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EC number: 700-541-9 | CAS number: 1472634-24-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 OCT 2012 - 07 MAY 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 July 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Gewerbeaufsichtsamt Hildesheim (5 November 2013)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was warmed-up to 30 °C for 30 minutes. Afterwards the test item was weighed out directly in Erlenmeyer flasks, filled up with 200 mL demineralised water and stirred overnight (450 upm).
- Controls: blank control (test medium without test or reference item), positive control (reference item: Copper (II) sulphate pentahydrate) - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Municipal sewage treatment plant of 31137 Hildesheim, Germany
- Preparation of inoculum for exposure/ Pretreatment: The sludge was washed twice with tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The sludge was used within 24 h after sampling. - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- demineralised water
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- none
- Test temperature:
- 21.1 °C
- pH:
- pH-value of the activated sludge: 7.43
pH-value of the synthetic waste water: 7.45 - Nominal and measured concentrations:
- Nominal concentrations 100, 320, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 m L Erlenmeyer flasks, DIN 12380
- Material, size, headspace, fill volume: glass, 500 mL, DIN 12380
- Aeration: Permanent, to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension
- No. of vessels per concentration (replicates): 5 replicates per concentration
- No. of vessels per control (replicates): Triplicates
- No. of vessels per abiotic control (replicates): Triplicates per concentration
- Sludge concentration (weight of dry solids per volume): dry sludge concentration 3.17 g/L, corresponding to 1.59 g/L suspended solids in the test vessel
- Nutrients provided for bacteria: 16 mL Synthetic waste water acc. to OECD TG 209
- Nitrification inhibitor used: none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Demineralised water
OTHER TEST CONDITIONS
- Adjustment of pH: The pH value of the activated sludge was determined prior to test start, the pH value of the synthetic waste water was determined prior to use. Adjustment to 7.5 ± 0.5 was not necessary.
EFFECT PARAMETERS MEASURED: NOEC, EC50
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study : Concentrations were based on a non-GLP range finding test (100 - 500 - 1000 mg/L).
- Test concentrations: 100 - 320 - 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate was used as reference item and the reference toxicity was determined.
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: No statistically significant effect (p < 0.05), ANOVA, Dunnett's Method
- Details on results:
- - Blank controls oxygen uptake rate:
34.5 mg 02/g·h
- Coefficient of variation of oxygen uptake rate in control replicates: 8.1 %
for detailed results please refer to attached table under 'Attached background material' - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3h) = 104 mg/L (95% Confidence lnterval = 102 - 107 mg/L). This is in the recommended range of validity of 53 - 155 mg/L. - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC50-value of the reference item was calculated by linear regression using software GraphPad Prism. Calculation of the confidence intervals for the EC-values was carried out using standard procedures. - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item is 320 mg/L.
The test item is not toxic up to the concentration of 320 mg/L to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item in compliance with GLP. Test system was activated sludge of a municipal treatment plant. The test was carried out under static conditions with the substance concentrations of 100 - 320 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates were in the range of 2 % to 36 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 104 mg/L. The NOEC of the test item is 320 mg/L.
The test item is not toxic up to the concentration of 320 mg/L to activated sludge of a municipal sewage treatment plant.
Reference
Description of key information
NOEC (3h) = 320 mg/L and EC50 (3h) > 1000 mg/L for activated sludge based on respiration inhibition (OECD 209, GLP)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 320 mg/L
Additional information
Respiration inhibition test with activated sludge, OECD 209
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item in compliance with GLP. Test system was activated sludge of a municipal treatment plant. The test was carried out under static conditions with the substance concentrations of 100 - 320 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates were in the range of 2 % to 36 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50 value for the reference item was 104 mg/L.
The NOEC of the test item is 320 mg/L. The test item is not toxic to activated sludge of a municipal sewage treatment plant up to the concentration of 320 mg/L.
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