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EC number: 430-710-1 | CAS number: 15290-77-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 1997-10-22 to 1998-01-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 4 April 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: 9705-2A
Purity: 98.18% - Analytical monitoring:
- yes
- Details on sampling:
- In order to assess the exposure concentration of test item, independently prepared control mixtures (nominal concentration, 100 mg/L) containing the test material, injected into dilution water alone, were established under test conditions. Samples for analysis (40 mL) were removed before and after the three-hour aeration period.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate volumes were injected, using a microsyringe, into test beakers containing dilution water maintained at the test temperature. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
maintained under aerobic conditions until required.
- Name and location of sewage treatment plant where inoculum was collected: Oakley sewage treatment works
- Preparation of inoculum for exposure: On the day of collection, aliquots (25 mL) of the activated sludge were filtered through dried and preweighed Whatman's GFC filter papers which were then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated and adjusted to 4g/L by the removal of supernatant. Synthetic sewage (50 mL/L) was added and the mixture aerated overnight.
- Initial biomass concentration: 4g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- 60 mg/L
- Test temperature:
- 19.0-19.9 °C
- pH:
- 7.1-8.1
- Nominal and measured concentrations:
- Nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: one-litre glass beakers, fill volume: Ca. 500 mL
- Aeration: aerated using a Pasteur pipette connected to a laboratory supply of oil-free compressed air
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 1.6 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water used to prepare stock solutions of the test material was tap water that had been softened and treated by reverse osmosis (Elga Ltd, Prima 4 reverse osmosis unit) and then purified (Elga Ltd, UHP) to give a conductivity of 18 Megohm/cm.
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED:
Following the exposure period, a well-mixed sample of each mixture was transferred to a biochemical oxygen demand (BOD) bottle (capacity; 270 mL) and its rate of oxygen consumption over a period of approximately ten minutes was measured using a Yellow Springs Instruments (YSI) dissolved oxygen meter, with temperature probe and self-stirring bottle probe, connected to a chart recorder. The pH and temperature ofthe samples were measured at the start and end of the test. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Inhibitory effect: No significant inhibitory effect on the respiration rate of activated sludge at an initial nominal concentration of 100 mg/L. Respiration rates in mixtures containing test item were reduced, at most, by 5% of the mean control value.
- Blank controls oxygen uptake rate: 30.0 mgO2/g/hr at the end of the test was 102% of the rate of that established at the start (29.3 mgO2/g/hr).
- Coefficient of variation of oxygen uptake rate in control replicates: < 15% - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 3h-EC50: 8.8 mg/L (95% confidence limits 7.1 - 10.8 mg/L)
- Other: acceptable EC50 range 5 to 30 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had no significant inhibitory effect on the respiration rate of activated sludge. The EC20, EC50 and EC80 of the test material could not therefore be calculated but these must be greater than 100 mg/L.
- Executive summary:
The effect of test item on the respiration rate of activated sludge was assessed by the methods detailed in OECD Test Guideline 209.
Triplicate samples of activated sludge (suspended solids 1.6 g/L), fed with synthetic sewage, were exposed to the test material at a nominal level of 100 mg/L for three hours.
The measured levels of test item at the start and end of the aeration period (25.4 and 0 mg/L respectively) indicated that material had not completely dissolved at the start and was readily lost from the mixture over three hours as a result of its volatility.
The test item had no significant inhibitory effect on the respiration rate of activated sludge. The EC20, EC50 and EC80 of the test material could not therefore be calculated but these must be greater than 100 mg/L.
Reference
Levels of test item estimated by Total Carbon analysis and Chemical Oxygen Demand were low (0.01 - 0.02% ofthe nominal level) at both the start and end of the aeration period. Since the material was known to be miscible with water, these results suggest that test item may not have been oxidised under the conditions employed in these analyses.The measured levels of test item at the start and end of the aeration period (25.4 and 0 mg/L respectively) indicated that material had not completely dissolved at the start and was readily lost from the mixture over three hours as a result of its volatility.
Description of key information
The effect of test item on the respiration rate of activated sludge was assessed by the methods detailed in OECD Test Guideline 209.
The 3h EC20, EC50 and EC80 of the test material could not therefore be calculated but these must be greater than 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
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