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EC number: 811-285-3 | CAS number: 1637294-12-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 May, 2015 to 05 October, 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 23 March, 2006, with updated Annex V of 28 July, 2011.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- NYMPHEAL (GR-88-0778) :
Lot No. : Batch 6
Purity : 99.1% (sum of isomers)
Date of Expiry : 29 March, 2017 - Analytical monitoring:
- yes
- Remarks:
- GC-MS
- Details on sampling:
- Water samples were taken for extraction and analysis, as follows :
- Exposure samples (C1 - C5) - Time 0, 48 hours, 72 hours
- Control and Solvent Control - Time 0 and 72 hours
- Stability samples (without the presence of algae, and, without the presence of algae and light) at the intermediate test concentration C3 (nominal 1.03 mg/L) - analysed following 48 and 72 hours of incubation. - Vehicle:
- yes
- Remarks:
- Stock solutions prepared in Acetone. Maximum loading in exposure media : 0.1 mL/L.
- Details on test solutions:
- Following a Range-Finding study, the Following Nominal test concentrations were chosen for the definitive study :
C1 0.21 mg/L
C2 0.47 mg/L
C3 1.03 mg/L
C4 2.27 mg/L
C5 5.00 mg/L
The exposure solutions were prepared using standards prepared in Acetone as a vehicle. Since NYMPHEAL is a primary aldehyde, and, therefore, susceptible to undergo rapid oxidation, it was decided to avoid long, drawn-out exposure medium preparation techniques (e.g. slow-stirring to obtain water saturated fraction).
A blank Control and Solvent Control were also performed. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Desmosdesmus subspicatus
Pre-Culture Conditions :
Pre-Culture Vessels : 300 mL Erlenmeyer flasks
Culture Medium : Modified OECD Medium (OECD 201, EN ISO 8692)
pH value of algal medium : 8.2
Volume of liquid for Stock Culture : 100 +/- 5 mL
Number of Replicates : 2
Light : Permanently illuminated
Light Intensity : 60 - 120 microE/m2/s
Shaker : 100 +/- 5 oscillations/minute
Temperature : 21 - 24 °C (+/- 2°C) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22 °C (21.5 - 22.9 °C)
- pH:
- pH of test medium : 8.3
pH in test solutions : 8.5 - 9.1 - Nominal and measured concentrations:
- The water samples were simultaneously analysed for the residue of the Parent, and the corre-sponding alcohol (GR-88-1108) and acid (GR-88-1910).
NYMPHEAL was not stable in the exposure solutions over the 24-hour renewal period. The concentrations determined in the Fresh medium samples at Time 0 varied in the range of 81 to 107 % of Nominal, and, between less than LOQ - 91% of Nominal in the 48 hour old samples, decreasing further by 72 hours.
The Time-Weighted Mean Measured concentrations of the Parent over the 96-hour exposure period were, as follows :
Exposure Level Time-Weighted Mean Concn.
Control Not Detected
Solvent Control Not Detected
C1 (0.21 mg/L) 0.052 mg/L
C2 (0.47 mg/L) 0.123 mg/L
C3 (1.03 mg/L) 0.475 mg/L
C4 (2.27 mg/L) 1.30 mg/L
C5 (5.00 mg/L) 3.62 mg/L
By 72 hours, detects of the Acid Metabolite represented more-or-less the totality of Nympheal équivalents detected.
An average of 83% of Nympheal was recovered after 72 hours of incubation of the stability control samples (i.e. C3 without algae, and C3 without algae and no light). - Details on test conditions:
- Test Vessels : 60 mL glass vials, air-tightly closed bÿ a screw-cap with PTFE lining
Volume of Exposure Medium : 60 +/- 2 mL
Age of Pre-Culture : 4 days
Number of Cells per mL of Test Solution : 5000 cells/mL
Number of Replicates per test item concentration : 3
Number of Replicates in the Control : 3
Number of Replicates in the Solvent Control : 6
Light Regime : 24 hours of light
Light Intensity : 68.2 - 78.4 microS/cm2/s
Temperature : 21.5 - 22.9 °C
Shaker : 100 +/- 5 oscillations/minute
Test Duration : 72 hours
Counting of Algae : Daily - Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.55 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% Confidence Interval Limits : 1.47 - 1.62 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.561 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% Confidence Interval Limits : 0.502 - 0.617 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.123 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: NOEC for Growth Rate and Yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.719 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 95% Confidence Interval Limits : 0.675 - 0.766 mg/L
- Results with reference substance (positive control):
- A reference test using Potassium Dichromate as reference item was performed in December 2014.
Result : ErC50 (0 - 72 h) = 0.940 mg/L (95% CI 0.900 - 0.983 mg/L)
This result is in accordance with the range provided in the international ring test (ISO, 2004). - Validity criteria fulfilled:
- yes
- Conclusions:
- An algal growth inhibition test with Desmodesmus subspicatus was performed with NYMPHEAL. The corresponding end-points at 72 hours of incubation are :
72 hr ErC50 = 1.55 mg/L
72 hr ErC10 = 0.561 mg/L
72 hr NOErC = 0.123 mg/L
72 hr EyC50 = 0.719 mg/L
72 hr EyC10 = 0.305 mg/L
72 hr NOEyC = 0.123 mg/L - Executive summary:
An algal growth inhibition test with Desmodesmus subspicatus was performed with NYMPHEAL. The corresponding end-points at 72 hours of incubation are :
72 hr ErC50 = 1.55 mg/L
72 hr ErC10 = 0.561 mg/L
72 hr NOErC = 0.123 mg/L
72 hr EyC50 = 0.719 mg/L
72 hr EyC10 = 0.305 mg/L
72 hr NOEyC = 0.123 mg/L
Reference
Description of key information
OECD 201 - Algal Inhibition :
ErC50 (72 hr) = 1.55 mg/L
ErC10 (72 hr) = 0.561 mg/L
NOErC (72 hr) = 0.123 mg/L
EyC50 (72 hr) = 0.719 mg/L
EyC10 (72 hr) = 0.305 mg/L
NOEyC (72 hr) = 0.123 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.55 mg/L
- EC10 or NOEC for freshwater algae:
- 0.561 mg/L
Additional information
OECD 201 - Algal Inhibition :
ErC50 (72 hr) = 1.55 mg/L
ErC10 (72 hr) = 0.561 mg/L
NOErC (72 hr) = 0.123 mg/L
EyC50 (72 hr) = 0.719 mg/L
EyC10 (72 hr) = 0.305 mg/L
NOEyC (72 hr) = 0.123 mg/L.
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