4-methylpentan-2-one oxime

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 May 2003 until 14 August 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-methylpentan-2-one oxime

EC Number:

203-298-2

EC Name:

4-methylpentan-2-one oxime

Cas Number:

105-44-2

Molecular formula:

C6H13NO

IUPAC Name:

4-methylpentan-2-one oxime

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: S285
- Expiration date of the lot/batch: 16 May 2004
- purity: > 99%
- appearance: clear colourless liquid

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ca 4°C in darkness

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 4.6, 10, 22, 46 and 100 mg/l
- Sampling method: Duplicate 50 ml samples were taken from the control and each test concentration at 0 hours (main stock solutions) and after 48 hours (replicates pooled) for analysis.
- Sample storage conditions before analysis: not indicated

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance, 200 mg, was dissolved in 2.0 litres of Elendt M4 to produce a solution of 100 mg/l, which was used for the highest test concentration. Serial dilutions of this solution were prepared with test medium to give the remaining nominal test concentrations of 4.6, 10, 22 and 46 mg/l.
- Controls: yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: strain originating from the Institute National de recherche Chimique Appliqué (IRChA), France
- Source: in-house culture
- Age of parental stock (mean and range, SD): The day before the start of the study, all juvenile Daphnia were removed from the laboratory cultures. The following morning, juveniles produced by the gravid (egg-bearing) adult Daphnia were removed from the culture vessels and held in a separate holding vessel; these animals, which were less than 24 hours old, were used in the test.
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

not indicated

Test temperature:

21°C

pH:

7.4 - 7.6

Dissolved oxygen:

7.4 - 8.1 mg/l

Salinity:

not relevant

Conductivity:

not relevant

Nominal and measured concentrations:

Nominal concentration: control, 4.6, 10, 22, 46, 100 mg/l
Measured concentration (T=0 hours): ND*, 4.38, 9.68, 20.8, 43.6, 95.7 mg/l
Measured concentration (T= 48 hours): ND*, 4.12, 9.16, 19.8, 41.3, 89.9 mg/l

*ND: none detected (limit of detection: 0.04 mg/l)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass jars
- Type (delete if not applicable): the jars were loosely covered to minimise evaporative losses
- Material, size, headspace, fill volume: 250 ml capacity glass jars containing 100 ml of prepared medium
- Aeration: not during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- The test organisms were maintained and the tests conducted in a reconstituted medium Elendt M4. The medium as prepared using analytical grade reagents and reverse osmosis deionised water.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: not indicated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations of the condition of the test and control Daphnia were made following 24 and 48 hours exposure. Daphnia were considered to be immobilised if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel.

RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1, 1.0, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: yes (EC 50> 100 mg/L)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 93 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none indicated
- Mortality of control: no
- Abnormal responses: none indicated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none indicated

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of MIBKO to Daphnia magna was determined in a 48-hour static test according to OECD guideline 202. The 48-hour EC50 was calculated to be >93 mg/L (based on the geometric mean measured concentrations). The results of the test can be considered reliable without restriction.