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EC number: 218-746-2 | CAS number: 2224-15-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2'-[ethylenebis(oxymethylene)]bisoxirane
- EC Number:
- 218-746-2
- EC Name:
- 2,2'-[ethylenebis(oxymethylene)]bisoxirane
- Cas Number:
- 2224-15-9
- Molecular formula:
- C8H14O4
- IUPAC Name:
- 2,2'-[ethylenebis(oxymethylene)]bisoxirane
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: secondary effluent of public sewage treatment facility
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The secondary effluent of Jinju Munsan public sewage treatment facility
- Storage conditions: After transportation of the inoculum, it was aerated for 6 days at 22.0±2.0˚C before use. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2.0°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: test bottle
- Number of culture flasks/concentration: 2
- Measuring equipment: BOD meter: WTW, Oxitop® IS12, Germany ; WTW, Oxitop® OC100, Germany
SAMPLING
- Sampling frequency: The oxygen uptake for all bottles was measured automatically by BOD meter and recorded daily over a period of 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: mineral medium + inoculum
- Procedure control: reference item + mineral medium + inoculum
- Abiotic sterile control: test item + mineral medium + sterilising agent (HgCl2)
- Toxicity control: test item + reference item + mineral medium + inoculum
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.6
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The coefficients of variation of the test suspensions (Bottle 1, 2) were more than 20% at the end of the test due to low BOD concentration. Though this value was higher than guideline specifications, it did not affect the interpretation of results.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The percentage microbial degradation of the reference item, sodium benzoate was 86.2% at 14 days. These value was within the acceptable range (60% of ThOD within 14 days) specified by the OECD Guidelines and the National Institute of Environmental Research Guidelines.
The oxygen uptake of the inoculum blank (mean of Bottle 3, 4) for 28 days ranged from 0.0 to 12.7 mg O2/L. The pH value of the test suspensions (Bottle 1, 2) were 7.6 at the end of the test. All values met the criteria of test guidelines. Toxicity control (Bottle 7) showed test item had obviously no inhibitory effect on the activity of the inoculum.
The percentage microbial degradation of the test item based on the theoretical oxygen demand (ThOD) was 1.6±1.1% at 28 days. Therefore 2,2’-[ethylenebis(oxymethylene)]bisoxiranecould not be regarded as readily biodegradable in the aquatic environment based on the pass level of biodegradability (60%). - Executive summary:
The purpose of the study was to evaluate the biodegradability of the test item, 2,2’-[ethylenebis(oxymethylene)]bisoxirane, in the aquatic environment. Microbial degradation test was performed for 28 days in compliance with the OECD Guidelines and the National Institute of Environmental Research Guidelines.
The results are as follows:
1) The test temperature during the test period was maintained at 22.3±0.3˚C and the pH values of test suspensions were 7.6 at 28 days. These values met specifications of the test guidelines (22.0±2.0˚C, pH 6.0~8.5).
2) The percentage microbial degradation of the reference item (sodium benzoate) based on the theoretical oxygen demand (ThOD) was 86.2% at 14 days, indicating that the activity of the inoculum was suitable for this test.
3) The percentage microbial degradation of the toxicity control based on ThOD was 46.0% at 14 days, indicating that the test item had obviously no inhibitory effect on the activity of the inoculum.
4) The percentage microbial degradation of the test item based on the ThOD was 1.6±1.1% at 28 days.
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