Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-002-7 | CAS number: 24057-28-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples were taken from each treatment at the start and at the end of the two test medium renewal periods.
All samples were stored frozen (at about -20 ± 5 C) immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An aqueous solution with a nominal content of 80 % was prepared prior to the test. The test substance content of this working solution was analytically verified by HPLC to be 77.9 % (water-free). The test medium with a nominal concentration of 8.6 mg/L was prepared by completely diluting 55 mg of the working solution (corresponding to 42.8 mg water-free test substance) in 5.0 L of test water by intensive stirring at room temperature for 30 minutes in the dark. The test medium was prepared just before the introduction of the fish (i.e., start of the test and at the test medium renewal).
- Controls: test water - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Breeding culture at the testing laboratory
- Length at study initiation(length definition, mean, range and SD): measured from the mouth to the end of the tail fin, 2.7 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.12 ± 0.02 g
- Feeding during test: none
HOLDING AND ACCLIMATION
No medication was applied during holding and acclimatization.
- Acclimation period: ≥ 1 week
- Acclimation conditions (same as test or not): same
- Feeding during acclimation: until one day before the start of the test, the fish were fed with a commercial fish diet (Tetra Min® Hauptfutter, TETRA-Werke, 49324 Melle / Germany)
- Health during acclimation (any mortality observed): During the last four weeks prior to the test, no fish died in the test fish batch and all fish were healthy.
FEEDING DURING TEST
none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 1.25 mM (125 mg CaCO3/L)
- Test temperature:
- 22 °C
- pH:
- 7.2 - 7.4
- Dissolved oxygen:
- ≥ 8.2 mg/L (≥ 93 % oxygen saturation)
- Nominal and measured concentrations:
- nominal concentration: 8.6 mg/L (water-free)
measured concentrations:
freshly prepared test media: 114-116 % of nominal
aged test media (48 h): 119-123 % of nominal
The biological results are based on the mean measured concentration of 10.1 mg/L (calculated as arithmetic mean over all concentrations measured at the start and the end of the two test medium renewal periods). - Details on test conditions:
- TEST SYSTEM
- Test vessel: full glass aquarium, containing five liters test medium
- Type (delete if not applicable): open
- Aeration: slight aeration
- Renewal rate of test solution (frequency/flow rate): after 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.17 g fish wet weight/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water
- Alkalinity: 0.4 mM
- Ca/Mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: water temperature, pH and oxygen concentration were measured at the start of the test and once during the test in the freshly prepared and aged test medium. At the same dates the appearance of the test medium was recorded.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hour light to 8-hour dark photoperiod, with a 30-minute transition period
- Light intensity 15 – 16 µE s-1 m-2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and sub-lethal effects; recorded at approx. 3, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: A limit test based on the threshold approach was performed (OECD Series on testing and assessment No. 126 (2010): Short Guidance on the Threshold Approach for Acute Fish Toxicity). The selected concentration of nominal 8.6 mg/L water-free test item (mean measured 10.1 mg/L) was based on the EC50 values determined in an algae study (OECD 201, EC C.3) and an acute daphnia study (OECD 202, EC C.2). The lower EC50 value was determined in the algae test to be 3.0 mg/L, based on growth rate (EC50 for daphnia was >100 mg/L). - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Remarks:
- corrected for water content
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- highest concentration tested in a threshold approach
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Remarks:
- corrected for water content
- Basis for effect:
- other: mortality and sub-lethal effects
- Remarks on result:
- other:
- Remarks:
- highest concentration tested in a threshold approach
- Details on results:
- - Behavioural abnormalities:
- Other biological observations: none; all fish survived and no visible abnormalities were observed.
- Mortality of control: none
- Other adverse effects control: none
The test medium was a clear solution throughout the test medium renewal periods of 48 hours. - Reported statistics and error estimates:
- The NOEC and LC0 were determined directly from the raw data. The LOEC, LC100 and LC50 at the observation times could not be quantified due to the absence of a toxic effect of the test item at the tested threshold concentration.
- Validity criteria fulfilled:
- yes
- Remarks:
- / mortality of control animals <10 %; concentration of dissolved oxygen in all test vessels >60 % saturation
Reference
Description of key information
96-h LC50 > 10 mg/L for Zebrafish (Danio rerio) (OECD 203, EU C.1; threshold approach)
Key value for chemical safety assessment
Additional information
LC50 for freshwater fish: > 10 mg/L (threshold approach)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.