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EC number: 833-435-7 | CAS number: 2133415-29-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
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- Toxicological Summary
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Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil ww
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: 95 % Cl not reported
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil ww
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: 95 % CL limits not recorded
- Details on results:
- RESULTS
Range-finding Test
Absorbance values, nitrate concentrations and percentage inhibition of nitrogen transformation activity determined during the range-finding test are given in Table 1.
The results showed no significant effect on nitrogen transformation activity.
Based on this information a single test concentration of 1000 mg/kg soil was selected for the definitive test. This experimental design conforms to a “limit test” to confirm that at the maximum recommended concentration given in the Test Guidelines no effect on nitrogen transformation was observed.
Definitive Test
Absorbance values and nitrate concentrations determined on each sampling day are given in Table 2. The nitrate formation rates and percentage inhibition of nitrogen transformation activity on days 7 and 28 are given in Table 3.
The microbial biomass of the soil was shown to be 134 µg C/g (ISO 14240-2: 1997). This was equivalent to 1.4% of the total soil organic carbon content.
The variation between replicate control nitrification rates was less than 15% and therefore satisfied the validation criterion given in the Test Guidelines.
Accordingly the following results were determined from the data:
EC10 (28 days): >1000 mg/kg
EC25 (28 days): >1000 mg/kg
EC50 (28 days): >1000 mg/kg
where ECx is the test concentration that reduced soil nitrogen transformation activity by x %.
Statistical analysis of the nitrate concentration values was carried out for the control and test item group and showed no statistically significant decreases between the control and 1000 mg/kg test concentration (P≥0.05). Therefore the ‘No Observed Effect Concentration’ (NOEC) was 1000 mg/kg.
Temperature was maintained at approximately 21ºC throughout the test and the moisture content of each control and test vessel was checked on days 7, 14, 21 and 28 to ensure that the moisture content of the soil was maintained to 40% ± 5% of the WHC of the soil.
The mean nitrate formation rate in the range-finding test was significantly higher in the 1000 mg/kg test concentration than in the control; however inthe definitive test the mean nitrate formation rate in the control and 1000 mg/kg test concentration were similar.
The results in Tables 1 and 2 also show a significant increase in the nitrate formation rate between the control and 1000 mg/kg test vessels prepared in the range-finding and definitive test. This increase may be due to the soil having been stored between the range-finding and definitive tests. The difference in nitrogen transformation rates between the range-finding and definitive tests is considered to have had no adverse effect on the study given that in both tests no inhibition of the nitrogen transformation rate between the control and test group was observed. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Statistical analysis
A Students t-test was carried out on the nitrate concentration values on Day 28 for the control and the 1000 mg/kg test concentration to determine any statistically significant differences between the test and control groups. All statistical analyses were performed using the SAS computer software package (SAS 1999 - 2001). - Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the read across substance GTL Base Oil Distillates on the nitrogen transformation activity of soil microorganisms has been investigated over a 28-Day period and gave an EC50 value of greater than 1000 mg/kg. Correspondingly the No Observed Effect Concentration was 1000 mg/kg.
- Executive summary:
Introduction.
A study was performed to assess the long-term effect of the read across substance ‘Distillates (Fischer-Tropsch), heavy, C18-50 - branched, cyclic and linear' after a single exposure, on the nitrogen transformation activity of soil microorganisms. The method followed that described in the OECD Guidelines for Testing of Chemicals (2000) No 216 “Soil Microorganisms: Nitrogen Transformation Test” referenced as Method C.21 of Commission Regulation (EC) No. 440/2008; EPPO (1994) Decision Making Scheme for the Environmental Risk Assessment of Plant Protection Products, Chapter 7: Soil Microflora, EPPO Bulletin 24 and SETAC-Europe (1995) Procedures for Assessing the Environmental Fate and Ecotoxicity of Pesticides.
Methods.
Following a preliminary range-finding test soil microorganisms were exposed to the test item at a single concentration of 1000 mg/kg for 28 days at a temperature of approximately 21 ºC, in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.
The inhibitory effect of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0, 7 and 28 and compared to data obtained from control soil samples.
Results.
The effect of the test item on the nitrogen transformation activity of soil microorganisms has been investigated over a 28-Day period and gave an EC50 value of greater than 1000 mg/kg. Correspondingly the No Observed Effect Concentration was 1000 mg/kg.
CONCLUSION
The effect of the test item on the nitrogen transformation activity of soil microorganisms has been investigated over a 28-Day period and gave an EC50 value of greater than 1000 mg/kg. Correspondingly the No Observed Effect Concentration was 1000 mg/kg.
