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EC number: 443-010-4 | CAS number: 53641-10-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-01-06 to 2000-03-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Test chambers were aerated from 24 hours after study start, since the maintenance of an oxygen content of at least 60 % of the saturation value was not ensured. Aeration was carried out using a glass capillary with a bubble-frequency of 1 - 3 per second.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 443-010-4
- EC Name:
- -
- Cas Number:
- 53641-10-4
- Molecular formula:
- C14H15ClN2O4
- IUPAC Name:
- N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- In order to determine the substance concentration, water samples were taken from the middle of the test chamber by pipetting approx. 20 mL into a screw-neck glass bottle at study start, and after 48 and 96 hours.
Test solutions
- Vehicle:
- yes
- Remarks:
- Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution. Preparation was carried out in a unit consisting of two Hostalen®- lined steel vessels with a capacity of 1000 litres each. The dilution water was prepared as des
- Details on test solutions:
- A solubilizer was not used. The test substance was weighed
into a beaker, water for dilution was added and was
homogenized using an Ultra-Turrax.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species Danio rerio (HAMILTON-BUCHANAN)
Origin : Aventis Pharma Deutschland GmbH
ProTox
Date of hatching February, 11 1999
Delivery date April, 14 1999
The fish were kept for 14 days before the start of the study in water for dilution (see section 6.3) under the following conditions:
Temperature : 22 ± 1 °C
Oxygen content : 80 % of the saturation value
Duration of light period : 12 hours daily
Fish density 1 g fish / L water
Feeding : twice daily ad libitum
Food : Tetra Min, Tetra Werke, Melle (Germany)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- The total hardness of the dilution water was determined weekly and was at 2.1 mmol Ca2+ + Mg2+ / L during the study.
- Test temperature:
- 21.5-22.1 °C
- pH:
- 7.5-8.2
- Dissolved oxygen:
- 6.1-9.4 mg/L
- Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured: 93 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Details on results:
- The 100 mg/L group showed no effect in comparison with the control. In the control group and in the 100 mg/L group no mortality was observed.
In this 96-hour acute toxicity study of CL-DAEP in zebra fish (Danio rerio) no mortality occurred in the 100 mg/L group (nominal concentration) and in the control group.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Aeration was started from 24 h into to the test, measured concentration of 6.1-9.4 mg/L do not indicate reasons for concern regarding the validity of the results.
- Conclusions:
- Under the conditions of this test the LC50 of CL-DAEP after 96 hours was >100 mg/L.
- Executive summary:
CL-DAEP was tested in zebra fish (Danio rerio) for 96 hours in a static system. The concentration tested was 100 mg/L and a negative control (0 mg/L).
The chamber contents were stirred for approximately 24 hours with a KPG-stirrer and a glass rod to ensure that the limit of solubility of the test substance in water was reached.
The tested concentration was visually present as clear solution. The test batch was homogeneous. No particulate matter was observed.
The substance concentration was determined in the 100 mg/L group. The values determined were in a range of ± 10 % of the theoretical value.
In this 96-hour acute toxicity study of CL-DAEP the following mortality values were determined:LC0 (24, 48, 72, 96 h) = 100 mg/L
LC50 (24, 48, 72, 96 h) > 100 mg/L
LC100 (24, 48, 72, 96 h) > 100 mg/L
The 100 mg/L group showed no effect in comparison with the control. In the control group no effects were observed.
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