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EC number: 444-340-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 - 27 Apr 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- MV31-K-salt
- IUPAC Name:
- MV31-K-salt
- Details on test material:
- - Name of test material (as cited in study report): MV31 K-salt, Potassiumperfluoromethoxypropoxypropionate
- Substance type: Pure active substance
- Physical state: solid, grey granules
- Analytical purity: ca. 88 %
- Storage condition of test material: darkness at approximately 20 °C in a fume cupboard
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 20 mL samples were taken form all test solutions at study initiation, and after 24 and 72 hours. Test results appear to be from composite samples.
- Sample storage conditions before analysis: No information
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution prepared by dissolving the substance in deionized water. The chosen test concentration was prepared by adding suitable aliquots to algae pre-cultures.
- Controls: Water blank
- Evidence of undissolved material: None. Test solutions were clear.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: SAG Strain No. 86.81
- Source: Culture maintained at test laboratory. Origin: Albrecht-von-Haller-Institute for Plant Sciences, Department of Experimental Phycology and Collection of Algal Cultures, University of Gottingen, Germany
- Age of inoculum (at test initiation):
- Method of cultivation: As per Appendix 1 of test guideline
ACCLIMATION
- Acclimation period: No
- Culturing media and conditions: Same as test
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23.0 - 23.6 °C
- pH:
- Initial: 7.8 - 7.8. Final: 10.7
- Nominal and measured concentrations:
- Nominal: 0 mg/L (control), 100 mg/L
Measured: < LOD, 104.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Aeration: Stirring or bubbling
- Initial cells density: ca. 10,000 cells/mL
- Control end cells density: 1039000 cells/ml
- No. of vessels per concentration (replicates): three
- No. of vessels per control (replicates): six
GROWTH MEDIUM
- Standard medium used: yes, OECD medium with iron chloride (FeCl3·6(H2O)) increased from 64 mg/L to 80 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled water or deionized water with a conductivity of <5 µS/cm was used to make the growth medium.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 6,000 to 10,000 lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: cell density in each flask was determined at 24, 48 and 72 hours after the start of the test, using a cell counter.
- Other: algae were observed microscopically at the beginning and at the end of the study. No visible changes of the shape of the algae were noticed either in the control or the treatment groups.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: none, limit test
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 4% inhibition
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- (area under growth curve)
- Remarks on result:
- other: 14% inhibition
- Details on results:
- - Exponential growth in the control: yes (Table 1)
- Observation of abnormalities: no abnormalities observed, all cells normal
- Any stimulation of growth found in any treatment: no
- Effect concentrations exceeding solubility of substance in test medium: no (Table 2) - Results with reference substance (positive control):
- Testing lab does twice-annual testing on potassium dichromate, date and results of most recent test not stated.
- Reported statistics and error estimates:
- Effects on biomass (as area under growth curve) and growth rate were statistically significant by one-sided t-test (α = 0.05). The NOEC cannot be derived in this test.
Any other information on results incl. tables
Table 1: Individual data from algae inhibition growth test |
|||||||
Concentration (mg) |
Cell counts (cells/ mL) |
Section specific growth rates (1/day) |
Average growth rate (1/day) |
||||
24 h |
48 h |
72 h |
0-24 h |
24-48 h |
48-72h |
||
0 |
45640 |
219400 |
1093667 |
1.51820 |
1.59268 |
1.65350 |
1.56490 |
0 |
46470 |
211500 |
1051667 |
1.53622 |
1.55601 |
1.61434 |
1.55185 |
0 |
46407 |
219800 |
1083000 |
1.53487 |
1.59450 |
1.64369 |
1.56164 |
0 |
42093 |
195200 |
996133 |
1.43730 |
1.47581 |
1.56009 |
1.53377 |
0 |
44453 |
203967 |
1004833 |
1.49185 |
1.51974 |
1.56878 |
1.53666 |
0 |
42667 |
205967 |
1002033 |
1.45084 |
1.52950 |
1.56599 |
1.53573 |
Mean |
44622 |
209306 |
1038556 |
1.49488 |
1.54470 |
1.60106 |
1.54742 |
100 |
45737 |
203500 |
881700 |
1.52032 |
1.53704 |
1.51113 |
1.49309 |
100 |
43950 |
192200 |
862900 |
1.48047 |
1.47992 |
1.48958 |
1.48590 |
100 |
41580 |
187967 |
828167 |
1.42503 |
1.45765 |
1.44850 |
1.47221 |
Mean |
43756 |
194556 |
857589 |
1.47527 |
1.49154 |
1.48307 |
1.48373 |
Mean coefficient of variation for section-by-section specific growth rates: 3.5% |
|||||||
Coefficient of variation of average specific growth rates, 0.9% |
Table 2, Concentrations during algae tox test |
|||||
Sample No. |
Nominal concentration |
Sampling day |
Mean measured concentration (mg/L) ¹ |
||
1 |
0 mg/L (control) |
0 |
<LOD ² |
─ |
─ |
2 |
0 mg/L |
1 |
─ |
<LOD |
─ |
3 |
0 mg/L |
3 |
─ |
─ |
<LOD |
4 |
100 mg/L |
0 |
104 |
─ |
─ |
5 |
100 mg/L |
1 |
─ |
103 |
─ |
6 |
100 mg/L |
3 |
─ |
─ |
106 |
1; mean of two determinations |
|||||
2; LOD, 1 mg/L |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Control cell density increased by a factor >16 within 3 days. Concentrations of the test substance maintained within 80 % of the initial throughout test.
- Conclusions:
- The 72-hour EC50 (growth rate) of MV31-K-salt is >100 mg/L (EC method C.3)
- Executive summary:
Toxicity of MV31-K-salt was assessed in a limit test done according to EC method C.3. 100 mg/L solutions of MV31-K-salt were tested in triplicate v. 6 controls. A statistically significant (α = 0.5) effect was observed on biomass as area under growth curve among test substance replicates. A 4% effect was observed on growth rate which was statistically significant but not biologically relevant. The 72-hour EC50 of MV31-K-salt is >100 mg/L. The NOEC could not be determined.
The study was conducted under internationally accepted guidelines and was GLP compliant. It is reliable without restrictions and is suitable for Risk Assessment, Classification & Labelling, and PBT Analysis.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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