Reference
Table 1: Absorbance Readings, Nitrate Concentrations and Inhibition of Nitrogen Transformation Activity from the Range-finding Test
Nominal Concentration |
Day 0 |
Nominal Concentration |
Day 28 |
||||||
Absorbance* |
Nitrate Concentration (mg NO3/kg) |
Absorbance* |
Nitrate Concentration (mg NO3/kg) |
Mean Nitrate Formation Rate |
% Inhibition |
||||
Control |
R1 |
0.245 |
88.35 |
Control |
R3 |
0.359 |
128.50 |
1.33 |
1 |
|
R2 |
0.244 |
88.00 |
|
R4 |
0.342 |
122.50 |
||
10 |
R1 |
0.242 |
87.30 |
10 |
R3 |
0.359 |
128.50 |
1.52 |
[14] |
|
R2 |
0.235 |
84.85 |
|
R4 |
0.360 |
128.85 |
||
100 |
R1 |
0.251 |
90.50 |
100 |
R3 |
0.367 |
131.30 |
1.59 |
[20] |
|
R2 |
0.248 |
89.40 |
|
R4 |
0.385 |
137.65 |
||
1000 |
R1 |
0.245 |
88.35 |
1000 |
R3 |
0.615 |
218.55 |
5.01 |
[277] |
|
R2 |
0.248 |
89.40 |
|
R4 |
0.675 |
239.70 |
*Corrected for blank absorbance value
[Increase in nitrate formation rate as compared to controls]
R1-R4= Replicates R1to R4
Table 2: Absorbance and Nitrate Concentrations from the Definitive Test
Nominal Concentration (mg/kg soil) |
Day 0 |
||
Absorbance* |
Nitrate Concentration |
||
Control |
R1 |
0.304 |
109.15 |
|
R2 |
0.295 |
105.95 |
|
R3 |
0.291 |
104.55 |
|
Mean |
- |
106.55 |
|
CV% |
- |
2 |
1000 |
R1 |
0.313 |
112.30 |
|
R2 |
0.306 |
109.85 |
|
R3 |
0.310 |
111.25 |
|
R4 |
0.295 |
105.95 |
|
R5 |
0.306 |
109.85 |
|
R6 |
0.307 |
110.20 |
|
Mean |
- |
109.90 |
|
CV% |
- |
2 |
Nominal Concentration (mg/kg soil) |
Day 7 |
||
Absorbance* |
Nitrate Concentration |
||
Control |
R4 |
0.543 |
193.25 |
|
R5 |
0.530 |
188.65 |
|
R6 |
0.510 |
181.60 |
|
Mean |
- |
187.33 |
|
CV% |
- |
3 |
1000 |
R7 |
0.524 |
186.55 |
|
R8 |
0.554 |
197.10 |
|
R9 |
0.505 |
179.85 |
|
R10 |
0.519 |
184.80 |
|
R11 |
0.534 |
190.05 |
|
R12 |
0.515 |
183.40 |
|
Mean |
- |
186.96 |
|
CV% |
- |
18 |
*Corrected for blank absorbance value
R1-R12= Replicates 1 to 12
CV = Coefficient of Variation
Table 2 (continued): Absorbance Readings and Nitrate Concentrations from the Definitive Test
Nominal Concentration (mg/kg soil) |
Day 28 |
||
Absorbance* |
Nitrate Concentration |
||
Control |
R7 |
1.194 |
422.30 |
|
R8 |
1.188 |
420.20 |
|
R9 |
1.192 |
421.60 |
|
Mean |
- |
421.37 |
|
CV% |
- |
<1 |
1000 |
R13 |
1.192 |
421.60 |
|
R14 |
1.198 |
423.75 |
|
R15 |
1.193 |
421.95 |
|
R16 |
1.175 |
415.65 |
|
R17 |
1.232 |
435.70 |
|
R18 |
1.199 |
424.10 |
|
Mean |
- |
423.79 |
|
CV% |
- |
2 |
*Corrected for blank absorbance value
R7-R9= Replicates 7 to 9
R13-R18= Replicates 13 to 18
CV = Coefficient of Variation
Table 3: Inhibition of Nitrogen Transformation Activity
Nominal Concentration |
Day 7 |
Day 28 |
||
Nitrate Formation Rate |
% Inhibition |
Nitrate Formation Rate |
% Inhibition |
|
Control |
11.61 |
- |
11.24 |
- |
1000 |
11.01 |
5 |
11.21 |
0 |
Description of key information
- EC50 (28d): >1000 mg/kg dw test mat. (nominal) based on: nitrate formation rate
- NOEC (28d): ≥1000 mg/kg dw test mat. (nominal) based on: nitrate formation rate
Key value for chemical safety assessment
Additional information
A nitrogen transformation test is available for the read across substance GTL Base Oil Distillates. The test was conducted in accordance with OECD 216 and GLP.
Soil microorganisms were exposed to the substance at concentrations of 1000 mg/kg (dry weight) for 28 days at a temperature of 21°C, in the dark, on natural soil with an organic carbon content of 0.97 ± 0.07% with addition of Lucerne-green-grass meal as a respiratory substrate. The inhibitory effect of the test substance on nitrogen transformation was determined by measurement of nitrate concentrations in soil samples taken on Days 7, 14, 21 and 28, in comparison to control samples.
The effect of the test substance on nitrogen transformation gave an EC50greater than 1000 mg/kg dw.
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