500 μL DMSO
NP No precipitate
SP Slight Precipitate
n Normal bacterial background lawn
Experiment 1: Mutagenic Response of the test item in the Salmonella typhimurium Reverse Mutation Assay
Without metabolic activation.
Substance concentration (ug/plate) | TA1535 | +/- SD | TA1537 | +/-SD | TA98 | +/-SD |
0 (DMSO 100 uL) | 10 | 5 | 5 | 2 | 11 | 3 |
0 (DMSO 160 uL) | 9 (nNP) | 4 | 5 (nNP) | 2 | 12 (nNP) | 2 |
5.42 | 12 | 5 | 4 | 1 | 10 | 1 |
172 | 15 | 3 | 5 | 0 | 14 | 2 |
522 | 11 (NP) | 2 | 6 (NP) | 3 | 16 (NP) | 3 |
1642 | 13 (SP) | 8 | 5 (SP) | 2 | 15 (SP) | 4 |
5122 | 14 (SP) | 2 | 5 (SP) | 3 | 16 (SP) | 5 |
16003 |
15 (nSP) |
3 | 6 (nSP) | 3 | 12 (nSP) | 4 |
Positive control | 1009 | 31 | 1100 | 135 | 1317 | 95 |
With metabolic activation.
Substance concentration (ug/plate) | TA1535 | +/- SD | TA1537 | +/-SD | TA98 | +/-SD |
0 (DMSO 100 uL) | 15 | 4 | 5 | 3 | 18 | 3 |
0 (DMSO 160 uL) | 11 (nNP) | 7 | 4 (nNP) | 1 | 23 (nNP) | 4 |
5.42 | 8 | 3 | 6 | 1 | 12 | 3 |
172 | 11 | 2 | 6 | 2 | 13 | 5 |
522 | 8 (NP) | 3 | 4 (NP) | 4 | 17 (NP) | 4 |
1642 | 9 (SP) | 2 | 4 (SP) | 1 | 15 (SP) | 9 |
5122 | 8 (SP) | 4 | 7 (SP) | 3 | 10 (SP) | 5 |
16003 |
9 (nSP) | 2 | 6 (nSP) | 2 | 14 (nSP) | 3 |
Positive control | 296 | 12 | 210 | 20 | 1189 | 298 |
1 Plate incorporation assay (5% S9)
2 100 μL DMSO
3 160 μL DMSO
NP No precipitate
SP Slight Precipitate
n Normal bacterial background lawn
Experiment 2: Mutagenic Response of the test item in the Salmonella typhimurium Reverse Mutation Assay and in the Escherichia coli Reverse Mutation Assay
Without metabolic activation.
Substance concentration (ug/plate) | TA1535 ( +/- SD) | TA1537 (+/- SD) |
TA98 ( +/- SD) |
TA100 ( +/- SD) |
WP2uvrA ( +/- SD) |
0 (DMSO 100 uL) | 11 (1) | 3 (1) | 6 (4) | 91 (20) | 30 (9) |
52 | 10 (2) | 5 (5) | 10 (3) | 101 (18) | 34 (3) |
102 | 9 (5) | 1 (1) | 8 (6) | 96 (9) | 29 (8) |
502 | 12 (8) NP | 3 (2) NP | 16 (2) NP | 118 (6) NP | 28 (3) NP |
2002 | 6 (5) SP | 3 (3) SP | 19 (1) SP | 100 (2) SP | 28 (7) SP |
5002 | 9 (2) SP | 4 (4) SP | 10 (4) SP | 101 (11) SP | 31 (3) SP |
10002 | 11 (6) nSP | 2 (2) nSP | 7 (4) nSP | 93 (3) nSP | 37 (5) nSP |
Positive control | 1013 (6) | 1168 (58) | 1153 (110) | 913 (41) | 1698 (54) |
With metabolic activation.
Substance concentration (ug/plate) | TA1535 ( +/- SD) | TA1537 (+/- SD) |
TA98 ( +/- SD) |
TA100 ( +/- SD) |
WP2uvrA ( +/- SD) |
0 (DMSO 100 uL) | 14 (2) | 9 (2) | 20 (7) | 98 (15) | 46 (15) |
52 | 12 (4) | 3 (2) | 19 (4) | 79 (7) | 35 (2) |
102 | 13 (3) | 4 (2) | 17 (3) | 85 (8) | 35 (13) |
502 | 14 (9) NP | 7 (6) | 15 (6) NP | 78 (4) NP | 33 (8) NP |
2002 | 12 (4) SP | 9 (3) SP | 22 (8) SP | 75 (10) SP | 30 (5) SP |
5002 | 14 (2) SP | 6 (5) SP | 18 (8) SP | 85 (12) SP | 37 (3) SP |
10002 | 13 (4) nSP | 6 (3) nSP | 18 (4) nSP | 84 (4) nSP | 38 (0) nSP |
Positive control | 313 (16) | 452 (16) | 559 (22) | 1512 (363) | 340 (23) |
1 Plate incorporation assay (10% S9)
2 100 μL DMSO
NP No precipitate
SP Slight Precipitate
n Normal bacterial background lawn
Applicant's summary and conclusion
- Conclusions:
- The substance is not mutagenic in the Salmonella typhimurium and the Escherichia coli reverse mutation assay in the presence and absence of metabolic activation.
- Executive summary:
The potential of the substance to induce reverse mutations at the histidine locus in several strains of Salmonella typhimurium (TA98, TA100, TA1535, and TA1537), and at the tryptophan locus of Escherichia coli strain WP2uvrA was evaluated in the presence or absence of an exogenous mammalian metabolic activation system (S9). The study was conducted in accordance with OECD TG 471 ‘Bacterial Reverse Mutation Assay’.
Prior to the main study a dose-range finding test was conducted on S. typhimurium TA100 and E.coli WP2urvA strains (with and without metabolic activation system), which were exposed to the following concentrations of the substance: 0, 1.7, 5.4, 17, 52, 164, 512, 1600 and 5000 µg/plate (in triplicate). The bacterial background lawn was not reduced at any of the concentrations tested and no biologically relevant decrease in the number of revertants was observed.
The first mutation assay was performed using the following concentrations of the substance 0, 5.4, 17, 52, 164, 512 and 1600 µg/plate (in triplicate) in the absence and presence of 5% (v/v) S9-mix in the tester strains TA1535, TA1537 and TA98.The bacterial background lawn was not reduced at any of the concentrations tested and no biologically relevant decrease in the number of revertants was observed.
The second mutation assay was performed using the following concentrations of the substance: 0, 5, 10, 50, 200, 500 and 1000 µg/plate (in triplicate) in the absence and presence of 10% (v/v) S9-mix in the tester strains TA1535, TA1537, TA98, TA100 and WP2uvrA. The bacterial background lawn was not reduced at any of the concentrations tested and no biologically relevant decrease in the number of revertant colonies was observed.
Based on the results of this study it is concluded that the substance is not mutagenic in the Salmonella typhimurium and the Escherichia coli reverse mutation assay in the presence and absence of metabolic activation.
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