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EC number: 202-987-5 | CAS number: 101-90-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- This study was accepted for publication on 17 October 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Reliability 2 was assigned as the study was conducted to a recognised procedure it is a comparative study with no clear endpoint data.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The micronucleus test was performed in mice according to the established procedures (Schmid, 1976; Heddle and Salamone, 1981). Male and female mice were given the test compounds orally, dissolved in polyethyleneglycol (PEG 400), in doses up to acutely toxic levels. 24 h after this single dose, the mice were sacrificed and the bone marrow cells were flushed out into foetal calf serum. In the case of a negative outcome of the test, a second assay was performed with fixation times of 24, 48, and 72 h, resp. After centrifugation at 400 g, the cells were spread onto slides, air-dried and stained with May-Grünwald/Giemsa. In this case, the slides were coded and analysed by two individuals separately.
- GLP compliance:
- no
- Type of assay:
- other: Chromosomal aberration
Test material
- Reference substance name:
- m-bis(2,3-epoxypropoxy)benzene
- EC Number:
- 202-987-5
- EC Name:
- m-bis(2,3-epoxypropoxy)benzene
- Cas Number:
- 101-90-6
- Molecular formula:
- C12H14O4
- IUPAC Name:
- 2-({3-[(oxiran-2-yl)methoxy]phenoxy}methyl)oxirane
- Test material form:
- liquid: viscous
Constituent 1
- Specific details on test material used for the study:
- Resorcinoi diglycidyl ether (RDGE) was synthesized according to pub- lished methods (Ross et al., 1964); briefly, the parent compounds, N-methylaniline, aniline, and resorcinol, resp., were first reacted with an approximately 10-fold excess of epichloro- hydrin at slightly elevated temperatures. Upon completion of the reaction, a 50% sodium hydrox- ide solution was added slowly over a period of 1-3h in an amount equivalent to the number of reactive sites. The product was finally dissolved in toluene, washed with water and dried over anhydrous sodium sulfate. The solvent was then removed in vacuo and the product was purified by vacuum distillation. The purified product was analyzed by HPLC (on a silica column, with the same solvent as above) and was estimated to contain less than 2% impurities. The alkylating potency of the epoxide was measured in the 4-(4-nitro-benzyl)pyridine (NBP) assay according to Friedman and Boger (1961).
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Mice of the ICR-strain were obtained from the Institute of Animal Husbandry of the University of Zurich
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Polyethylene glycol (PEG 400)
- Details on exposure:
- Male and female mice of body weight circa 25 g, were given the test compounds orally, dissolved in polyethylene-glycol (PEG 400), in doses up to acutely toxic levels.
- Duration of treatment / exposure:
- once
- Frequency of treatment:
- once
- Post exposure period:
- 24h
Doses / concentrationsopen allclose all
- Dose / conc.:
- 300 mg/kg bw (total dose)
- Remarks:
- first assay: 24 after single dose
- Dose / conc.:
- 600 mg/kg bw (total dose)
- Remarks:
- second assay was performed with fixation times of 24, 48 and 72 h
- No. of animals per sex per dose:
- 4 males and 4 females
- Control animals:
- no
Examinations
- Tissues and cell types examined:
- polychromatic erythrocytes from the bone marrow
- Details of tissue and slide preparation:
- 24 h after this single dose, the mice were sacrificed and the bone marrow cells were flushed out into foetal calf serum. In the case of a negative outcome of the test, a second assay was performed with fixation times of 24, 48, and 72 h, resp. After centrifugation at 400 g, the cells were spread onto slides, air-dried and stained with May-Grtinwald/ Giemsa.
- Evaluation criteria:
- Not specified
- Statistics:
- There the standard deviation of the frequency of micronucleated erythrocytes (per thousand polychromatic erythrocytes) was calculated. The spontaneous frequency of micronucleated polychromatic erythrocytes (3-5 per thousand polychromatic erythrocytes, standard deviation 1.8) has been subtracted.
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- one out of four mice died within 48 h in each group
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Mice were given solutions of the test compound orally in dosages up to the LD50, and the polychromatic erythrocytes from the bone marrow of such treated animals were checked for the presence of micronuclei. The in vitro much more active RDGE, on the other hand, proved to be completely inactive. The inability of RDGE to induce micronuclei in the bone marrow of mice in vivo could not be traced to some influence on the length of the cell cycle, since the compound was also inactive at 48 and 72 h after dosage, nor was the dose given insufficient, since 1 out of 4 mice died within 48 h in each experimental group.
Any other information on results incl. tables
Table 1 INDUCTION OF MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES 1N THE BONE MARROW OF MICE TREATED WITH RESORCINOL DIGLYCIDYL ETHER
Fixation Time (h) | Frequency (and standard deviation) of micronucleated erythrocytes (per thousand polychromatic erythrocytes) at dose (mg/kg p.o.) of |
||||||
300 | 400 | 500 | 600 | 800 | 1000 | 1500 | |
24 | 0.0 (1.5) | 0.0 (1.9) | |||||
48 | 0.9 (1.3) | ||||||
72 | 0.4 (0.6) |
The spontaneous frequency of micronucleated polychromatic erythrocytes (3-5 per thousand polychromatic erythrocytes, standard deviation 1.8) has already been subtracted.
Applicant's summary and conclusion
- Conclusions:
- The article has demonstrated that resorcinol diglycidyl ether (RDGE) was completely inactive in vivo micronucleus assay in mice.
- Executive summary:
The micronucleus test was performed in male and female mice of the ICR-strain. The test item resorcinol diglycidyl ether (RDGE) dissolved in polyethylene-glycol (PEG 400) was given orally in doses up to acutely toxic level. 24 h after the single dose (300 mg/kg po), the mice were sacrificed and the bone marrow cells were flushed out into foetal clf serum. In the case of negative outcome of the test, a second assay was performed with fixation times of 24, 48 and 72 h, respectively. After centrifugation at 400 g, the cells were spread onto slides, air-dried and stained with May-Grünwald/Giemsa. The slides were coded and analysed by two individuals separately. The polychromatic erythrocytes from the bone marrow of treated animals were checked for the presence of micronuclei. RDGE proved to be completely inactive. The inability of RDGE to induce micronuclei in the bone marrow of mice in vivo could not be traced to some influence on the length of the cell cycle, since the compound was also inactive at 48 and 72 h after dosage, nor was the dose given insufficient, since 1 out of 4 mice died within 48 h.
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