4-trans-Pentyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 30, 2010 - December 09, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Macro nutrients (mg/L)CaCI2 •2H20293.80MgSO4•7H20123.30NaHCO364.80KCI5.80Na2SiO3•9H2010.00NaNO30.27KH2PO40.14K2HPO40.18Trace elementsmg/LB0.5000Fe0.2000Mn0.1000Li, Rb and Sr0.0500Mo0.0250Br0.0125Cu and Zn0.0063Co and I0.0025Se0.0010V0.0003Macro nutrientsNa2EDTA • 2H202.50Vitamins (µg/L):Thiamine75.00B121.00Biotin0.75- pH: 8 ± 0.3- hardness 14 ° dH (250 mg/L CaCO3)

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Daphnia- Strain/clone: Daphnia Magna- Source: in house- Age of parental stock: younger than 24 hours - Feeding during test: noAt the start of the experimental phase 5 Daphnia magna were placed in each test vessel containing 20 ml reconstituted water with or without test material. The test vessels were left open.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

14o dH (about 250 mg/L CaCO3)

Test temperature:

18 – 22°C

pH:

7.9 +/- 0.3

Dissolved oxygen:

at least 90 %

Nominal and measured concentrations:

Nominal 100 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: - Type: open static - Material: test item in reconstituted water - Aeration: 24 hours after preparation - No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4- Biomass loading rate: --TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: reconstituted according to ELENDT (1990)Macro nutrientsmg/LCaCl2 x 2H2O 293.80MgSO4 x 7H2 123.30NaHCO3 64.80KCl5.80Na2SiO3 9H2O10.00NaNO30.27KH2PO40.14K2HPO40.18Trace elementsmg/LB0.5000Fe0.2000Mn0.1000Li, Rb and Sr0.0500Mo0.0250Br0.0125Cu and Zn0.0063Co and I0.0025Se0.0010V0.0003Macro nutrientsNa2EDTA  2H2O2.50Vitamins µg/LThiamine75.00B121.00Biotin0.75- Total organic carbon: --- Particulate matter: --- Metals: --- Pesticides: --- Chlorine: --- Alkalinity: --- Ca/mg ratio: --- Conductivity: --- Culture medium different from test medium: no- Intervals of water quality measurement: 48 hOTHER TEST CONDITIONS- Adjustment of pH: 7.9- Photoperiod: 16h light / 8h dark- Light intensity: --EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : TEST CONCENTRATIONS- Spacing factor for test concentrations: - Justification for using less concentrations than requested by guideline: - Range finding study - Test concentrations: - Results used to determine the conditions for the definitive study: References:ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.Protoplasma 154, 25-33, 1990

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In this open static test system, Daphnia magna exposed to a nominal concentration of 100 mg/L revealed no biological effects.

Results with reference substance (positive control):

Potassium dichromate: 24 h EC50 0.95 (0.87-1.04) mg/L48 h EC50 0.74 (0.68-0.81) mg/L

Validity criteria fulfilled:

yes

Conclusions:

Under the given experimental conditions, the test material showed no aquatic toxicity up to the limit of water solubility. The 48 hours EC50 to Daphnia magna is > 100 mg/L.

Executive summary:

Purpose

The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.

Study design

For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The GLP study was performed as a limit test according to OECD TG 202.

Results

The follwing results have been obtained:

   

Nominal concentration Immobilization immobilized / exposed
[g/L]	24 hours	48 hours
0.0	0/20	0/20
0.1	0/20	0/20

Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg per L of test item were not affected.

For the test material the following EC50 values for Daphnia magna were determined:

24 h EC50 > 0,1 g/L (nominal > 100 mg/L)

48 h EC50 > 0,1 g/L (nominal > 100 mg/L)

NOEC > 0.1 g/L (nominal)

LOEC > 0.1 g/L (nominal)

Conclusions

Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC to Daphnia magna is > 100 mg/L.

Description of key information

Under the given experimental conditions, the test material showed no aquatic toxicity up to the limit of water solubility. The 48 hours EC50 to Daphnia magna is > 100 mg/L.